Review



chloramphenicol acetyltransferase reporter vector (pcat control  (Promega)

 
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    Structured Review

    Promega chloramphenicol acetyltransferase reporter vector (pcat control
    Chloramphenicol Acetyltransferase Reporter Vector (Pcat Control, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcaf+expression+vector/chloramphenicol+acetyltransferase/pmc00103898-180-3-9
    Average 90 stars, based on 1 article reviews
    chloramphenicol acetyltransferase reporter vector (pcat control - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: O(6) -methylguanine DNA methyltransferase repairs platinum-DNA adducts following cisplatin treatment and predicts prognoses of nasopharyngeal carcinoma.
    Article Snippet: Host cell reactivation assay The chloramphenicol acetyltransferase (CAT) gene (Promega) was used as the CDDP-damage reporter DNA, and a pSV b-galactosidase (b-gal) control vector served as an internal control for transfection efficiency as described previously.20,26 CAT-containing plasmid DNA, treated with or without CDDP, was incubated with 3 mM NaCl1 mM sodium phosphate (pH 7.4) buffer at 37 C for 1 hr followed by ethanol precipitation.

    Article Title: Neuropathology and Cellular Pathogenesis of Spinocerebellar Ataxia Type 12
    Article Snippet: Luciferase constructs were then generated from the chloramphenicol acetyltransferase (CAT) constructs by removing the 1.7-kilobase (kb) promoter region and cloning into pGL3-Basic (luciferase vector; Promega) ( Supporting Fig. 1 ).

    Article Title: Rolling Circle Translation of Circular RNA in Living Human Cells
    Article Snippet: The EMCV-IRES-containing bicistronic vector, pβGal-IRES-CAT, was constructed by inserting the β-galactosidase (β-gal) gene from pCMV-LacZ Vector (Clontech, Mountain View, CA, USA) and the chloramphenicol acetyltransferase (CAT) gene from pCAT3-Basic Vector (Promega) into multi-cloning sites A and B of pIRES (Clontech), respectively.

    Amplification:

    Article Title: Mutation of the NADH Oxidase Gene ( nox ) Reveals an Overlap of the Oxygen- and Acid-Mediated Stress Responses in Streptococcus mutans
    Article Snippet: .. A chloramphenicol acetyltransferase gene (Promega, Madison, WI) was amplified using NewCATFwd and NewCATRev, followed by a BamHI digestion. ..

    Activity Assay:

    Article Title: Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines
    Article Snippet: .. Chloramphenicol acetyltransferase (CAT) activity from pCAT (Promega) in lysates was measured following the manufacturer’s protocol. ..

    Article Title: Calcineurin Regulates Nuclear Factor I Dephosphorylation and Activity in Malignant Glioma Cell Lines
    Article Snippet: .. Chloramphenicol acetyltransferase (CAT) activity from pCAT (Promega) in lysates was measured following the manufacturer's protocol. .. Chloramphenicol acetyltransferase (CAT) activity from pCAT (Promega) in lysates was measured following the manufacturer's protocol.

    Construct:

    Article Title: HIV-1 Nef-mediated T-cell activation and chemotaxis are decoupled using a HIV-1/SIVpbj1.9. chimeric nef variant.
    Article Snippet: HIV-1 Nef is known to activate CD4?. T cells but inhibit their migration toward SDF-1a.. However, it is not clear how this protein orchestrates these two seemingly concomitant events.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: HIV-1 Nef-mediated T-cell activation and chemotaxis are decoupled using a HIV-1/SIVpbj1.9. chimeric nef variant.
    Article Snippet: HIV-1 Nef is known to activate CD4?. T cells but inhibit their migration toward SDF-1a.. However, it is not clear how this protein orchestrates these two seemingly concomitant events.

    Isolation:

    Article Title: HIV-1 Nef-mediated T-cell activation and chemotaxis are decoupled using a HIV-1/SIVpbj1.9. chimeric nef variant.
    Article Snippet: HIV-1 Nef is known to activate CD4?. T cells but inhibit their migration toward SDF-1a.. However, it is not clear how this protein orchestrates these two seemingly concomitant events.

    Polymerase Chain Reaction:

    Article Title: HIV-1 Nef-mediated T-cell activation and chemotaxis are decoupled using a HIV-1/SIVpbj1.9. chimeric nef variant.
    Article Snippet: HIV-1 Nef is known to activate CD4?. T cells but inhibit their migration toward SDF-1a.. However, it is not clear how this protein orchestrates these two seemingly concomitant events.

    Cloning:

    Article Title: HIV-1 Nef-mediated T-cell activation and chemotaxis are decoupled using a HIV-1/SIVpbj1.9. chimeric nef variant.
    Article Snippet: HIV-1 Nef is known to activate CD4?. T cells but inhibit their migration toward SDF-1a.. However, it is not clear how this protein orchestrates these two seemingly concomitant events.



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