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Promega 5× passive lysis buffer
5× Passive Lysis Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl2+basic+vector/pgl2basic/pmc02584829-73-3-19
Average 90 stars, based on 1 article reviews
5× passive lysis buffer - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Polymerase Chain Reaction:

Article Title: Elevated FANCA expression determines a worse prognosis in chronic lymphocytic leukemia and interferes with p53 function
Article Snippet: The pG13Luc p53-reporter plasmid was a kind gift from Dr. Dolores Delgado [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), Santander, Spain]. .. A genomic PCR fragment of 1001 bp (2994 to +7, referred to as the initiation of transcription) from the second promoter of TP73 was amplified with specific primers [sense (59-GAAAGCAGGCAGGTGGTTTGGG-39) and antisense (59-TGAATCCAACAACAAAACCCGC-39)] and cloned into the pGL2basic luciferase reporter vector (Promega, Madison, WI, USA). ..

Article Title: Evidence for a Lack of a Direct Transcriptional Suppression of the Iron Regulatory Peptide Hepcidin by Hypoxia-Inducible Factors
Article Snippet: .. Three different hepcidin promoter fragments were amplified from genomic DNA by PCR using the primers given in and Combizyme Polymerase Mix (Invitek), and cloned into the pGL2basic vector (Promega). ..

Amplification:

Article Title: Elevated FANCA expression determines a worse prognosis in chronic lymphocytic leukemia and interferes with p53 function
Article Snippet: The pG13Luc p53-reporter plasmid was a kind gift from Dr. Dolores Delgado [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), Santander, Spain]. .. A genomic PCR fragment of 1001 bp (2994 to +7, referred to as the initiation of transcription) from the second promoter of TP73 was amplified with specific primers [sense (59-GAAAGCAGGCAGGTGGTTTGGG-39) and antisense (59-TGAATCCAACAACAAAACCCGC-39)] and cloned into the pGL2basic luciferase reporter vector (Promega, Madison, WI, USA). ..

Article Title: Evidence for a Lack of a Direct Transcriptional Suppression of the Iron Regulatory Peptide Hepcidin by Hypoxia-Inducible Factors
Article Snippet: .. Three different hepcidin promoter fragments were amplified from genomic DNA by PCR using the primers given in and Combizyme Polymerase Mix (Invitek), and cloned into the pGL2basic vector (Promega). ..

Clone Assay:

Article Title: Elevated FANCA expression determines a worse prognosis in chronic lymphocytic leukemia and interferes with p53 function
Article Snippet: The pG13Luc p53-reporter plasmid was a kind gift from Dr. Dolores Delgado [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), Santander, Spain]. .. A genomic PCR fragment of 1001 bp (2994 to +7, referred to as the initiation of transcription) from the second promoter of TP73 was amplified with specific primers [sense (59-GAAAGCAGGCAGGTGGTTTGGG-39) and antisense (59-TGAATCCAACAACAAAACCCGC-39)] and cloned into the pGL2basic luciferase reporter vector (Promega, Madison, WI, USA). ..

Article Title: Real-time measurement of E2: ERα transcriptional activity in living cells.
Article Snippet: .. To generate the reporter plasmid pGL2Basic Neo_NLucPest_3xERE TATA containing the NLuc‐PEST gene under the control of the 3xERE TATA promoter, the NLuc gene was first excised by the pNL2.2 (Promega) using KpnI/BamHI sites and cloned into the pGL2Basic Neo 3xERE TATA (a generous gift of Dr Wilson) (Wilson, Bobseine, & Gray, 2004) using KpnI/BamHI sites. ..

Article Title: Transcriptional upregulation of MMP-9 gene under hyperglycemic conditions in AGS cells: Role of AP-1 transcription factor.
Article Snippet: Gastric cancer and diabetes are two complex and interrelated diseases having significant impact on global health.. Hyperglycemic condition notably exacerbates cancer by promoting inflammation, angiogenesis, and metastasis.. Elevated glucose levels can also upregulate the expression of specific matrix metalloproteinases (MMPs), especially MMP-9, which is associated with cancer cell migration and invasion.

