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sox2 expression vectors  (Addgene inc)


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    Structured Review

    Addgene inc sox2 expression vectors
    Sox2 Expression Vectors, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcaf+expression+vector/408+pSG5L+HA+E2F1+(Plasmid+%2310736)/pmc03427024-86-3-9
    Average 93 stars, based on 22 article reviews
    sox2 expression vectors - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    other:

    Article Title: RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling
    Article Snippet: The recombinant human WNT4 protein (RnD Systems; Cat. No. 6076-Wn; >60% purity), recombinant human WNT8A (Genemed PlexBio; Cat No. 90007-02; >95% purity).

    Article Title: Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma.
    Article Snippet: Article Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma

    Sequencing:

    Article Title: An E2F1/MiR-17-92 Negative Feedback Loop mediates proliferation of Mouse Palatal Mesenchymal Cells.
    Article Snippet: .. Vectors of shE2F1 (Origene TG509487), shSCR (vectors against a scrambled sequence, negative control (Origene TR30013), and E2F1 expression vectors (Addgene plasmid 10736) were transfected into PMCs with TurboFectin 8.0 (Origene, Rockville, MD, USA) following the manufacturer’s protocol. ..

    Negative Control:

    Article Title: An E2F1/MiR-17-92 Negative Feedback Loop mediates proliferation of Mouse Palatal Mesenchymal Cells.
    Article Snippet: .. Vectors of shE2F1 (Origene TG509487), shSCR (vectors against a scrambled sequence, negative control (Origene TR30013), and E2F1 expression vectors (Addgene plasmid 10736) were transfected into PMCs with TurboFectin 8.0 (Origene, Rockville, MD, USA) following the manufacturer’s protocol. ..

    Expressing:

    Article Title: An E2F1/MiR-17-92 Negative Feedback Loop mediates proliferation of Mouse Palatal Mesenchymal Cells.
    Article Snippet: .. Vectors of shE2F1 (Origene TG509487), shSCR (vectors against a scrambled sequence, negative control (Origene TR30013), and E2F1 expression vectors (Addgene plasmid 10736) were transfected into PMCs with TurboFectin 8.0 (Origene, Rockville, MD, USA) following the manufacturer’s protocol. ..

    Article Title: E2F1 promotes cancer cell sensitivity to cisplatin by regulating the cellular DNA damage response through miR-26b in esophageal squamous cell carcinoma
    Article Snippet: .. The E2F1 expression plasmid 408 pSG5L-HA-E2F1 was obtained from Addgene (#10736) . .. Luciferase assays were performed using the pGL3-miR-26b-promoter vector containing the 26-bp DNA sequence of the miR-26b gene promoter region.

    Article Title: E2F1 Reduces Sorafenib's Sensitivity of Esophageal Carcinoma Cells via Modulating the miR-29c-3p/COL11A1 Signaling Axis.
    Article Snippet: The dual-luciferase activity was examined on Promega’s Digital-Luciferase Reporter Assay System (Promega, USA). .. Referring to the method for vector construction mentioned by Kun Zhang et al. [6], E2F1 expression plasmid 408 pSG5LHA-E2F1 was produced by Addgene (#10736). ..

    Plasmid Preparation:

    Article Title: An E2F1/MiR-17-92 Negative Feedback Loop mediates proliferation of Mouse Palatal Mesenchymal Cells.
    Article Snippet: .. Vectors of shE2F1 (Origene TG509487), shSCR (vectors against a scrambled sequence, negative control (Origene TR30013), and E2F1 expression vectors (Addgene plasmid 10736) were transfected into PMCs with TurboFectin 8.0 (Origene, Rockville, MD, USA) following the manufacturer’s protocol. ..

