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pbluescript sk vector  (Addgene inc)


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    Structured Review

    Addgene inc pbluescript sk vector
    Pbluescript Sk Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vector/pBluescript+SK+%2B%2F-+mTRF1+(Plasmid+%2312303)/pmc12595211-76-27-35
    Average 93 stars, based on 39 article reviews
    pbluescript sk vector - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort.
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II- ) vector (Addgene). ..

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID : Insights From Russian Validation Cohort
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa‐deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II‐) vector (Addgene). ..

    Article Title:
    Article Snippet: .. A 2265-bp PCR fragment encompassing the locus was amplified by using the following oligonucleotides (SPfim3UP2: GAGCTCTTTACCGCGGCCGCCAGTTGTTCATCAATG (SEQ ID NO: 1) and ASPfim3LO2: GGATCCATCATCGAGACCGACTGG (SEQ ID NO: 2)) and cloned into the SacI and BamHI restriction sites of a pBluescript II SK+ plasmid (Addgene). ..

    Amplification:

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort.
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II- ) vector (Addgene). ..

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID : Insights From Russian Validation Cohort
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa‐deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II‐) vector (Addgene). ..

    Article Title:
    Article Snippet: .. A 2265-bp PCR fragment encompassing the locus was amplified by using the following oligonucleotides (SPfim3UP2: GAGCTCTTTACCGCGGCCGCCAGTTGTTCATCAATG (SEQ ID NO: 1) and ASPfim3LO2: GGATCCATCATCGAGACCGACTGG (SEQ ID NO: 2)) and cloned into the SacI and BamHI restriction sites of a pBluescript II SK+ plasmid (Addgene). ..

    Clone Assay:

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort.
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II- ) vector (Addgene). ..

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID : Insights From Russian Validation Cohort
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa‐deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II‐) vector (Addgene). ..

    Article Title:
    Article Snippet: .. A 2265-bp PCR fragment encompassing the locus was amplified by using the following oligonucleotides (SPfim3UP2: GAGCTCTTTACCGCGGCCGCCAGTTGTTCATCAATG (SEQ ID NO: 1) and ASPfim3LO2: GGATCCATCATCGAGACCGACTGG (SEQ ID NO: 2)) and cloned into the SacI and BamHI restriction sites of a pBluescript II SK+ plasmid (Addgene). ..

    Plasmid Preparation:

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID: Insights From Russian Validation Cohort.
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa- deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II- ) vector (Addgene). ..

    Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass
    Article Snippet: .. A phagemid based on pBluescript SK- (pDHJS4 AS- ;) was obtained from Addgene (#78243) and transformed into competent E. coli DH5α cells carrying a helper plasmid (HP4_M13 ) (Addgene #120340). ..

    Article Title: Harmonizing TREC Thresholds in Newborn Screening for SCID : Insights From Russian Validation Cohort
    Article Snippet: .. Human albumin, δREC–ψJα T cell receptor, and kappa‐deleting joint of KREC were PCR amplified from DNA obtained from the peripheral blood of a healthy donor and separately cloned into the pBlueScript SK(II‐) vector (Addgene). ..

    Article Title:
    Article Snippet: .. A 2265-bp PCR fragment encompassing the locus was amplified by using the following oligonucleotides (SPfim3UP2: GAGCTCTTTACCGCGGCCGCCAGTTGTTCATCAATG (SEQ ID NO: 1) and ASPfim3LO2: GGATCCATCATCGAGACCGACTGG (SEQ ID NO: 2)) and cloned into the SacI and BamHI restriction sites of a pBluescript II SK+ plasmid (Addgene). ..

    Article Title: A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species.
    Article Snippet: Plasmids pcDNA 3.1/V5-His-TOPO and pcDNA 3.1/V5-His-TOPO/LacZ were part of the pcDNA 3.1/V5-His-TOPO Expression Kit (ThermoFisher Scientific, Waltham, MA, USA). .. Plasmid pBluescript SK (+) containing a large and convenient for subcloning purposes linker region was obtained from Addgene (Watertown, MA, USA). .. Plasmid pBV-Luc containing the luciferase reporter gene was also obtained from Addgene.

