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pbluescript ks lentiviral ires gfp vector lenticrisprv2 puro  (Addgene inc)


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    Addgene inc pbluescript ks lentiviral ires gfp vector lenticrisprv2 puro
    Pbluescript Ks Lentiviral Ires Gfp Vector Lenticrisprv2 Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 98/100, based on 527 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbluescript+vector/lentiCRISPRv2+puro+(Plasmid+%2398290)/pmc12549880-5-0-10
    Average 98 stars, based on 527 article reviews
    pbluescript ks lentiviral ires gfp vector lenticrisprv2 puro - by Bioz Stars, 2026-11
    98/100 stars

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    Generated:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Article Title: Splicing-guaranteed artificial introns enable efficient homology-directed repair at internal exons and streamlined cell engineering
    Article Snippet: .. DSBs were generated by CRISPR–Cas9 technology with LentiCRISPR-V2 (Addgene, #98290) carrying the indicated intron-targeting sgRNAs (RELA: 5’-UCA CUG AGC UCC CGG UCG GA-3’, EGFR: 5’-GCU GGC CAA ACU GCU GGG UG-3’, PIK3CA: 5’-AUG CAU GCU GUU UAA UUG UG-3’, EMX1: 5’-GAG UCC GAG CAG AAG AAG AA-3’, FANCF: 5’-GGA AUC CCU UCU GCA GCA CC-3’). ..

    Expressing:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Article Title: The spindle-associated antisense RNA ARHGEF17-AS1 functionally contributes to mitotic spindle integrity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides siRNAs and LNA antisense oligonucleotides used in this study Sigma-Aldrich; Qiagen; this paper See Table S1 qPCR and ChIRP-qPCR primers used in this study This paper See Table S2 sgRNA and shRNA oligonucleotides used in this study Sigma-Aldrich; this paper See Table S3 ChIRP and ChOP capture probes used in this study Sigma-Aldrich; this paper See Table S4 ARHGEF17-AS1 knockout sequencing primers This paper See Table S5 Custom RNAscope probe set targeting human ARHGEF17-AS1 This paper/Advanced Cell Diagnostics/Bio-Techne Custom Probe design targeting second and third exon of AS1. .. Recombinant DNA pcDNA3.1+ mammalian expression vector Invitrogen Cat# V79020 pcDNA3.1-ARHGEF17-AS1 This paper N/A pLKO-Tet-On-shRNA-Control Junqueira Alves et al.29; Addgene Addgene plasmid #98398; RRID: Addgene_98398 pLKO.1-ARHGEF17-AS1-sh1 This paper N/A pLKO.1-ARHGEF17-AS1-sh2 This paper N/A pLVX-TetOne-Puro vector Takara Bio Cat# 631847 pLVX-TetOne-ARHGEF17-AS1 This paper N/A pLVX-TetOne-LacZ This paper N/A pLenti_Empty (EVC_Flag) Gift from Thomas Tuschl N/A IC74-mfGFP cytoplasmic dynein intermediate-chain expression plasmid Gift from Takashi Murayama; Kobayashi and Murayama30 N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.31; Addgene Addgene plasmid #48138; RRID: Addgene_48138 pSpCas9(BB)-2A-GFP (PX458)-sg1-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg1ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg3ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro Stringer et al.32; Addgene Addgene plasmid #98290; RRID: Addgene_98290 lentiCRISPRv2 puro-LacZ This paper N/A lentiCRISPRv2 puro-sg1-ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro-sg2-ARHGEF17-AS1 This paper N/A pLENTI_RPS3 Meyer et al.33; Addgene Addgene plasmid #127128; RRID: Addgene_127128 pLENTI_RPS3_K214R Meyer et al.33; Addgene Addgene plasmid #127132; RRID: Addgene_127132 pLENTI_RPS3_K227R Meyer et al.33; Addgene Addgene plasmid #127133; RRID: Addgene_127133 pLENTI_RPS3_K230R Meyer et al.33; Addgene Addgene plasmid #127134; RRID: Addgene_127134 scramble shRNA Sarbassov et al.34; Addgene Addgene plasmid #1864; RRID: Addgene_1864 (Continued on next page) 18 Cell Reports 45, 118023, October 27, 2026 .. HeLa (Homo sapiens; cervical adenocarcinoma; 31-year-old female donor; ATCC CCL-2) and A549 (Homo sapiens; lung adenocarcinoma; 58-year-old male donor; ECACC 86012804) cells were obtained from ATCC and The European Collection of Authenticated Cell Cultures (ECACC), respectively.

    Control:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Single Cell:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Cloning:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Biomarker Discovery:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Western Blot:

    Article Title: Antagonistic roles of histone H3 lysine methyltransferases dictate tumor immune surveillance
    Article Snippet: .. KP-Nsd2KO cell lines were generated by transducing LG1233 cells with lentiCRISPRv2Puro (Addgene 98290) expressing an sgRNA targeting Nsd2 or βgal (as a control), followed by single-cell cloning (described above), and validation of NSD2 loss by immunoblotting. .. KP-H3K36MSetd2KO-EZH2KO cell lines were produced using lentiCRISPRv2mCherry (Addgene 99154) with sgRNAs targeting either EZH2 or βgal (as a control) and transducing KP-H3K36M-Setd2KO cells with these constructs.

