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pgl3-basic luciferase reporter vector (ptfam/luc)  (Pomega Inc)

 
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    Structured Review

    Pomega Inc pgl3-basic luciferase reporter vector (ptfam/luc)
    Pgl3 Basic Luciferase Reporter Vector (Ptfam/Luc), supplied by Pomega Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl3+basic+vector/pgl3+basic+luciferase+reporter+vector++ptfam+luc+/pmc04012862-237-38-43
    Average 90 stars, based on 1 article reviews
    pgl3-basic luciferase reporter vector (ptfam/luc) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Staining:

    Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
    Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with water gently, pictures were taken after air dried at room temperature. . (e) Luciferase reporter assay Oligonucleotide fragments containing six tandem FKHD-consensus (canonical or non-canonical) ( Adams et al., 2019 ) motifs with 5-bp spacers (Oligo list) were cloned into pGL3-Promoter Luciferase Reporter Vector (Pomega) between NheI and XhoI restriction sites.

    Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression
    Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the pGL3-Basic luciferase reporter vector (pTFAM/LUC) (Pomega Corp).

    Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
    Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

    Luciferase:

    Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
    Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with water gently, pictures were taken after air dried at room temperature. . (e) Luciferase reporter assay Oligonucleotide fragments containing six tandem FKHD-consensus (canonical or non-canonical) ( Adams et al., 2019 ) motifs with 5-bp spacers (Oligo list) were cloned into pGL3-Promoter Luciferase Reporter Vector (Pomega) between NheI and XhoI restriction sites.

    Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression
    Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the pGL3-Basic luciferase reporter vector (pTFAM/LUC) (Pomega Corp).

    Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
    Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

    Reporter Assay:

    Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
    Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with water gently, pictures were taken after air dried at room temperature. . (e) Luciferase reporter assay Oligonucleotide fragments containing six tandem FKHD-consensus (canonical or non-canonical) ( Adams et al., 2019 ) motifs with 5-bp spacers (Oligo list) were cloned into pGL3-Promoter Luciferase Reporter Vector (Pomega) between NheI and XhoI restriction sites.

    Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression
    Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the pGL3-Basic luciferase reporter vector (pTFAM/LUC) (Pomega Corp).

    Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
    Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

    Clone Assay:

    Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
    Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with water gently, pictures were taken after air dried at room temperature. . (e) Luciferase reporter assay Oligonucleotide fragments containing six tandem FKHD-consensus (canonical or non-canonical) ( Adams et al., 2019 ) motifs with 5-bp spacers (Oligo list) were cloned into pGL3-Promoter Luciferase Reporter Vector (Pomega) between NheI and XhoI restriction sites.

    Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression
    Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the pGL3-Basic luciferase reporter vector (pTFAM/LUC) (Pomega Corp).

    Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
    Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

    Plasmid Preparation:

    Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
    Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with water gently, pictures were taken after air dried at room temperature. . (e) Luciferase reporter assay Oligonucleotide fragments containing six tandem FKHD-consensus (canonical or non-canonical) ( Adams et al., 2019 ) motifs with 5-bp spacers (Oligo list) were cloned into pGL3-Promoter Luciferase Reporter Vector (Pomega) between NheI and XhoI restriction sites.

    Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression
    Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the pGL3-Basic luciferase reporter vector (pTFAM/LUC) (Pomega Corp).

    Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
    Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA

    Purification:

    Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes
    Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with water gently, pictures were taken after air dried at room temperature. . (e) Luciferase reporter assay Oligonucleotide fragments containing six tandem FKHD-consensus (canonical or non-canonical) ( Adams et al., 2019 ) motifs with 5-bp spacers (Oligo list) were cloned into pGL3-Promoter Luciferase Reporter Vector (Pomega) between NheI and XhoI restriction sites.

    Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression
    Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the pGL3-Basic luciferase reporter vector (pTFAM/LUC) (Pomega Corp).

    Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species.
    Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA



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