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pgl3 basic  (Addgene inc)


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    Structured Review

    Addgene inc pgl3 basic
    Pgl3 Basic, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3-basic+vector/pGL3+Basic+Control+Vector+(Plasmid+%23137707)/pm41713257-68-22-27
    Average 93 stars, based on 49 article reviews
    pgl3 basic - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Binding Assay:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Plasmid Preparation:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control. .. The protein concentrations were ascertained through the Bradford protein assay (Bio-Rad Laboratories, Milano, Italy).

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.

    Construct:

    Article Title: Bmal1 Regulates Vascular Calcification via Non-Canonical Circadian Pathway
    Article Snippet: The aortic expression of Runx2 and SMA in arteries from the STZ-treated Bmal1 flox/flox and Bmal1 Δ/Δ mice were determined by immunofluorescent staining of consecutive 7-μm aortic sections using specific primary antibodies (Runx2, MBL Cat# D130–3 and SMA, Sigma Cat# A5228). .. To assess a direct regulation of Bmal1 on Runx2 transcription, a 1,974-bp mouse Runx2 promoter (mm10: chr17:44,735,972–44,737,945) containing putative Bmal1 binding sites was insert into the pGL3-Basic vector (Addgene) to generate the wildtype mRunx2 promoter reporter construct (Bmal1 BS Mut −). .. Using this construct, the CACGTG Bmal1 binding site (chr17:44,737,386–44,737,391) within the mRunx2 promoter was deleted using the QuikChange Lightning Multi-Site-Directed Mutagenesis Kit (Agilent Technologies) to generate mutant mRunx2 promoter reporter construct (Bmal1 BS Mut +).

    Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma.
    Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis.

    Transfection:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Control:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: β-catenin: A crucial transcriptional activator of KSHV latency genes and small molecule target in primary effusion lymphoma.
    Article Snippet: Latency-associated nuclear antigen (LANA)-induced β-catenin accumulation is one of the prime events in the Kaposi sarcoma-associated herpesvirus (KSHV)-associated primary effusion lymphoma (PEL).. LANA interacts with GSK3β, which in turn increases β-catenin pool in this lymphoma.. The intrinsic molecular mechanism and the effects of β-catenin accumulation in this lymphoma is still unclear. β-catenin acts as a major transcription factor that regulates a variety of proliferative signaling pathways and promotes oncogenesis.

    Article Title: Long-lasting Avoidance Induced by Repeated Stimulation of the Rostromedial Tegmental Nucleus
    Article Snippet: .. Retrogradely-transported virus encoding cre recombinase (pENN.AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40; Addgene, Watertown, MA) was injected into the VTA (150nL/hemisphere), and cre-dependent excitatory designer receptors exclusively activated by designer drugs (DREADDs; AAV-hSyn-DIO-hM3D(Gq)-mCherry; Addgene, Watertown, MA) or control vector (pAAV2-hSyn-DIO-mCherry; Addgene, Watertown, MA) was injected bilaterally into the RMTg (400nL/hemisphere). ..

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. pGL3-human Oct4 PE-SV40-luc was purchase from Addgene; the vector pGL3 bought from Pomega was used as a control. .. The protein concentrations were ascertained through the Bradford protein assay (Bio-Rad Laboratories, Milano, Italy).

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.

    Luciferase:

    Article Title: The NSP6-L260F substitution in SARS-CoV-2 BQ.1.1 and XBB.1.16 lineages compensates for the reduced viral polymerase activity caused by mutations in NSP13 and NSP14
    Article Snippet: .. The BACs encoding the replicative cDNA and pGL3-control vector were transfected into HEK293T cells using TransIT-LT1 (TaKaRa) according to the manufacturer’s instructions. pGL3 Control Vector, a gift from Debrya Groskreutz (Addgene plasmid #212937; http://n2t.net/addgene:212937 ; RRID: Addgene_212937), expressed firefly luciferase and served as an internal control. .. After incubation for 72 h at 37°C, the transfected cells were lysed with Passive Lysis buffer (Promega).

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Virus:

    Article Title: Long-lasting Avoidance Induced by Repeated Stimulation of the Rostromedial Tegmental Nucleus
    Article Snippet: .. Retrogradely-transported virus encoding cre recombinase (pENN.AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40; Addgene, Watertown, MA) was injected into the VTA (150nL/hemisphere), and cre-dependent excitatory designer receptors exclusively activated by designer drugs (DREADDs; AAV-hSyn-DIO-hM3D(Gq)-mCherry; Addgene, Watertown, MA) or control vector (pAAV2-hSyn-DIO-mCherry; Addgene, Watertown, MA) was injected bilaterally into the RMTg (400nL/hemisphere). ..

    Injection:

    Article Title: Long-lasting Avoidance Induced by Repeated Stimulation of the Rostromedial Tegmental Nucleus
    Article Snippet: .. Retrogradely-transported virus encoding cre recombinase (pENN.AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40; Addgene, Watertown, MA) was injected into the VTA (150nL/hemisphere), and cre-dependent excitatory designer receptors exclusively activated by designer drugs (DREADDs; AAV-hSyn-DIO-hM3D(Gq)-mCherry; Addgene, Watertown, MA) or control vector (pAAV2-hSyn-DIO-mCherry; Addgene, Watertown, MA) was injected bilaterally into the RMTg (400nL/hemisphere). ..

    Bioprocessing:

    Article Title: Long-lasting Avoidance Induced by Repeated Stimulation of the Rostromedial Tegmental Nucleus
    Article Snippet: .. Retrogradely-transported virus encoding cre recombinase (pENN.AAVrg.hSyn.HI.eGFP-Cre.WPRE.SV40; Addgene, Watertown, MA) was injected into the VTA (150nL/hemisphere), and cre-dependent excitatory designer receptors exclusively activated by designer drugs (DREADDs; AAV-hSyn-DIO-hM3D(Gq)-mCherry; Addgene, Watertown, MA) or control vector (pAAV2-hSyn-DIO-mCherry; Addgene, Watertown, MA) was injected bilaterally into the RMTg (400nL/hemisphere). ..

    other:

    Article Title: E2F1 suppresses Epstein-Barr virus lytic reactivation through cellular and viral transcriptional networks
    Article Snippet: pGL3 Basic Vector , Gift from Debrya Groskreutz , RRID: Addgene_212936.

    Activity Assay:

    Article Title: WNT3 promotes chemoresistance to oxaliplatin in oral squamous cell carcinoma via regulating ABCG2 expression
    Article Snippet: .. To assess Wnt/β-catenin signaling activity, cells were co-transfected with either WNT3 or control Vector plasmids, along with the TopFlash/FOPFlash plasmid and the Renilla luciferase internal control plasmid (Addgene, USA). ..

    Generated:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.

    shRNA:

    Article Title: Supporting Information for Extrachromosomal Telomere DNA Derived from Excessive Strand Displacements
    Article Snippet: .. Lentiviruses were generated by transfecting shRNA vectors targeting human POLD3 (6), STN1 (7), ATRX(8), or the control vector, or sgRNA vectors targeting human PML exon 3 (sense: CACCGGcggtaccagcgcgactacg, antisense: AAACcgtagtcgcgctggtaccgCC) inserted in pLentiV2 vector (Addgene, 52961) along with pMD2.G and psPAX into 293T cells. .. Transfection was carried out using TransIT (Mirus) following the manufacturer's protocol.



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