pcatbasic vector (Promega)
90
Structured Review
Promega
pcatbasic vector
Pcatbasic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcatbasic+vector/pcatbasic+vector/pm09840920-157-42-45
Average 90 stars, based on 1 article reviews
Pcatbasic Vector, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pcatbasic+vector/pcatbasic+vector/pm09840920-157-42-45
Average 90 stars, based on 1 article reviews
pcatbasic vector - by Bioz Stars,
2026-09
90/100 stars
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Plasmid Preparation:Article Title: Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1 Article Snippet: .. The resulting α6 promoter-bearing DNA fragment was then ligated upstream the CAT reporter gene from the Article Title: WS5, a direct target of oncogenic transcription factor Myc, is related to human melanoma glycoprotein genes and has oncogenic potential. Article Snippet: .. For construction of the CAT reporter plasmids pCAT-WS5 and pCAT-WS5-L, DNA fragments from the WS5 promoter region with sizes of 276 and 1298 bp corresponding to nucleotides 1124–1399 and 102–1399 in the 6017-bp genomic WS5 sequence (accession no. AY496440) were amplified by PCR and inserted into the HindIII/XbaI sites of the Article Title: Laminin reduces expression of the human alpha6 integrin subunit gene by altering the level of the transcription factors Sp1 and Sp3. Article Snippet: METHODS.. Recombinant plasmids bearing the CAT reporter gene, fused to various segments from the 6 promoter, were transfected into rabbit corneal epithelial cells (RCECs) grown on untreated or on LM-coated culture plates or into Sp1-deficient Drosophila Schneider cells.. Expression and DNA binding of Sp1/Sp3 was monitored with the use of Western blot and electrophoretic mobility shift assays (EMSAs), respectively. Article Title: Antisense experiments reveal molecular details on mechanisms of ICER suppressing cAMP-inducible genes in rat pinealocytes. Article Snippet: In the rat pineal gland neuronal signals determine the rhythmic synthesis of the hormone melatonin.. Norepinephrine (NE) is the principal neurotransmitter that drives hormone synthesis by activating the cAMP signaling pathway.. This activation depends on transcriptional and posttranscriptional regulatory mechanisms. Article Title: Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1 Article Snippet: .. Reporter chloramphenicol acetyltransferase (CAT) construct pSMpCAT1 contains 1148 bp of the 5«-flanking region and the first 28 bp of exon 1 of the rat HMGCS2 gene in the Article Title: Proximal promoter-independent activation of the far-upstream FGF-inducible response element of syndecan-1 gene. Article Snippet: s s p o w t i b i v h Far upstream enhancers are predicted to act by ooping and activating general transcription factors n core promoters and to require proximal promoter equences for appropriate gene activation in time and pace.. We have previously described an FGF-inducible esponse element (FiRE) located far upstream on the yndecan-1 gene.. The FiRE is activated specifically by embers of the fibroblast growth factor (FGF) family n NIH3T3 cells. Article Title: Structural diversity despite strong evolutionary conservation in the 5'-untranslated region of the P-type dystrophin transcript. Article Snippet: *Department of Anatomy and Developmental Biology, Division of Basic Medical Sciences, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom; Department of Pharmacology, Tsukuba Research Laboratory, GlaxoWellcome, 43 Wadai, Tsukuba 300-4247, Japan; and Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QJ, United Kingdom De-Phosphorylation Assay:Article Title: Regulation of poly(ADP-ribose) polymerase-1 (PARP-1) gene expression through the post-translational modification of Sp1: a nuclear target protein of PARP-1 Article Snippet: .. The resulting α6 promoter-bearing DNA fragment was then ligated upstream the CAT reporter gene from the Article Title: Laminin reduces expression of the human alpha6 integrin subunit gene by altering the level of the transcription factors Sp1 and Sp3. Article Snippet: METHODS.. Recombinant plasmids bearing the CAT reporter gene, fused to various segments from the 6 promoter, were transfected into rabbit corneal epithelial cells (RCECs) grown on untreated or on LM-coated culture plates or into Sp1-deficient Drosophila Schneider cells.. Expression and DNA binding of Sp1/Sp3 was monitored with the use of Western blot and electrophoretic mobility shift assays (EMSAs), respectively. Sequencing:Article Title: WS5, a direct target of oncogenic transcription factor Myc, is related to human melanoma glycoprotein genes and has oncogenic potential. Article Snippet: .. For construction of the CAT reporter plasmids pCAT-WS5 and pCAT-WS5-L, DNA fragments from the WS5 promoter region with sizes of 276 and 1298 bp corresponding to nucleotides 1124–1399 and 102–1399 in the 6017-bp genomic WS5 sequence (accession no. AY496440) were amplified by PCR and inserted into the HindIII/XbaI sites of the Amplification:Article Title: WS5, a direct target of oncogenic transcription factor Myc, is related to human melanoma glycoprotein genes and has oncogenic potential. Article Snippet: .. For construction of the CAT reporter plasmids pCAT-WS5 and pCAT-WS5-L, DNA fragments from the WS5 promoter region with sizes of 276 and 1298 bp corresponding to nucleotides 1124–1399 and 102–1399 in the 6017-bp genomic WS5 sequence (accession no. AY496440) were amplified by PCR and inserted into the HindIII/XbaI sites of the Polymerase Chain Reaction:Article Title: WS5, a direct target of oncogenic transcription factor Myc, is related to human melanoma glycoprotein genes and has oncogenic potential. Article Snippet: .. For construction of the CAT reporter plasmids pCAT-WS5 and pCAT-WS5-L, DNA fragments from the WS5 promoter region with sizes of 276 and 1298 bp corresponding to nucleotides 1124–1399 and 102–1399 in the 6017-bp genomic WS5 sequence (accession no. AY496440) were amplified by PCR and inserted into the HindIII/XbaI sites of the Article Title: Regulation of WNK4 gene transcription in the kidneys Article Snippet: .. The PCR product was cloned into a pDM18-T vector, and subcloned into a unique Hindβ/XbaI site of a Clone Assay:Article Title: Regulation of WNK4 gene transcription in the kidneys Article Snippet: .. The PCR product was cloned into a pDM18-T vector, and subcloned into a unique Hindβ/XbaI site of a Article Title: Structural diversity despite strong evolutionary conservation in the 5'-untranslated region of the P-type dystrophin transcript. Article Snippet: *Department of Anatomy and Developmental Biology, Division of Basic Medical Sciences, Royal Free and University College Medical School, Rowland Hill Street, London NW3 2PF, United Kingdom; Department of Pharmacology, Tsukuba Research Laboratory, GlaxoWellcome, 43 Wadai, Tsukuba 300-4247, Japan; and Department of Pharmacology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QJ, United Kingdom Construct:Article Title: Regulation of WNK4 gene transcription in the kidneys Article Snippet: .. The PCR product was cloned into a pDM18-T vector, and subcloned into a unique Hindβ/XbaI site of a Article Title: Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1 Article Snippet: .. Reporter chloramphenicol acetyltransferase (CAT) construct pSMpCAT1 contains 1148 bp of the 5«-flanking region and the first 28 bp of exon 1 of the rat HMGCS2 gene in the |