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pegfp n1 empty vectors  (TaKaRa)


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    Structured Review

    TaKaRa pegfp n1 empty vectors
    Pegfp N1 Empty Vectors, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 3395 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pegfp+empty+vector/pmCherry-N1+Vector/pmc04059699-117-7-10
    Average 96 stars, based on 3395 article reviews
    pegfp n1 empty vectors - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.
    Article Snippet: .. The PCR fragment was cloned between XhoI and BamHI restriction sites of a mCherry-N1 (Clontech) vector. ..

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Clone Assay:

    Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.
    Article Snippet: .. The PCR fragment was cloned between XhoI and BamHI restriction sites of a mCherry-N1 (Clontech) vector. ..

    Article Title: A novel hairless highly immunodeficient mice model optimized for in vivo imaging
    Article Snippet: The human cholangiocarcinoma cell line KKU-213A [ ] and highly pigmented human melanoma cell line Ihara [ , ] were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Wako Pure Chemicals, Osaka, Japan) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 100 U/ml penicillin, 100 μg/ml streptomycin. .. The mCherry-transduced KKU-213A (M213-mCherry) was established by transfection of pmCherry-N1 Vector (Clontech, Mountain View, CA, USA) with the transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and selected in medium containing neomycin (G418; Carbiochem, Darmstadt, Germany), followed by isolation of stable clones by limiting dilution. .. Mouse splenocytes were isolated and stained with DX5-FITC (pan-NK marker), mCD122 (IL-2 Rβ)-PE, mCD19-APC, and mCD3-PE/Cy7 (eBiosciences, San Diego, CA, USA) and analyzed with LSR II (BD Biosciences, San Diego, CA, USA).

    Article Title: A novel hairless highly immunodeficient mice model optimized for in vivo imaging.
    Article Snippet: The human cholangiocarcinoma cell line KKU-213A [10] and highly pigmented human melanoma cell line Ihara [11, 12] were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Wako Pure Chemicals, Osaka, Japan) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 100 U/ml penicillin, 100 μg/ml streptomycin. .. The mCherry-transduced KKU-213A (M213-mCherry) was established by transfection of pmCherry-N1 Vector (Clontech, Mountain View, CA, USA) with the transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and selected in medium containing neomycin (G418; Carbiochem, Darmstadt, Germany), followed by isolation of stable clones by limiting dilution. .. Mouse splenocytes were isolated and stained with DX5FITC (pan-NK marker), mCD122 (IL-2 Rβ)-PE, mCD19APC, and mCD3-PE/Cy7 (eBiosciences, San Diego, CA, USA) and analyzed with LSR II (BD Biosciences, San Diego, CA, USA).

    Plasmid Preparation:

    Article Title: Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.
    Article Snippet: .. The PCR fragment was cloned between XhoI and BamHI restriction sites of a mCherry-N1 (Clontech) vector. ..

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Amplification:

    Article Title: The γ-Tubulin Ring Complex promotes mitotic spindle integrity and acts as a microtubule minus-end cap during mitosis
    Article Snippet: .. The cDNAs of mCherry and puromycin resistance gene were amplified from pmCherry-N1 (632523, Clonetech) and pICE vector (Addgene #46960) ( ) respectively as previously described ( ). .. The gRNAs sequence was synthesized (Azenta) and integrated into pX330 vector (Addgene #42230) using the protocol from the Zhang Lab ( ).

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Transfection:

    Article Title: A novel hairless highly immunodeficient mice model optimized for in vivo imaging
    Article Snippet: The human cholangiocarcinoma cell line KKU-213A [ ] and highly pigmented human melanoma cell line Ihara [ , ] were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Wako Pure Chemicals, Osaka, Japan) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 100 U/ml penicillin, 100 μg/ml streptomycin. .. The mCherry-transduced KKU-213A (M213-mCherry) was established by transfection of pmCherry-N1 Vector (Clontech, Mountain View, CA, USA) with the transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and selected in medium containing neomycin (G418; Carbiochem, Darmstadt, Germany), followed by isolation of stable clones by limiting dilution. .. Mouse splenocytes were isolated and stained with DX5-FITC (pan-NK marker), mCD122 (IL-2 Rβ)-PE, mCD19-APC, and mCD3-PE/Cy7 (eBiosciences, San Diego, CA, USA) and analyzed with LSR II (BD Biosciences, San Diego, CA, USA).

