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Promega pgl 2 basic vectors
Pgl 2 Basic Vectors, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl-2+basic/pgl+basic/pmc03323005-230-8-9
Average 90 stars, based on 1 article reviews
pgl 2 basic vectors - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Transfection:

Article Title: Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity
Article Snippet: .. Transfections of promoter constructs in pGL Basic (with firefly luciferase activity) were performed using FuGENE HD transfection reagent (Promega), as per manufacturer’s instructions. .. To control for variation in transfection efficiency among replicates, promoter constructs were co-transfected with the Renilla luciferase vector, pRL-TK (Promega).

Construct:

Article Title: Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity
Article Snippet: .. Transfections of promoter constructs in pGL Basic (with firefly luciferase activity) were performed using FuGENE HD transfection reagent (Promega), as per manufacturer’s instructions. .. To control for variation in transfection efficiency among replicates, promoter constructs were co-transfected with the Renilla luciferase vector, pRL-TK (Promega).

Luciferase:

Article Title: Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity
Article Snippet: .. Transfections of promoter constructs in pGL Basic (with firefly luciferase activity) were performed using FuGENE HD transfection reagent (Promega), as per manufacturer’s instructions. .. To control for variation in transfection efficiency among replicates, promoter constructs were co-transfected with the Renilla luciferase vector, pRL-TK (Promega).

Activity Assay:

Article Title: Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity
Article Snippet: .. Transfections of promoter constructs in pGL Basic (with firefly luciferase activity) were performed using FuGENE HD transfection reagent (Promega), as per manufacturer’s instructions. .. To control for variation in transfection efficiency among replicates, promoter constructs were co-transfected with the Renilla luciferase vector, pRL-TK (Promega).

other:

Article Title: Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth
Article Snippet: Then, promoters were cut from T-promoter vectors by KpnI/SalI, which were inserted before the luciferase sequence of pGL-basic (Promega, Madison,WI, USA) using KpnI/XhoI, constructing promoter-luciferase expression vectors.

Article Title: Effective Cryopreservation of a Bioluminescent Auxotrophic Escherichia coli -Based Amino Acid Array to Enable Long-Term Ready-to-Use Applications
Article Snippet: The luc component was amplified using the pGL-Basic vector (Promega, WI, USA) and the PCR products were cloned into the pTAC to produce pTAC-luc.

Article Title: KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.
Article Snippet: The 5’flanking region (2000bp) of KIF2A was amplified from human genomic and inserted into the multi-cloning site of pGL-3.0-Basic (Promega, Madison, WI, USA) to generate reporter plasmids.

Article Title: Malignant tumor cell suppressor protein, malignant tumor cell suppressor gene, maligant tumor cell suppressive viral vector, and kit using the same
Article Snippet: Subsequently, a vector pGL3328 which is constructed by being inserted the hTERT promoter into the restriction enzymes sites Mlu I and Bgl II of the pGL-basic (manufactured by Promega) (the vector pGL3328 provided by Dr. Satoru Kyo, Graduate School of Medical Science, Kanazawa University; see, for example, Takakura et al., Cloning of human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells.

Article Title: LRP16 Integrates into NF-κB Transcriptional Complex and Is Required for Its Functional Activation
Article Snippet: GAL4-luc was prepared by cloning five GAL4 binding sites into the pGL-Basic vector (Promega).

Article Title: Possible roles of the transcription factor Nrf1 (NFE2L1) in neural homeostasis by regulating the gene expression of deubiquitinating enzymes.
Article Snippet: Accepted Manuscript Possible roles of the transcription factor Nrf1 (NFE2L1) in neural homeostasis by regulating the gene expression of deubiquitinating enzymes Hiroaki Taniguchi, Shota Okamuro, Misaki Kohji, Tsuyoshi Waku, Kaori Kubo, Atsushi Hatanaka, Yimeng Sun, A.M. Masudul Azad Chowdhury, Akiyoshi Fukamizu, Akira Kobayashi PII: S0006-291X(17)30047-5 DOI: 10.1016/j.bbrc.2017.01.038 Reference: YBBRC 37100 To appear in: Biochemical and Biophysical Research Communications Received Date: 30 December 2016 Accepted Date: 9 January 2017 Please cite this article as: H. Taniguchi, S. Okamuro, M. Kohji, T. Waku, K. Kubo, A. Hatanaka, Y.. Sun, A.M.M.A.. Chowdhury, A. Fukamizu, A. Kobayashi, Possible roles of the transcription factor Nrf1 (NFE2L1) in neural homeostasis by regulating the gene expression of deubiquitinating enzymes, Biochemical and Biophysical Research Communications (2017), doi: 10.1016/j.bbrc.2017.01.038.

Amplification:

Article Title: ANRIL Promoter DNA Methylation: A Perinatal Marker for Later Adiposity
Article Snippet: .. The first set amplified the ANRIL promoter (− 926 to + 20 relative to ANRIL TSS); a Hin dIII restriction site was added to the forward primer, and an Nco I restriction site to the reverse primer for cloning into pGL Basic (Promega, UK) to create pGL − 951. ..

Cloning:

Article Title: ANRIL Promoter DNA Methylation: A Perinatal Marker for Later Adiposity
Article Snippet: .. The first set amplified the ANRIL promoter (− 926 to + 20 relative to ANRIL TSS); a Hin dIII restriction site was added to the forward primer, and an Nco I restriction site to the reverse primer for cloning into pGL Basic (Promega, UK) to create pGL − 951. ..



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