permeabilization buffer kit i (R&D Systems)
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permeabilization buffer kit i
Permeabilization Buffer Kit I, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+kit/Flow+Cytometry+Fixation+%26+Permeabilization+Buffer+Kit+I/pmc12901808-68-13-17
Average 94 stars, based on 65 article reviews
Permeabilization Buffer Kit I, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer+kit/Flow+Cytometry+Fixation+%26+Permeabilization+Buffer+Kit+I/pmc12901808-68-13-17
Average 94 stars, based on 65 article reviews
permeabilization buffer kit i - by Bioz Stars,
2026-09
94/100 stars
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Staining:Article Title: Unaltered NKG2D-CAR T cell function under hypoxia in osteosarcoma in vitro. Article Snippet: OS cell lines and CAR T cells were stained with combinations of fluorochrome-labeled anti-human antibodies against CD276 (B7-H3)-FITC (Clone REA1094; Miltenyi Biotec), CD73-APC (Clone AD2, Biolegend), CD274 (PD-L1)FITC (Clone MIH2, Biolegend), HMGB1/HMG-1-PE (Clone IC1690P, R&D Systems), Galectin-9-APC (Clone 9M1-3, Biolegend), CD270 (HVEM)-APC (Clone 122, Biolegend), CD40-FITC (Clone 5C3, Biolegend), CD252 (OX40L)-PE (Clone 11C3.1, Biolegend), CD137L (4-1BB Ligand)-APC (Clone 5F4, Biolegend), MICA-APC (Clone 159,227, R&D Systems), MICB- APC (Clone 236,511, R&D Systems), UBLP-1- Alexa Fluor 488 (Clone 170,818, R&D Systems), ULBP-2/5/6-PE (Clone 165,903, R&D Systems), ULBP-3-PE (Clone 166,510, R&D Systems), ULBP4-APC (Clone 709,116, R&D Systems), NKG2D-PEVio770 (Clone REA797, Miltenyi Biotec), CD25-PEVio770 (Clone REA945, Miltenyi Biotec), PD1-PEVio770 (Clone REA1165, Miltenyi Biotec), NTBA-PE (Clone NT7, Biolegend), TIM-3-FITC (Clone F38-2E2, Biolegend), CD107aPE (Clone H4A3, Biolegend), CD69-PECy7 (Clone FN50, Biolegend). .. Intracellular staining was performed using GranzB-Pacific Blue (Clone GB11, Biolegend) and Fixation and Article Title: IKKε-deficient macrophages impede cardiac repair after myocardial infarction by enhancing the macrophage-myofibroblast transition. Article Snippet: .. Cell surface molecules were simultaneously stained with fluorophoreconjugated antibodies at room temperature for 30min, and intracellular molecules were detected using a Fixation and Article Title: Biodegradable Electrospun PLGA Nanofibers-Encapsulated Trichinella Spiralis Antigens Protect from Relapsing Experimental Autoimmune Encephalomyelitis and Related Gut Microbiota Dysbiosis Article Snippet: .. After the surface staining, the cells were fixed and permeabilised using the Article Title: Trichinella spiralis extracellular vesicles induce anti-inflammatory and regulatory immune responses in vitro. Article Snippet: The helminth Trichinella spiralis, through its excretory-secretory (ES L1) products, induces immune regulatory mechanisms that modulate the host's immune response not only to itself, but also to bystander antigens, foreign or self in origin, which can result in the alleviation of inflammatory diseases.. Under the influence of ES L1, dendritic cells (DCs) acquire a tolerogenic phenotype and the capacity to induce Th2 and regulatory responses.. Since ES L1 products represent a complex mixture of proteins and extracellular vesicles (TsEVs) the aim of this study was to investigate the impact of TsEVs, isolated from ES L1 products, on phenotypic and functional characteristics of DCs and to elucidate whether TsEVs could reproduce the immunomodulatory effects of the complete ES L1 product. Article Title: Unaltered NKG2D-CAR T cell function under hypoxia in osteosarcoma in vitro Article Snippet: OS cell lines and CAR T cells were stained with combinations of fluorochrome-labeled anti-human antibodies against CD276 (B7-H3)-FITC (Clone REA1094; Miltenyi Biotec), CD73-APC (Clone AD2, Biolegend), CD274 (PD-L1)-FITC (Clone MIH2, Biolegend), HMGB1/HMG-1-PE (Clone IC1690P, R&D Systems), Galectin-9-APC (Clone 9M1-3, Biolegend), CD270 (HVEM)-APC (Clone 122, Biolegend), CD40-FITC (Clone 5C3, Biolegend), CD252 (OX40L)-PE (Clone 11C3.1, Biolegend), CD137L (4-1BB Ligand)-APC (Clone 5F4, Biolegend), MICA-APC (Clone 159,227, R&D Systems), MICB- APC (Clone 236,511, R&D Systems), UBLP-1- Alexa Fluor 488 (Clone 170,818, R&D Systems), ULBP-2/5/6-PE (Clone 165,903, R&D Systems), ULBP-3-PE (Clone 166,510, R&D Systems), ULBP-4-APC (Clone 709,116, R&D Systems), NKG2D-PEVio770 (Clone REA797, Miltenyi Biotec), CD25-PEVio770 (Clone REA945, Miltenyi