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Structured Review

SunBio Inc pgl3-mmp-2 promoter construct
Pgl3 Mmp 2 Promoter Construct, supplied by SunBio Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+basic+vector/pgl3+basic+luciferase+reporter+vector/pmc03875455-86-3-8
Average 90 stars, based on 1 article reviews
pgl3-mmp-2 promoter construct - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

other:

Article Title: POU5F1 Enhances the Invasiveness of Cancer Stem-Like Cells in Lung Adenocarcinoma by Upregulation of MMP-2 Expression
Article Snippet: The sequence of pGL3-MMP-2 promoter construct was confirmed (Sunbio Medical Biotechnology, China).

Luciferase:

Article Title: Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D
Article Snippet: .. For luciferase reporter assays, the NUSAP1 promoter containing the MEF2D-binding site (from 1,519 to 1,508 bp) was amplified from A549 cells by PCR and cloned into the pGL3-Basic luciferase reporter vector by SunBio, Inc. A mutated NUSAP1 promoter (pNUSAP1-mut) with deletion of the MEF2D-binding site was also generated by SunBio, Inc. H1299 and A549 cells were co-transfected with a universal shNC or MEF2D shRNA (MEF2D RNAi) and wild-type NUSAP1 luciferase reporter vector (pNUSAP1-luc). .. In addition, HCC827 cells were co-transfected with a MEF2D pcDNA3.1 vector (MEF2D) or a pcDNA3.1 control vector (pcDNA3.1) and pNUSAP1-luc or pNUSAP1-mut.

Amplification:

Article Title: Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D
Article Snippet: .. For luciferase reporter assays, the NUSAP1 promoter containing the MEF2D-binding site (from 1,519 to 1,508 bp) was amplified from A549 cells by PCR and cloned into the pGL3-Basic luciferase reporter vector by SunBio, Inc. A mutated NUSAP1 promoter (pNUSAP1-mut) with deletion of the MEF2D-binding site was also generated by SunBio, Inc. H1299 and A549 cells were co-transfected with a universal shNC or MEF2D shRNA (MEF2D RNAi) and wild-type NUSAP1 luciferase reporter vector (pNUSAP1-luc). .. In addition, HCC827 cells were co-transfected with a MEF2D pcDNA3.1 vector (MEF2D) or a pcDNA3.1 control vector (pcDNA3.1) and pNUSAP1-luc or pNUSAP1-mut.

Polymerase Chain Reaction:

Article Title: Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D
Article Snippet: .. For luciferase reporter assays, the NUSAP1 promoter containing the MEF2D-binding site (from 1,519 to 1,508 bp) was amplified from A549 cells by PCR and cloned into the pGL3-Basic luciferase reporter vector by SunBio, Inc. A mutated NUSAP1 promoter (pNUSAP1-mut) with deletion of the MEF2D-binding site was also generated by SunBio, Inc. H1299 and A549 cells were co-transfected with a universal shNC or MEF2D shRNA (MEF2D RNAi) and wild-type NUSAP1 luciferase reporter vector (pNUSAP1-luc). .. In addition, HCC827 cells were co-transfected with a MEF2D pcDNA3.1 vector (MEF2D) or a pcDNA3.1 control vector (pcDNA3.1) and pNUSAP1-luc or pNUSAP1-mut.

Clone Assay:

Article Title: Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D
Article Snippet: .. For luciferase reporter assays, the NUSAP1 promoter containing the MEF2D-binding site (from 1,519 to 1,508 bp) was amplified from A549 cells by PCR and cloned into the pGL3-Basic luciferase reporter vector by SunBio, Inc. A mutated NUSAP1 promoter (pNUSAP1-mut) with deletion of the MEF2D-binding site was also generated by SunBio, Inc. H1299 and A549 cells were co-transfected with a universal shNC or MEF2D shRNA (MEF2D RNAi) and wild-type NUSAP1 luciferase reporter vector (pNUSAP1-luc). .. In addition, HCC827 cells were co-transfected with a MEF2D pcDNA3.1 vector (MEF2D) or a pcDNA3.1 control vector (pcDNA3.1) and pNUSAP1-luc or pNUSAP1-mut.

Generated:

Article Title: Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D
Article Snippet: .. For luciferase reporter assays, the NUSAP1 promoter containing the MEF2D-binding site (from 1,519 to 1,508 bp) was amplified from A549 cells by PCR and cloned into the pGL3-Basic luciferase reporter vector by SunBio, Inc. A mutated NUSAP1 promoter (pNUSAP1-mut) with deletion of the MEF2D-binding site was also generated by SunBio, Inc. H1299 and A549 cells were co-transfected with a universal shNC or MEF2D shRNA (MEF2D RNAi) and wild-type NUSAP1 luciferase reporter vector (pNUSAP1-luc). .. In addition, HCC827 cells were co-transfected with a MEF2D pcDNA3.1 vector (MEF2D) or a pcDNA3.1 control vector (pcDNA3.1) and pNUSAP1-luc or pNUSAP1-mut.

shRNA:

Article Title: Nucleolar and spindle-associated protein 1 promotes non-small cell lung cancer progression and serves as an effector of myocyte enhancer factor 2D
Article Snippet: .. For luciferase reporter assays, the NUSAP1 promoter containing the MEF2D-binding site (from 1,519 to 1,508 bp) was amplified from A549 cells by PCR and cloned into the pGL3-Basic luciferase reporter vector by SunBio, Inc. A mutated NUSAP1 promoter (pNUSAP1-mut) with deletion of the MEF2D-binding site was also generated by SunBio, Inc. H1299 and A549 cells were co-transfected with a universal shNC or MEF2D shRNA (MEF2D RNAi) and wild-type NUSAP1 luciferase reporter vector (pNUSAP1-luc). .. In addition, HCC827 cells were co-transfected with a MEF2D pcDNA3.1 vector (MEF2D) or a pcDNA3.1 control vector (pcDNA3.1) and pNUSAP1-luc or pNUSAP1-mut.



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