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Addgene inc pll 3 7
Pll 3 7, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/personalarrayer16+microarray+spotter/pLL3%2E7+(Plasmid+%2311795)/pmc06631631-47-31-33
Average 95 stars, based on 359 article reviews
pll 3 7 - by Bioz Stars, 2026-11
95/100 stars

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Related Articles

Knockdown:

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGA AAC AAG TGA AAG CAT A) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress.
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGAAACAAGTGAAAGCATA) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Construct:

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGA AAC AAG TGA AAG CAT A) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress.
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGAAACAAGTGAAAGCATA) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: The focal adhesion kinases regulate leptin action and the weight reducing effect of HDAC6 inhibition.
Article Snippet: The FLAG-tagged human leptin receptorORFwaspurchased from Sino Biologicals (Beijing, China). .. Scramble shRNA construct in pLKO.1 vectors was subcloned into pLL3.7 (#11795, Addgene), which also encodes GFP. ..

shRNA:

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGA AAC AAG TGA AAG CAT A) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress.
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGAAACAAGTGAAAGCATA) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: The focal adhesion kinases regulate leptin action and the weight reducing effect of HDAC6 inhibition.
Article Snippet: The FLAG-tagged human leptin receptorORFwaspurchased from Sino Biologicals (Beijing, China). .. Scramble shRNA construct in pLKO.1 vectors was subcloned into pLL3.7 (#11795, Addgene), which also encodes GFP. ..

Clone Assay:

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGA AAC AAG TGA AAG CAT A) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress.
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGAAACAAGTGAAAGCATA) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Plasmid Preparation:

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGA AAC AAG TGA AAG CAT A) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

Article Title: Highly networked immunogen composition
Article Snippet: .. Non-limiting examples of lentiviral vectors include pLVX-EF1alpha-AcGFP1-C1 (Clontech Catalog #631984), pLVX-EF1alpha-IRES-mCherry (Clontech Catalog #631987), pLVX-Puro (Clontech Catalog #632159), pLVX-IRES-Puro (Clontech Catalog #632186), pLenti6/V5-DESTTM (Thermo Fisher), pLenti6.2/V5-DESTTM (Thermo Fisher), pLKO.1 (Plasmid #10878 at Addgene), pLKO.3G (Plasmid #14748 at Addgene), pSico (Plasmid #11578 at Addgene), pLJM1-EGFP (Plasmid #19319 at Addgene), FUGW (Plasmid #14883 at Addgene), pLVTHM (Plasmid #12247 at Addgene), pLVUT-tTR-KRAB (Plasmid #11651 at Addgene), pLL3.7 (Plasmid #11795 at Addgene), pLB (Plasmid #11619 at Addgene), pWPXL (Plasmid #12257 at Addgene), pWPI (Plasmid #12254 at Addgene), EF.CMV.RFP (Plasmid #17619 at Addgene), pLenti CMV Puro DEST (Plasmid #17452 at Addgene), pLenti-puro (Plasmid #39481 at Addgene), pULTRA (Plasmid #24129 at Addgene), pLX301 (Plasmid #25895 at Addgene), pHIV-EGFP (Plasmid #21373 at Addgene), pLV-mCherry (Plasmid #36084 at Addgene), pLionII (Plasmid #1730 at Addgene), pInducer10-mir-RUP-PheS (Plasmid #44011 at Addgene). ..

Article Title: The Notch1 intracellular domain orchestrates mechanotransduction of fluid shear stress.
Article Snippet: DNA fragments were assembled using NEBuilder HiFi DNA Assembly Master Mix. .. For knockdown-rescue constructs, a shRNA targeting the 3′ UTR of NOTCH1 (GGAAACAAGTGAAAGCATA) was cloned into the HpaI and XhoI sites of pLL3.7 (plasmid #11795; Addgene). .. Either full-length Notch1 (Notch1-WT) or Notch1 truncated at Arg1762 (Notch1-ΔICD) fused on the C terminus with mEmerald (plasmid # 54025; Addgene) was cloned after the CMV promoter in pLL3.7 using AgeI and EcoRI.

other:

Article Title: The focal adhesion kinases regulate leptin action and the weight reducing effect of HDAC6 inhibition
Article Snippet: The wild-type Fak (#50515), autophosphorylation-site mutant FAK Y397F (#50516), FLAG-tagged Stat3 , and LacZ constructs were obtained from Addgene (Cambridge, MA).



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