pgl3-basic luciferase reporter vector (ptfam/luc) (Pomega Inc)
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Pgl3 Basic Luciferase Reporter Vector (Ptfam/Luc), supplied by Pomega Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3+basic+empty+vector/pgl3+basic+luciferase+reporter+vector++ptfam+luc+/pmc04012862-237-38-43
Average 90 stars, based on 1 article reviews
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Staining:Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species. Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA Luciferase:Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species. Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA Reporter Assay:Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species. Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA Clone Assay:Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species. Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA Plasmid Preparation:Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species. Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA Purification:Article Title: A pan-cancer transcriptome analysis of exitron splicing identifies novel cancer driver genes and neoepitopes Article Snippet: Following PBS wash, cells were stained using 0.1% crystal violet solution at room temperature for 30 min. Stained cells were washed with Article Title: Pdx1 deficiency causes mitochondrial dysfunction and defective insulin secretion through TFAM suppression Article Snippet: The tfam gene promoter fragment was then excised using the restriction enzyme Eco R1, made blunt ended using the Klenow fragment of the DNA polymerase I, gel purified and subcloned into the Sma I restriction site of the Article Title: Total phenolic contents, free radical scavenging activities and inhibitory effects on the activation of NF-kappa B of eight Centaurea L. species. Article Snippet: Canan Karamenderes*, Sibel Konyalioglu, Shabana Khan and Ikhlas A. Khan Department of Pharmaceutical Botany, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey Department of Biochemistry, Faculty of Pharmacy, Ege University, 35100 Bornova, Izmir, Turkey National Center for Natural Products Research, The University of Mississippi, University, MS 38677, USA Department of Pharmacognosy, School of Pharmacy, The University of Mississippi, University, MS 38677, USA |