    Article Title: A novel hairless highly immunodeficient mice model optimized for in vivo imaging.
    Article Snippet: The human cholangiocarcinoma cell line KKU-213A [10] and highly pigmented human melanoma cell line Ihara [11, 12] were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Wako Pure Chemicals, Osaka, Japan) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 100 U/ml penicillin, 100 μg/ml streptomycin. .. The mCherry-transduced KKU-213A (M213-mCherry) was established by transfection of pmCherry-N1 Vector (Clontech, Mountain View, CA, USA) with the transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and selected in medium containing neomycin (G418; Carbiochem, Darmstadt, Germany), followed by isolation of stable clones by limiting dilution. .. Mouse splenocytes were isolated and stained with DX5FITC (pan-NK marker), mCD122 (IL-2 Rβ)-PE, mCD19APC, and mCD3-PE/Cy7 (eBiosciences, San Diego, CA, USA) and analyzed with LSR II (BD Biosciences, San Diego, CA, USA).

    Isolation:

    Article Title: A novel hairless highly immunodeficient mice model optimized for in vivo imaging
    Article Snippet: The human cholangiocarcinoma cell line KKU-213A [ ] and highly pigmented human melanoma cell line Ihara [ , ] were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Wako Pure Chemicals, Osaka, Japan) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 100 U/ml penicillin, 100 μg/ml streptomycin. .. The mCherry-transduced KKU-213A (M213-mCherry) was established by transfection of pmCherry-N1 Vector (Clontech, Mountain View, CA, USA) with the transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and selected in medium containing neomycin (G418; Carbiochem, Darmstadt, Germany), followed by isolation of stable clones by limiting dilution. .. Mouse splenocytes were isolated and stained with DX5-FITC (pan-NK marker), mCD122 (IL-2 Rβ)-PE, mCD19-APC, and mCD3-PE/Cy7 (eBiosciences, San Diego, CA, USA) and analyzed with LSR II (BD Biosciences, San Diego, CA, USA).

    Article Title: A novel hairless highly immunodeficient mice model optimized for in vivo imaging.
    Article Snippet: The human cholangiocarcinoma cell line KKU-213A [10] and highly pigmented human melanoma cell line Ihara [11, 12] were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Wako Pure Chemicals, Osaka, Japan) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS; JRH Bioscience, Lenexa, KS, USA), 100 U/ml penicillin, 100 μg/ml streptomycin. .. The mCherry-transduced KKU-213A (M213-mCherry) was established by transfection of pmCherry-N1 Vector (Clontech, Mountain View, CA, USA) with the transfection reagent Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) according to the manufacturer’s instructions, and selected in medium containing neomycin (G418; Carbiochem, Darmstadt, Germany), followed by isolation of stable clones by limiting dilution. .. Mouse splenocytes were isolated and stained with DX5FITC (pan-NK marker), mCD122 (IL-2 Rβ)-PE, mCD19APC, and mCD3-PE/Cy7 (eBiosciences, San Diego, CA, USA) and analyzed with LSR II (BD Biosciences, San Diego, CA, USA).

    Sequencing:

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    Cloning:

    Article Title: Role of calcineurin B homologous protein 3 in C2C12 myogenic differentiation
    Article Snippet: Transient overexpression of CHP3 in C2C12 cells Total RNA was extracted from C2C12 cells using the RNeasy Mini Kit (Qiagen, Valencia, CA, USA) and reversetranscribed into cDNA with ReverTra Ace -α- (Toyobo, Osaka, Japan). .. The coding sequence of mouse CHP3 was amplified by PCR and subcloned in-frame upstream of mCherry in the pmCherry-N1 vector (Takara Bio, Shiga, Japan) using the In-Fusion HD Cloning Kit (Takara Bio). ..

    other:

    Article Title: Membranes arrest the coarsening of mitochondrial condensates in human cells.
    Article Snippet: The manufacturer’s protocol from Thermo Scientific RevertAid Reverse Transcriptase (EP0441) was followed using Oligo(dT)18 as primer for the first strand cDNA synthesis, RNase inhibitor (Invitrogen), and dNTP mixture (TaKaRa).



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