peptide microarray–based screening for autoantibodies against epitopes of pre-defined 168 different antigens  (PEPperPRINT gmbh)

Journal: Frontiers in Immunology

Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

doi: 10.3389/fimmu.2025.1578372

Figure Lengend Snippet: Dot-plot between anti-CRP antibodies and epitope positivity. The graph displays anti-CRP antibodies versus the number of positive epitopes obtained with microarray-based linear epitope mapping in patients with systemic lupus erythematosus ( n =42). (CRP, C-reactive protein).

Article Snippet: The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany).

Techniques: Microarray

Journal: Frontiers in Immunology

Article Title: Mapping autoantibody targets of full-length C-reactive protein in systemic lupus erythematosus: importance for neutrophil function and classical complement activation

doi: 10.3389/fimmu.2025.1578372

Figure Lengend Snippet: Heatmap representation of autoreactivity against CRP obtained with microarray-based linear epitope mapping of the full CRP monomer. (A, B) Individual signal intensity of IgG autoantibody reactivity against full-length CRP for subjects with SLE ( n =42) and HBD ( n =11). (C–E) Mean signal intensity of IgG autoantibody reactivity against motifs of full-length CRP comparing anti-CRP negative (anti-CRP–; n =16) vs. anti-CRP positive (anti-CRP+; n =26) patients with SLE, patients with ( n =6) and without ( n =36) active disease, and no damage ( n =20) vs. irreversible organ damage (any organ system; n =22). Each column represents one subject (A, B) or the mean value of a group (C–E) . Each row represents a 15 amino acid long sequence covering the full length of the protein with 14 amino acids overlap and 7 amino acid GS repeats elongated before and after the protein sequence. (CRP, C-reactive protein; HBD, healthy blood donors; SDI, Systemic Lupus International Collaborating Clinics/American College of Rheumatology damage index; SLE, systemic lupus erythematosus; SLEDAI-2K, SLE Disease Activity Index 2000).

Article Snippet: The full sequence of the CRP monomer was printed in 15 a.a sequences with 14 a.a overlap using PEPperCHIP Custom Peptide Microarray (PEPperPRINT ® GmbH, Heidelberg, Germany).

Techniques: Microarray, Sequencing, Activity Assay

Journal: Cell Death & Disease

Article Title: Targeting ERBB3 and AKT to overcome adaptive resistance in EML4-ALK-driven non-small cell lung cancer

doi: 10.1038/s41419-024-07272-7

Figure Lengend Snippet: A Schematic model showing the PTK PamChip peptide microarray system and experimental design. B Heat map of up-and down-regulated Tyrosine (PTK assay) bait peptide phosphorylation of lorlatinib (4 h) versus DMSO treatment by peptide chip array ( n = 3 biological replicates) in H3122 and H2228 cell lines. Significant peptides ( p < 0.05) were clustered using hierarchical order and Euclidean/ward algorithm. C , D Volcano plots of the bait peptide phosphorylation from PTK chip arrays in A showing significant up- (Red) and downregulation (blue) of peptide phosphorylation as defined by ANOVA and post-hoc Dunnett’s test versus DMSO treatment ( p < 0.05). Non-significant peptides were highlighted in grey. Downregulated peptides were highlighted in light blue. ERBB family and AKT1 phosphopeptides were highlighted in green and black bold colours, respectively. E Venn diagram was generated to compare the phosphorylated Tyr peptides (up- and downregulated) after a 4 h treatment with lorlatinib in H3122 and H2228 cell lines. Tables of the specific phosphorylated Tyr sites and the associated proteins are shown. The down-regulated proteins were highlighted in cyan colour. The upregulated proteins selected for further analysis were highlighted in bold with green and black colours.

Article Snippet: To identify adaptive response mechanisms, we examined the landscape of tyrosine phosphorylation upon acute LOR treatment in EML4-ALK-positive NSCLC cell lines using PamGene microarray peptide assay, known as protein tyrosine kinase (PTK) (Fig. ).

Techniques: Peptide Microarray, Generated