Biotec), PD1-PEVio770 (Clone REA1165, Miltenyi Biotec), NTBA-PE (Clone NT7, Biolegend), TIM-3-FITC (Clone F38-2E2, Biolegend), CD107a-PE (Clone H4A3, Biolegend), CD69-PECy7 (Clone FN50, Biolegend). .. Intracellular staining was performed using GranzB-Pacific Blue (Clone GB11, Biolegend) and Fixation and Article Title: Trichinella spiralis -derived extracellular vesicles induce regulatory T cells and reduce airway allergy in mice Article Snippet: Cells were stained with surface markers for 30 minutes at +4°C with the following monoclonal antibodies (mAbs) at dilutions recommended by the manufacturer: anti-Ly6G-Alexa Fluor 488(AF488) (RB-68C5) (Invitrogen Thermo Fisher Dreieich, Germany), an-ti-CD3-Fluorescein Isothiocyanate (FITC) (17A2) BioLegend, Basel, Switzerland, an-ti-CD4-FITC (GK1.5) (Sony Biotechnology, San Jose, CA, USA), anti-MHC class II (I-A)-Phycoerythrin (PE) (NIMR-4) (eBioscience, San Diego, CA, USA), anti-CD19-PE (QA17A27), anti-CD45-PE-Dazzle 594 (30-F11), anti-Ly6C-Peridinin Chlorophyll Pro-tein/Cyanine5.5 (PerCP/Cy5.5) (all BioLegend), anti-CD8a-Peridinin Cyanine 5 (PerCy5) (53-6.7), anti-CD11b-Peridinin Cyanine 7 (PerCy7) (M1/70) (both Invitrogen), anti-TCRβ-PerCy7 (H57-597), anti-CD103-Allophycocyanin (APC)(2E7) (both BioLegend), anti-CD49b-APC (DX5) (MACS Miltenyi Biotec, Cologne, Germany), anti-CD107 (Sig-lecF)-Alexa Fluor 700 (AF700) (1RNM44N) (Invitrogen), anti-CD11c-Allophycocyanin/Cyanine7 (APC-Cy7) (N418) (BioLegend). .. Intracellular staining of cells was conducted after the surface staining, by using the flow cytometry fixation and Article Title: IKKε-deficient macrophages impede cardiac repair after myocardial infarction by enhancing the macrophage–myofibroblast transition Article Snippet: .. Cell surface molecules were simultaneously stained with fluorophore-conjugated antibodies at room temperature for 30 min, and intracellular molecules were detected using a Fixation and Article Title: Mer receptor expression promotes multiple myeloma disease development via a cell-extrinsic mechanism. Article Snippet: .. Staining for Galectin 9 was similarly assessed with the following modifications: staining was carried out using eBioscienceTM intracellular fixation and Flow Cytometry:Article Title: Trichinella spiralis extracellular vesicles induce anti-inflammatory and regulatory immune responses in vitro. Article Snippet: The helminth Trichinella spiralis, through its excretory-secretory (ES L1) products, induces immune regulatory mechanisms that modulate the host's immune response not only to itself, but also to bystander antigens, foreign or self in origin, which can result in the alleviation of inflammatory diseases.. Under the influence of ES L1, dendritic cells (DCs) acquire a tolerogenic phenotype and the capacity to induce Th2 and regulatory responses.. Since ES L1 products represent a complex mixture of proteins and extracellular vesicles (TsEVs) the aim of this study was to investigate the impact of TsEVs, isolated from ES L1 products, on phenotypic and functional characteristics of DCs and to elucidate whether TsEVs could reproduce the immunomodulatory effects of the complete ES L1 product. Article Title: Trichinella spiralis -derived extracellular vesicles induce regulatory T cells and reduce airway allergy in mice Article Snippet: Cells were stained with surface markers for 30 minutes at +4°C with the following monoclonal antibodies (mAbs) at dilutions recommended by the manufacturer: anti-Ly6G-Alexa Fluor 488(AF488) (RB-68C5) (Invitrogen Thermo Fisher Dreieich, Germany), an-ti-CD3-Fluorescein Isothiocyanate (FITC) (17A2) BioLegend, Basel, Switzerland, an-ti-CD4-FITC (GK1.5) (Sony Biotechnology, San Jose, CA, USA), anti-MHC class II (I-A)-Phycoerythrin (PE) (NIMR-4) (eBioscience, San Diego, CA, USA), anti-CD19-PE (QA17A27), anti-CD45-PE-Dazzle 594 (30-F11), anti-Ly6C-Peridinin Chlorophyll Pro-tein/Cyanine5.5 (PerCP/Cy5.5) (all BioLegend), anti-CD8a-Peridinin Cyanine 5 (PerCy5) (53-6.7), anti-CD11b-Peridinin Cyanine 7 (PerCy7) (M1/70) (both Invitrogen), anti-TCRβ-PerCy7 (H57-597), anti-CD103-Allophycocyanin (APC)(2E7) (both BioLegend), anti-CD49b-APC (DX5) (MACS Miltenyi Biotec, Cologne, Germany), anti-CD107 (Sig-lecF)-Alexa Fluor 700 (AF700) (1RNM44N) (Invitrogen), anti-CD11c-Allophycocyanin/Cyanine7 (APC-Cy7) (N418) (BioLegend). .. Intracellular staining of cells was conducted after the surface staining, by using the flow cytometry fixation and |