Article Title: FOXO1 Couples KGF and PI-3K/AKT Signaling to NKX2.1-Regulated Differentiation of Alveolar Epithelial Cells
Article Snippet: The Nthy-ori 3-1 cell line (#90011609, Millipore, Sigma, Burlington, MA, USA) was cultured in RPMI 1640 medium that was supplemented with 2 mM glutamine and 10% FBS. .. 3.7- SFTPC -Luc contains the 3.7-kb human SFTPC promoter in pGL2Basic (Promega, Madison, WI, USA). pRC/CMV/NKX2.1 contains the 2.3-kb human NKX2.1 cDNA in pRC/CMV (Invitrogen). p318 mu- Sftpc -Luc contains −318 to -118 of the murine Sftpc promoter cloned into SmaI/XhoI sites of pGL2Basic [ ]. pCMV6-XL4/FOXO1 containing the human FOXO1 cDNA was purchased from Origene (Rockville, MD, USA). pCDNA3/FOXO1-AAA and pCDNA3/FOXO1 H215R were purchased from Addgene (Cambridge, MA, USA). pCDNA3/FOXO1, pCDNA3-FOXO1-N (1–257 a.a.), pCDNA3-FOXO1-C (211–416 a.a.), pGEX-KG-FOXO1 and pGEX-KG-FOXO1-N (1–257 a.a.) were generous gifts from Dr. K.L. ..

Article Title: Evidence for a Lack of a Direct Transcriptional Suppression of the Iron Regulatory Peptide Hepcidin by Hypoxia-Inducible Factors
Article Snippet: .. Three different hepcidin promoter fragments were amplified from genomic DNA by PCR using the primers given in and Combizyme Polymerase Mix (Invitek), and cloned into the pGL2basic vector (Promega). ..

Article Title: Tau Protein as a New Regulator of Cellular Prion Protein Transcription.
Article Snippet: .. Number AJ289875) was excised from the pPrPS2.7 plasmid, a gift from Prof. John Collinge [24], and was cloned in the correct orientation into the promoter-less luciferase vector pGL2Basic (Promega, Madison, WI, USA) to generate construct pPrP-pGL2. ..

Luciferase:

Article Title: Elevated FANCA expression determines a worse prognosis in chronic lymphocytic leukemia and interferes with p53 function
Article Snippet: The pG13Luc p53-reporter plasmid was a kind gift from Dr. Dolores Delgado [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), Santander, Spain]. .. A genomic PCR fragment of 1001 bp (2994 to +7, referred to as the initiation of transcription) from the second promoter of TP73 was amplified with specific primers [sense (59-GAAAGCAGGCAGGTGGTTTGGG-39) and antisense (59-TGAATCCAACAACAAAACCCGC-39)] and cloned into the pGL2basic luciferase reporter vector (Promega, Madison, WI, USA). ..

Article Title: Tau Protein as a New Regulator of Cellular Prion Protein Transcription.
Article Snippet: .. Number AJ289875) was excised from the pPrPS2.7 plasmid, a gift from Prof. John Collinge [24], and was cloned in the correct orientation into the promoter-less luciferase vector pGL2Basic (Promega, Madison, WI, USA) to generate construct pPrP-pGL2. ..

Plasmid Preparation:

Article Title: Elevated FANCA expression determines a worse prognosis in chronic lymphocytic leukemia and interferes with p53 function
Article Snippet: The pG13Luc p53-reporter plasmid was a kind gift from Dr. Dolores Delgado [Institute of Biomedicine and Biotechnology of Cantabria (IBBTEC), Santander, Spain]. .. A genomic PCR fragment of 1001 bp (2994 to +7, referred to as the initiation of transcription) from the second promoter of TP73 was amplified with specific primers [sense (59-GAAAGCAGGCAGGTGGTTTGGG-39) and antisense (59-TGAATCCAACAACAAAACCCGC-39)] and cloned into the pGL2basic luciferase reporter vector (Promega, Madison, WI, USA). ..

