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pgl-3-basic vector  (Promega)

 
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    Promega pgl-3-basic vector
    Pgl 3 Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3-basic+vector/e1751/pmc07642041-79-36-39
    Average 90 stars, based on 1 article reviews
    pgl-3-basic vector - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Activator of KAT3 histone acetyltransferase family ameliorates a neurodevelopmental disorder phenotype in the syntaxin 1A ablated mouse model.
    Article Snippet: Cg-Stx1a knockout mice This paper RBRC Acc# RBRC10236 Mouse: C57BL/6J, male, 2–3 months old Inbred (JNCASR) N/A Mouse: C57BL/6J The Jacksons Laboratory RRID:IMSR_JAX:008890 Oligonucleotides Oligonucleotides for EMSA and competition assays (See method details) This paper Table S3 Primers for ChIP assay (See method details) This paper Table S4 Recombinant DNA P300 short hairpin RNA plasmid Santa Cruz Biotechnology Cat# sc-29431-SH Negative control short hairpin RNA plasmid Santa Cruz Biotechnology Cat# sc-108060 pGL3-TK Promega Cat# E1751 phRG-TK Promega Cat# E6291 Software and algorithms Proteome Discoverer 1.4 Thermo Fisher Scientific https://tools.thermofisher.com/ content/sfs/manuals/ManXCALI-97506-Proteome-Discoverer14-User-ManXCALI97506-A-EN.pdf Multi Gauge 3 FujiFilm https://www.fujifilm.com/us/en Powerchrom ES280 eDAQ https://www.edaq.com/ES280 EZR1.61 Kanda Y https://www.nature.com/articles/ bmt2012244.pdf GraphPad Prism 4.03 GraphPad https://www.graphpad.com/features Other Cryotome CM3050S Leica Model# CM3050S Field Emission Scanning Electron Microscope (FESEM) Thermo Fischer (FEI) Model# APREO 2 S (Continued on next page) 14 Cell Reports 43, 114101, April 23, 2024

    Article Title: Free fatty acid receptor 4 deletion attenuates colitis by modulating Treg Cells via ZBED6-IL33 pathway
    Article Snippet: A 2100 bp human IL33 promoter sequence was cloned into the pGL30-basic vector with a firefly luciferase (LUC) reporter gene (Promega, E1751), generating pGL3- IL33 .


    Article Title: CircRNA_0040705 promotes the progression of hepatocellular carcinoma.
    Article Snippet: Correspondence Shen Lei, Department of Gastroenterology, Renmin Hospital of Wuhan University, Zhangzhidong Road, Wuchang District, Wuhan, Hubei 430060, China.. Email: leishenwuhan@126.com Abstract Circular RNAs (circRNAs) are involved in cancer progression.. Nonetheless, the role and mechanism of circ_0040705 in hepatocellular carcinoma (HCC) are unclear.

    Reporter Gene Assay:

    Article Title: MiR-484 promotes the malignant progression of glioma by inhibiting CDKN2A expression.
    Article Snippet: .. Dual-luciferase reporter gene assay NCBI (https://www.ncbi.nlm.nih.gov/) provided the 3’-UTR sequence of CDKN2A, and then the 3’-UTR sequence was connected with the pmirGLO luciferase vector (E1751, Promega, USA) to conduct the pmirGLOCDKN2A-3’-UTR recombinant. ..

    Sequencing:

    Article Title: MiR-484 promotes the malignant progression of glioma by inhibiting CDKN2A expression.
    Article Snippet: .. Dual-luciferase reporter gene assay NCBI (https://www.ncbi.nlm.nih.gov/) provided the 3’-UTR sequence of CDKN2A, and then the 3’-UTR sequence was connected with the pmirGLO luciferase vector (E1751, Promega, USA) to conduct the pmirGLOCDKN2A-3’-UTR recombinant. ..

    Luciferase:

    Article Title: MiR-484 promotes the malignant progression of glioma by inhibiting CDKN2A expression.
    Article Snippet: .. Dual-luciferase reporter gene assay NCBI (https://www.ncbi.nlm.nih.gov/) provided the 3’-UTR sequence of CDKN2A, and then the 3’-UTR sequence was connected with the pmirGLO luciferase vector (E1751, Promega, USA) to conduct the pmirGLOCDKN2A-3’-UTR recombinant. ..