    Article Title: Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.
    Article Snippet: .. Developmental Cell 56, 2103–2120.e1–e9, July 26, 2021 e5 Plasmid construction and cell transfection Plasmids pLK65(#37257), pRK5-HA-GST-Rheb1 (#14951), pSG5L-HA-E2F1 (#10736) and pPAmCherry1-N1 (#31928) were purchased from Addgene (Cambridge, MA, USA). .. The Vangl2 sequence from pLK65 was cloned into pRK5-HA-GST, between the SalI and NotI restriction sites, after excision of the Rheb1 sequence from pRK5-HA-GST-Rheb1. pSG5L-vangl2 was constructed from pSG5L-HA-E2F1 by replacing HA and E2F1 with the Vangl2 sequence using HindIII and EcoRI restriction sites.

    Article Title: E2F1 promotes cancer cell sensitivity to cisplatin by regulating the cellular DNA damage response through miR-26b in esophageal squamous cell carcinoma
    Article Snippet: .. The E2F1 expression plasmid 408 pSG5L-HA-E2F1 was obtained from Addgene (#10736) . .. Luciferase assays were performed using the pGL3-miR-26b-promoter vector containing the 26-bp DNA sequence of the miR-26b gene promoter region.

    Article Title: E2F1 Reduces Sorafenib's Sensitivity of Esophageal Carcinoma Cells via Modulating the miR-29c-3p/COL11A1 Signaling Axis.
    Article Snippet: The dual-luciferase activity was examined on Promega’s Digital-Luciferase Reporter Assay System (Promega, USA). .. Referring to the method for vector construction mentioned by Kun Zhang et al. [6], E2F1 expression plasmid 408 pSG5LHA-E2F1 was produced by Addgene (#10736). ..

    Article Title: RBL2-E2F-GCN5 guide cell fate decisions during tissue specification by regulating cell-cycle-dependent fluctuations of non-cell-autonomous signaling.
    Article Snippet: 4xCSL-luciferase was a gift fromRaphael Kopan (Addgene plasmid # 41726). .. 408 pSG5L HA E2F1 was a gift from William Sellers (Addgene plasmid # 10736; http://n2t.net/addgene:10736; RRID:Addgene_10736). .. 409 pSG5L HA RB (379–928) was a gift from William Sellers (Addgene plasmid # 10734; http://n2t.net/addgene:10734; RRID:Addgene_10734).

    Transfection:

    Article Title: An E2F1/MiR-17-92 Negative Feedback Loop mediates proliferation of Mouse Palatal Mesenchymal Cells.
    Article Snippet: .. Vectors of shE2F1 (Origene TG509487), shSCR (vectors against a scrambled sequence, negative control (Origene TR30013), and E2F1 expression vectors (Addgene plasmid 10736) were transfected into PMCs with TurboFectin 8.0 (Origene, Rockville, MD, USA) following the manufacturer’s protocol. ..

    Article Title: Vangl2 limits chaperone-mediated autophagy to balance osteogenic differentiation in mesenchymal stem cells.
    Article Snippet: .. Developmental Cell 56, 2103–2120.e1–e9, July 26, 2021 e5 Plasmid construction and cell transfection Plasmids pLK65(#37257), pRK5-HA-GST-Rheb1 (#14951), pSG5L-HA-E2F1 (#10736) and pPAmCherry1-N1 (#31928) were purchased from Addgene (Cambridge, MA, USA). .. The Vangl2 sequence from pLK65 was cloned into pRK5-HA-GST, between the SalI and NotI restriction sites, after excision of the Rheb1 sequence from pRK5-HA-GST-Rheb1. pSG5L-vangl2 was constructed from pSG5L-HA-E2F1 by replacing HA and E2F1 with the Vangl2 sequence using HindIII and EcoRI restriction sites.

    Produced:

    Article Title: E2F1 Reduces Sorafenib's Sensitivity of Esophageal Carcinoma Cells via Modulating the miR-29c-3p/COL11A1 Signaling Axis.
    Article Snippet: The dual-luciferase activity was examined on Promega’s Digital-Luciferase Reporter Assay System (Promega, USA). .. Referring to the method for vector construction mentioned by Kun Zhang et al. [6], E2F1 expression plasmid 408 pSG5LHA-E2F1 was produced by Addgene (#10736). ..



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