Article Title: Real-time measurement of E2: ERα transcriptional activity in living cells.
Article Snippet: .. To generate the reporter plasmid pGL2Basic Neo_NLucPest_3xERE TATA containing the NLuc‐PEST gene under the control of the 3xERE TATA promoter, the NLuc gene was first excised by the pNL2.2 (Promega) using KpnI/BamHI sites and cloned into the pGL2Basic Neo 3xERE TATA (a generous gift of Dr Wilson) (Wilson, Bobseine, & Gray, 2004) using KpnI/BamHI sites. ..

Article Title: Transcriptional upregulation of MMP-9 gene under hyperglycemic conditions in AGS cells: Role of AP-1 transcription factor.
Article Snippet: Gastric cancer and diabetes are two complex and interrelated diseases having significant impact on global health.. Hyperglycemic condition notably exacerbates cancer by promoting inflammation, angiogenesis, and metastasis.. Elevated glucose levels can also upregulate the expression of specific matrix metalloproteinases (MMPs), especially MMP-9, which is associated with cancer cell migration and invasion.

Article Title: Evidence for a Lack of a Direct Transcriptional Suppression of the Iron Regulatory Peptide Hepcidin by Hypoxia-Inducible Factors
Article Snippet: .. Three different hepcidin promoter fragments were amplified from genomic DNA by PCR using the primers given in and Combizyme Polymerase Mix (Invitek), and cloned into the pGL2basic vector (Promega). ..

Article Title: Tau Protein as a New Regulator of Cellular Prion Protein Transcription.
Article Snippet: .. Number AJ289875) was excised from the pPrPS2.7 plasmid, a gift from Prof. John Collinge [24], and was cloned in the correct orientation into the promoter-less luciferase vector pGL2Basic (Promega, Madison, WI, USA) to generate construct pPrP-pGL2. ..

Control:

Article Title: Real-time measurement of E2: ERα transcriptional activity in living cells.
Article Snippet: .. To generate the reporter plasmid pGL2Basic Neo_NLucPest_3xERE TATA containing the NLuc‐PEST gene under the control of the 3xERE TATA promoter, the NLuc gene was first excised by the pNL2.2 (Promega) using KpnI/BamHI sites and cloned into the pGL2Basic Neo 3xERE TATA (a generous gift of Dr Wilson) (Wilson, Bobseine, & Gray, 2004) using KpnI/BamHI sites. ..

Expressing:

Article Title: Transcriptional upregulation of MMP-9 gene under hyperglycemic conditions in AGS cells: Role of AP-1 transcription factor.
Article Snippet: Gastric cancer and diabetes are two complex and interrelated diseases having significant impact on global health.. Hyperglycemic condition notably exacerbates cancer by promoting inflammation, angiogenesis, and metastasis.. Elevated glucose levels can also upregulate the expression of specific matrix metalloproteinases (MMPs), especially MMP-9, which is associated with cancer cell migration and invasion.

Generated:

Article Title: Transcriptional upregulation of MMP-9 gene under hyperglycemic conditions in AGS cells: Role of AP-1 transcription factor.
Article Snippet: Gastric cancer and diabetes are two complex and interrelated diseases having significant impact on global health.. Hyperglycemic condition notably exacerbates cancer by promoting inflammation, angiogenesis, and metastasis.. Elevated glucose levels can also upregulate the expression of specific matrix metalloproteinases (MMPs), especially MMP-9, which is associated with cancer cell migration and invasion.

other:

Article Title: Transcriptional activity of the long control region in human papillomavirus type 33 intratype variants.
Article Snippet: Representative HPV 33 LCR sequence variants were directionally cloned into the promoterless luciferase reporter vector pGL2-Basic carrying the firefly (Photinus pyralis) luciferase gene (Promega, Madison, Wisconsin, USA), as previously described [32].

Construct:

Article Title: Tau Protein as a New Regulator of Cellular Prion Protein Transcription.
Article Snippet: .. Number AJ289875) was excised from the pPrPS2.7 plasmid, a gift from Prof. John Collinge [24], and was cloned in the correct orientation into the promoter-less luciferase vector pGL2Basic (Promega, Madison, WI, USA) to generate construct pPrP-pGL2. ..



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