    Article Title: A distal convoluted tubule‐specific isoform of murine SLC41A3 extrudes magnesium
    Article Snippet: The p.D204A variant in SLC41A3‐Iso 2 and hSLC41A3‐Iso 4 was generated using Q5 Site‐Directed Mutagenesis (New England BioLabs, E0554) following manufacturer's instructions. .. Commercially available plasmids encoding Firefly luciferase (Promega, E1751) and Renilla luciferase (Promega, E2261) were used for the luciferase assay. ..

    Recombinant:

    Article Title: MiR-484 promotes the malignant progression of glioma by inhibiting CDKN2A expression.
    Article Snippet: .. Dual-luciferase reporter gene assay NCBI (https://www.ncbi.nlm.nih.gov/) provided the 3’-UTR sequence of CDKN2A, and then the 3’-UTR sequence was connected with the pmirGLO luciferase vector (E1751, Promega, USA) to conduct the pmirGLOCDKN2A-3’-UTR recombinant. ..

    Plasmid Preparation:

    Article Title: A methionine-Mettl3- N 6 -methyladenosine axis promotes polycystic kidney disease
    Article Snippet: Plasmid - pLightSwitch Empty (pLS) 3′-UTR , Switchgear Genomics , S890005. .. Plasmid - pGL3 , Promega , E1751. ..



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    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
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    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
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    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
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    pgl-3-basic vector  (Promega)
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    Promega pgl-3-basic vector
    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
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    pgl-3 luciferase reporter basic vector  (Promega)
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    Promega pgl-3 luciferase reporter basic vector
    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
    Pgl 3 Luciferase Reporter Basic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3-basic+vector/pgl3+basic/ppr0123714-41-34-38
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    pgl-3 basic recombinant vector containing p53-re1 trim8 gene  (Promega)
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    Promega pgl-3 basic recombinant vector containing p53-re1 trim8 gene
    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the <t>pGL</t> <t>3</t> -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).
    Pgl 3 Basic Recombinant Vector Containing P53 Re1 Trim8 Gene, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pgl+3-basic+vector/pgl3+basic/pmc06856647-78-19-54
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Journal: PLoS ONE

    Article Title: p21-Activated Kinase 3 (PAK3) Is an AP-1 Regulated Gene Contributing to Actin Organisation and Migration of Transformed Fibroblasts

    doi: 10.1371/journal.pone.0066892

    Figure Lengend Snippet: The (−2436/+149) region of the PAK3 promoter, containing several putative cJun/AP- binding sites, was cloned into the pGL 3 -Basic reporter vector. A: Luciferase promoter reporter assays performed in control cells, Rat1a-GFP, and Rat1a-J4 cells transiently transfected with empty vector, pGL 3 -Basic, and PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). Cells were grown in the absence and presence of doxycycline. B: Luciferase promoter reporter assays performed in the parental rat fibroblast cell line, Rat1a, transfected with either pCMV-cJun or empty pCMV vector with the PAK3 promoter construct containing vector, pGL 3 -Basic-pPAK3 (−2436/+149). A plasmid containing four AP-1 binding sites, 4 X AP-1-Luc, was used as a control showing cJun activation. C: Luciferase reporter promoter assays for deletion constructs of the PAK3 promoter region (−2436/+149), (−2329/+149), (−684/+149) and (−179/+149) as well as a (−179/+149) with a mutated cJun/AP-1 binding site at position (+52/+60), in the presence and absence of doxycycline-induced cJun/AP-1 expression. D: Luciferase reporter assays, using the (−179/+149) PAK3 promoter construct, showing the effect of transient cJun inhibition on PAK3 promoter activity in the absence and presence of doxycycline induced cJun/AP-1 over-expression. Results show the mean±S.E. of experiments performed in triplicate and repeated at least three times. (*p≤0.05 and **p≤0.01).

    Article Snippet: PCR using high fidelity Expand Plus DNA Polymerase (Roche) was performed on rat genomic DNA, the products were sub-cloned into the pGEM-T Easy vector (Promega) and excised for cloning into the luciferase reported vector, pGL 3 -Basic (Promega) using Mlu1 and Xho1 restriction enzymes.

    Techniques: Binding Assay, Clone Assay, Plasmid Preparation, Luciferase, Transfection, Construct, Activation Assay, Expressing, Inhibition, Activity Assay, Over Expression