    Article Title: A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species
    Article Snippet: Plasmids pcDNA 3.1/V5-His-TOPO and pcDNA 3.1/V5-His-TOPO/LacZ were part of the pcDNA 3.1/V5-His-TOPO Expression Kit (ThermoFisher Scientific, Waltham, MA, USA). .. Plasmid pBluescript SK (+) containing a large and convenient for subcloning purposes linker region was obtained from Addgene (Watertown, MA, USA). .. Plasmid pBV-Luc containing the luciferase reporter gene was also obtained from Addgene.

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. The ERα cDNA of the human species was bought from Addgene (Cambridge, MA, USA) and was further cultivated by cloning into the EcoRI restriction site of the pBluescript SK vector, which was also bought from Addgene. ..

    Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass.
    Article Snippet: .. A phagemid based on pBluescript SK- (pDHJS4 AS-54;) was obtained from Addgene (#78243) and transformed into competent E. coli DH5α cells carrying a helper plasmid (HP4_M1355) (Addgene #120340). ..

    Transformation Assay:

    Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass
    Article Snippet: .. A phagemid based on pBluescript SK- (pDHJS4 AS- ;) was obtained from Addgene (#78243) and transformed into competent E. coli DH5α cells carrying a helper plasmid (HP4_M13 ) (Addgene #120340). ..

    Article Title: The SMC Hinge is a Selective Gate for Obstacle Bypass.
    Article Snippet: .. A phagemid based on pBluescript SK- (pDHJS4 AS-54;) was obtained from Addgene (#78243) and transformed into competent E. coli DH5α cells carrying a helper plasmid (HP4_M1355) (Addgene #120340). ..

    Subcloning:

    Article Title: A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species.
    Article Snippet: Plasmids pcDNA 3.1/V5-His-TOPO and pcDNA 3.1/V5-His-TOPO/LacZ were part of the pcDNA 3.1/V5-His-TOPO Expression Kit (ThermoFisher Scientific, Waltham, MA, USA). .. Plasmid pBluescript SK (+) containing a large and convenient for subcloning purposes linker region was obtained from Addgene (Watertown, MA, USA). .. Plasmid pBV-Luc containing the luciferase reporter gene was also obtained from Addgene.

    Article Title: A Repetitive Acipenser gueldenstaedtii Genomic Region Aligning with the Acipenser baerii IGLV Gene Cluster Suggests a Role as a Transcription Termination Element Across Several Sturgeon Species
    Article Snippet: Plasmids pcDNA 3.1/V5-His-TOPO and pcDNA 3.1/V5-His-TOPO/LacZ were part of the pcDNA 3.1/V5-His-TOPO Expression Kit (ThermoFisher Scientific, Waltham, MA, USA). .. Plasmid pBluescript SK (+) containing a large and convenient for subcloning purposes linker region was obtained from Addgene (Watertown, MA, USA). .. Plasmid pBV-Luc containing the luciferase reporter gene was also obtained from Addgene.

    Cloning:

    Article Title: Quercetin suppresses endometrial cancer stem cells via ERα-mediated inhibition of STAT3 signaling
    Article Snippet: .. The ERα cDNA of the human species was bought from Addgene (Cambridge, MA, USA) and was further cultivated by cloning into the EcoRI restriction site of the pBluescript SK vector, which was also bought from Addgene. ..



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    Image Search Results


    Journal: bioRxiv

    Article Title: CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

    doi: 10.1101/2025.11.18.688292

    Figure Lengend Snippet:

    Article Snippet: Briefly, Firefly Luciferase plasmid and the repeat containing pBluescript vectors were digested with XbaI and NotI restriction enzymes (NEB), while pGint plasmid and pBluescript vectors were digested with NotI and BamHI restriction enzymes (NEB) at 37°C for 1 hour.

    Techniques: Luciferase