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pgl2-mdr1 (mdr1 - 137 +30 bgl ii site pgl2-basic vector  (Promega)

 
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    Structured Review

    Promega pgl2-mdr1 (mdr1 - 137 +30 bgl ii site pgl2-basic vector
    Pgl2 Mdr1 (Mdr1 137 +30 Bgl Ii Site Pgl2 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl2+basic+vector/mdr1+promoter+firefly+luciferase+plasmid+p+10224mdr/pmc02048671-49-0-20
    Average 90 stars, based on 1 article reviews
    pgl2-mdr1 (mdr1 - 137 +30 bgl ii site pgl2-basic vector - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Transfection:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Luciferase:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Plasmid Preparation:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Control:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Incubation:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Lysis:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Activation Assay:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Activity Assay:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Expressing:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Real-time Polymerase Chain Reaction:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Western Blot:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Transgenic Assay:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Mouse Assay:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Generated:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Construct:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Over Expression:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Concentration Assay:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Sequencing:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.

    Clone Assay:

    Article Title: Piperine activates human pregnane X receptor to induce the expression of cytochrome P450 3A4 and multidrug resistance protein 1
    Article Snippet: MDR1 full-length promoter construct containing 8 kb of the MDR1 promoter region (p-8055MDR) was kindly provided by Dr. Oliver Burk ( Geick et al., 2001 ) (Dr. Margarete Fischer-Bosch Institute of Clinical Pharmacology, Germany) and used to construct pGL4- MDR1 -luc in pGL4.14 (Promega, Madison, WI) following a method described previously ( Lin et al., 2008 ).

    Article Title: In Vivo Imaging of Human MDR1 Transcription in the Brain and Spine of MDR1-Luciferase Reporter Mice
    Article Snippet: Creation of MDR1-Luc Transgenic Mice The human MDR1-luciferase plasmid was generated by amplifying the human MDR1 promoter (−9,912/+180, relative to the transcription initiation site) and ligating it into the KpnI/SmaI site of pGL3Basic (Promega, Madison, WI) as described previously ( Schuetz et al., 2002 ).

    Article Title: Contribution of hepatic organic anion transporting polypeptides (OATPs) to docetaxel uptake and clearance
    Article Snippet: The blots were washed with PBS containing 0.05% Tween 20 for 15 min (3 X 5 min) and then incubated for 1h with the secondary antibody an anti-Rabbit IgG for OATP1B1 and OATP1B3 and an anti-mouse IgG antibody for MDR1 conjugated with horseradish peroxidase (HRP) (1:10,000 dilution; Promega, Madison, WI).

    Article Title: Citrus auraptene induces drug efflux transporter P-glycoprotein expression in human intestinal cells.
    Article Snippet: P-glycoprotein (encoded by MDR1) is a membrane transport protein expressed in the intestine, liver, kidney, placenta, and blood–brain barrier.. It excludes various clinically important drugs from cells, such as verapamil, digoxin, tacrolimus, and vinblastine.. Therefore, human P-glycoprotein plays important roles in drug absorption, distribution, and excretion.

    Article Title: Effects of Natural Polyphenols on the Expression of Drug Efflux Transporter P-Glycoprotein in Human Intestinal Cells
    Article Snippet: LS174T cells (1 × 10 5 cells/well) were seeded in 24-well plates and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the presence or absence of 50 ng of the vector expressing human PXR or RXRα (pSG5-hPXR or pCMX-hRXRα, respectively) using FuGENE HD transfection reagent (Promega) for 24 h. Then, the cells were incubated with 5, 10, or 20 μM polyphenols for 48 h. The cells were lysed using Promega Reporter Lysis Buffer.

    Article Title: Microtubule-targeting anticancer drug eribulin induces drug efflux transporter P-glycoprotein
    Article Snippet: LS174T or MCF7 cells were seeded in 24-well plates (1 × 10 5 cells/well) and cultured for 24 h. Then, the cells were transiently transfected with 300 ng of MDR1 promoter firefly luciferase plasmid (p-10224MDR) and 200 ng of the control HSV-TK Renilla luciferase plasmid (pGL4.74) (Promega, Madison, WI, USA) in the absence or presence of 50 ng of pEF-hPXR or pEF6/V5 (empty vector), respectively, or 200 ng of pFN21A-hSMRT using Fugene HD transfection reagent (Promega) for 24 h. Next, the cells were incubated with 0.1, 0.5, 2, or 5 μM anticancer drugs for 48 h and lysed using Promega Reporter Lysis Buffer.



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