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PEPperPRINT gmbh pepperchip custom peptide microarray
Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with <t>microarray-based</t> linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).
Pepperchip Custom Peptide Microarray, supplied by PEPperPRINT gmbh, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/peptide+microarray/pepperchip+peptide+microarrays/pmc12119686-100-18-22
Average 90 stars, based on 1 article reviews
pepperchip custom peptide microarray - by Bioz Stars, 2026-10
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1) Product Images from "Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation"

Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2025.1578372

Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).
Figure Legend Snippet: Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).

Techniques Used: Microarray

Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).
Figure Legend Snippet: Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).

Techniques Used: Microarray, Sequencing, Activity Assay

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Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation
Article Snippet: .. The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany). ..

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Article Snippet: Several barriers for the development of an HCV vaccine still exist, including the genetic diversity of the virus, and the shortage of assessable models for in vitro and in vivo assays.. Therefore, in this study, HCV epitope mapping was performed for 59 polyprotein sequences from 7 HCV genotypes.. Around 2,880 peptides were considered epitopes for CD8+ T cells.

other:

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Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

Article Title: Naturally acquired IgG responses to Plasmodium falciparum do not target the conserved termini of the malaria vaccine candidate merozoite surface protein 2
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Article Snippet: We constructed a custom PEPperCHIP® Peptide Microarray (PEPperPRINT) containing 128, 13-amino acid (13-mer) peptides present in a collection of 494 MSP2 variants sequenced previously using CCS (34).

Sequencing:

Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation
Article Snippet: .. The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany). ..

In Vitro:

Article Title: HCV immunodominant peptide mapping reveals unique HLA-A*02-restricted signatures: insights for CD8 + T-cell-based vaccines and immunotherapies.
Article Snippet: Several barriers for the development of an HCV vaccine still exist, including the genetic diversity of the virus, and the shortage of assessable models for in vitro and in vivo assays.. Therefore, in this study, HCV epitope mapping was performed for 59 polyprotein sequences from 7 HCV genotypes.. Around 2,880 peptides were considered epitopes for CD8+ T cells.

Derivative Assay:

Article Title: HCV immunodominant peptide mapping reveals unique HLA-A*02-restricted signatures: insights for CD8 + T-cell-based vaccines and immunotherapies.
Article Snippet: Several barriers for the development of an HCV vaccine still exist, including the genetic diversity of the virus, and the shortage of assessable models for in vitro and in vivo assays.. Therefore, in this study, HCV epitope mapping was performed for 59 polyprotein sequences from 7 HCV genotypes.. Around 2,880 peptides were considered epitopes for CD8+ T cells.

Recombinant:

Article Title: HCV immunodominant peptide mapping reveals unique HLA-A*02-restricted signatures: insights for CD8 + T-cell-based vaccines and immunotherapies.
Article Snippet: Several barriers for the development of an HCV vaccine still exist, including the genetic diversity of the virus, and the shortage of assessable models for in vitro and in vivo assays.. Therefore, in this study, HCV epitope mapping was performed for 59 polyprotein sequences from 7 HCV genotypes.. Around 2,880 peptides were considered epitopes for CD8+ T cells.



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Image Search Results


Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).

Journal: Frontiers in Immunology

Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

doi: 10.3389/fimmu.2025.1578372

Figure Lengend Snippet: Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).

Article Snippet: The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany).

Techniques: Microarray

Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).

Journal: Frontiers in Immunology

Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

doi: 10.3389/fimmu.2025.1578372

Figure Lengend Snippet: Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).

Article Snippet: The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany).

Techniques: Microarray, Sequencing, Activity Assay

A Schematic model showing the PTK PamChip peptide microarray system and experimental design. B Heat map of up-and down-regulated Tyrosine (PTK assay) bait peptide phosphorylation of lorlatinib (4 h) versus DMSO treatment by peptide chip array ( n = 3 biological replicates) in H3122 and H2228 cell lines. Significant peptides ( p < 0.05) were clustered using hierarchical order and Euclidean/ward algorithm. C , D Volcano plots of the bait peptide phosphorylation from PTK chip arrays in A showing significant up- (Red) and downregulation (blue) of peptide phosphorylation as defined by ANOVA and post-hoc Dunnett’s test versus DMSO treatment ( p < 0.05). Non-significant peptides were highlighted in grey. Downregulated peptides were highlighted in light blue. ERBB family and AKT1 phosphopeptides were highlighted in green and black bold colours, respectively. E Venn diagram was generated to compare the phosphorylated Tyr peptides (up- and downregulated) after a 4 h treatment with lorlatinib in H3122 and H2228 cell lines. Tables of the specific phosphorylated Tyr sites and the associated proteins are shown. The down-regulated proteins were highlighted in cyan colour. The upregulated proteins selected for further analysis were highlighted in bold with green and black colours.

Journal: Cell Death & Disease

Article Title: Targeting ERBB3 and AKT to overcome adaptive resistance in EML4-ALK-driven non-small cell lung cancer

doi: 10.1038/s41419-024-07272-7

Figure Lengend Snippet: A Schematic model showing the PTK PamChip peptide microarray system and experimental design. B Heat map of up-and down-regulated Tyrosine (PTK assay) bait peptide phosphorylation of lorlatinib (4 h) versus DMSO treatment by peptide chip array ( n = 3 biological replicates) in H3122 and H2228 cell lines. Significant peptides ( p < 0.05) were clustered using hierarchical order and Euclidean/ward algorithm. C , D Volcano plots of the bait peptide phosphorylation from PTK chip arrays in A showing significant up- (Red) and downregulation (blue) of peptide phosphorylation as defined by ANOVA and post-hoc Dunnett’s test versus DMSO treatment ( p < 0.05). Non-significant peptides were highlighted in grey. Downregulated peptides were highlighted in light blue. ERBB family and AKT1 phosphopeptides were highlighted in green and black bold colours, respectively. E Venn diagram was generated to compare the phosphorylated Tyr peptides (up- and downregulated) after a 4 h treatment with lorlatinib in H3122 and H2228 cell lines. Tables of the specific phosphorylated Tyr sites and the associated proteins are shown. The down-regulated proteins were highlighted in cyan colour. The upregulated proteins selected for further analysis were highlighted in bold with green and black colours.

Article Snippet: To identify adaptive response mechanisms, we examined the landscape of tyrosine phosphorylation upon acute LOR treatment in EML4-ALK-positive NSCLC cell lines using PamGene microarray peptide assay, known as protein tyrosine kinase (PTK) (Fig. ).

Techniques: Peptide Microarray, Generated