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pe buffer  (Qiagen)


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    Structured Review

    Qiagen pe buffer
    Pe Buffer, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 576 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pe+buffer/Buffer+PE/pmc13133277-252-13-15
    Average 97 stars, based on 576 article reviews
    pe buffer - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    other:

    Article Title: Papuan Admixture Predated the Settlement of Palau
    Article Snippet: Buffer PE concentrate , QIAGEN , 19065.

    Article Title: Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination.
    Article Snippet: Carefully open the glass plates and stain the gel in 100 mL of deionized water with 10 μL of SYBR Gold (Invitrogen) for 10 min. 35.

    Article Title: Protocol for integrative analysis of transcription factor-nucleosome interactions using SeEN-seq and cryo-EM structure determination
    Article Snippet: Add 750 μL of PE buffer (Qiagen) and centrifuge at 13,000 rpm (15,871 x g) for 1 min. 48.

    Isolation:

    Article Title: BRD4 recruitment desilences transcription without erasure or depletion of repressive chromatin
    Article Snippet: .. DNA was isolated using Qiagen DNA cleanup buffers PB (19066) and PE (19065). ..

    Polymerase Chain Reaction:

    Article Title: DMS-MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity.
    Article Snippet: .. The PCR products were gel purified using Qiagen QG (cat#19063) and PE (cat#19065) buffer. .. The in vitro transcription reaction containing 250 ng templates, 1 μL of 100 m m ATG, 1 μL of 100 m CTP, 1 μL of 100 m GTP, 0.4 μL of 100 m UTP, 0.8 μL of 50 m biotin-16-UTP (Lucigen), 1.5 μL T7 RNA polymerase (lab purified), and 1 μL Superase-In RNase inhibitor (Invitrogen cat#AM2696) in a total of 20 μL was incubated at 37◦C 13 of 18 tive C om m ons L icense o a w R o 1 1 m R e 4 T S p f R 1 R b t m 5 T d w k r 4 D 5 d S I l 4 T M 4 R t r T f q o 9 q t 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.75614 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [12/05/2026].

    Purification:

    Article Title: DMS-MaPseq and DREEM Analyses Implicate the Critical Role of RNA Structural Dynamics in Turnip Yellow Mosaic Virus Pathogenicity.
    Article Snippet: .. The PCR products were gel purified using Qiagen QG (cat#19063) and PE (cat#19065) buffer. .. The in vitro transcription reaction containing 250 ng templates, 1 μL of 100 m m ATG, 1 μL of 100 m CTP, 1 μL of 100 m GTP, 0.4 μL of 100 m UTP, 0.8 μL of 50 m biotin-16-UTP (Lucigen), 1.5 μL T7 RNA polymerase (lab purified), and 1 μL Superase-In RNase inhibitor (Invitrogen cat#AM2696) in a total of 20 μL was incubated at 37◦C 13 of 18 tive C om m ons L icense o a w R o 1 1 m R e 4 T S p f R 1 R b t m 5 T d w k r 4 D 5 d S I l 4 T M 4 R t r T f q o 9 q t 1 nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.75614 by loki revolution - U niversity Federal D e Sao Paulo , W iley O nline L ibrary on [12/05/2026].



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    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: BCoV infection induces the expression of PANoptosis-related genes in MDBK cells. A Western blot analysis of RIPK3, RIPK1, p-MLKL, and ZBP1 protein levels. B , C Relative mRNA levels of ZBP1 ( B ) and RIPK3 ( C ) determined by qPCR. D , E Protein ( D ) and mRNA ( E ) expression of CASP8. F Flow cytometric analysis of apoptosis via Annexin V-PE/7-AAD staining in control and BCoV-infected cells. G , H Protein ( G ) and mRNA ( H ) expression of GSDMD, CASP1, and ASC. I Relative mRNA expression of IL-1β . J , K Protein ( J ) and mRNA ( K ) expression of Neu1. L Cellular sialic acid levels, which decreased in a dose-dependent manner upon BCoV infection. Quantitative densitometry of the western blot results (normalized to β-actin/GAPDH) is shown in Additional file . The data are presented as the means ± SDs ( n = 3). Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Expressing, Western Blot, Staining, Control

    The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: Veterinary Research

    Article Title: Neu1 inhibition restrains BCoV replication and modulates ZBP1-dependent PANoptosis

    doi: 10.1186/s13567-026-01729-7

    Figure Lengend Snippet: The interaction between Neu1 and ZBP1 regulates apoptosis and sialic acid levels in BCoV-infected MDBK cells. A , B Schematic representation and validation of stable MDBK Neu1kd and MDBK ZBP1kd cell lines. C Apoptosis analysis of WT, MDBK Neu1kd , and MDBK ZBP1kd cells following BCoV infection via Annexin V-PE/7-AAD flow cytometry. D , E Viral titers determined via the Reed–Muench method ( D ) and relative BCoV mRNA expression levels ( E ). F Immunofluorescence localization of Neu1 (red) and ZBP1 (green) in WT, MDBK Neu1kd , and MDBK ZBP1kd cells. Nuclei were stained with DAPI (blue), and merged signals (yellow) indicate colocalization. G Cellular sialic acid levels significantly decreased in both MDBK Neu1kd and MDBK ZBP1kd cells. H Immunofluorescence analysis further demonstrated that Neu1 or ZBP1 knockdown markedly reduced the fluorescence signal intensity during BCoV infection. The data are presented as the means ± SDs ( n = 3). The quantification of fluorescence intensity is shown in Additional file . Statistical significance: * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: The pellets were washed three times with 1× PBS and subsequently resuspended in 1× Annexin V-PE binding buffer (0.1 M HEPES, 1.4 M NaCl, and 25 mM CaCl 2 solution; Servicebio, Wuhan) at the appropriate cell density.

    Techniques: Infection, Biomarker Discovery, Flow Cytometry, Endpoint Dilution Assay, Expressing, Immunofluorescence, Staining, Knockdown, Fluorescence