    Recombinant:

    Article Title: The spindle-associated antisense RNA ARHGEF17-AS1 functionally contributes to mitotic spindle integrity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides siRNAs and LNA antisense oligonucleotides used in this study Sigma-Aldrich; Qiagen; this paper See Table S1 qPCR and ChIRP-qPCR primers used in this study This paper See Table S2 sgRNA and shRNA oligonucleotides used in this study Sigma-Aldrich; this paper See Table S3 ChIRP and ChOP capture probes used in this study Sigma-Aldrich; this paper See Table S4 ARHGEF17-AS1 knockout sequencing primers This paper See Table S5 Custom RNAscope probe set targeting human ARHGEF17-AS1 This paper/Advanced Cell Diagnostics/Bio-Techne Custom Probe design targeting second and third exon of AS1. .. Recombinant DNA pcDNA3.1+ mammalian expression vector Invitrogen Cat# V79020 pcDNA3.1-ARHGEF17-AS1 This paper N/A pLKO-Tet-On-shRNA-Control Junqueira Alves et al.29; Addgene Addgene plasmid #98398; RRID: Addgene_98398 pLKO.1-ARHGEF17-AS1-sh1 This paper N/A pLKO.1-ARHGEF17-AS1-sh2 This paper N/A pLVX-TetOne-Puro vector Takara Bio Cat# 631847 pLVX-TetOne-ARHGEF17-AS1 This paper N/A pLVX-TetOne-LacZ This paper N/A pLenti_Empty (EVC_Flag) Gift from Thomas Tuschl N/A IC74-mfGFP cytoplasmic dynein intermediate-chain expression plasmid Gift from Takashi Murayama; Kobayashi and Murayama30 N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.31; Addgene Addgene plasmid #48138; RRID: Addgene_48138 pSpCas9(BB)-2A-GFP (PX458)-sg1-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg1ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg3ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro Stringer et al.32; Addgene Addgene plasmid #98290; RRID: Addgene_98290 lentiCRISPRv2 puro-LacZ This paper N/A lentiCRISPRv2 puro-sg1-ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro-sg2-ARHGEF17-AS1 This paper N/A pLENTI_RPS3 Meyer et al.33; Addgene Addgene plasmid #127128; RRID: Addgene_127128 pLENTI_RPS3_K214R Meyer et al.33; Addgene Addgene plasmid #127132; RRID: Addgene_127132 pLENTI_RPS3_K227R Meyer et al.33; Addgene Addgene plasmid #127133; RRID: Addgene_127133 pLENTI_RPS3_K230R Meyer et al.33; Addgene Addgene plasmid #127134; RRID: Addgene_127134 scramble shRNA Sarbassov et al.34; Addgene Addgene plasmid #1864; RRID: Addgene_1864 (Continued on next page) 18 Cell Reports 45, 118023, October 27, 2026 .. HeLa (Homo sapiens; cervical adenocarcinoma; 31-year-old female donor; ATCC CCL-2) and A549 (Homo sapiens; lung adenocarcinoma; 58-year-old male donor; ECACC 86012804) cells were obtained from ATCC and The European Collection of Authenticated Cell Cultures (ECACC), respectively.

    Plasmid Preparation:

    Article Title: The spindle-associated antisense RNA ARHGEF17-AS1 functionally contributes to mitotic spindle integrity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides siRNAs and LNA antisense oligonucleotides used in this study Sigma-Aldrich; Qiagen; this paper See Table S1 qPCR and ChIRP-qPCR primers used in this study This paper See Table S2 sgRNA and shRNA oligonucleotides used in this study Sigma-Aldrich; this paper See Table S3 ChIRP and ChOP capture probes used in this study Sigma-Aldrich; this paper See Table S4 ARHGEF17-AS1 knockout sequencing primers This paper See Table S5 Custom RNAscope probe set targeting human ARHGEF17-AS1 This paper/Advanced Cell Diagnostics/Bio-Techne Custom Probe design targeting second and third exon of AS1. .. Recombinant DNA pcDNA3.1+ mammalian expression vector Invitrogen Cat# V79020 pcDNA3.1-ARHGEF17-AS1 This paper N/A pLKO-Tet-On-shRNA-Control Junqueira Alves et al.29; Addgene Addgene plasmid #98398; RRID: Addgene_98398 pLKO.1-ARHGEF17-AS1-sh1 This paper N/A pLKO.1-ARHGEF17-AS1-sh2 This paper N/A pLVX-TetOne-Puro vector Takara Bio Cat# 631847 pLVX-TetOne-ARHGEF17-AS1 This paper N/A pLVX-TetOne-LacZ This paper N/A pLenti_Empty (EVC_Flag) Gift from Thomas Tuschl N/A IC74-mfGFP cytoplasmic dynein intermediate-chain expression plasmid Gift from Takashi Murayama; Kobayashi and Murayama30 N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.31; Addgene Addgene plasmid #48138; RRID: Addgene_48138 pSpCas9(BB)-2A-GFP (PX458)-sg1-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg1ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg3ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro Stringer et al.32; Addgene Addgene plasmid #98290; RRID: Addgene_98290 lentiCRISPRv2 puro-LacZ This paper N/A lentiCRISPRv2 puro-sg1-ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro-sg2-ARHGEF17-AS1 This paper N/A pLENTI_RPS3 Meyer et al.33; Addgene Addgene plasmid #127128; RRID: Addgene_127128 pLENTI_RPS3_K214R Meyer et al.33; Addgene Addgene plasmid #127132; RRID: Addgene_127132 pLENTI_RPS3_K227R Meyer et al.33; Addgene Addgene plasmid #127133; RRID: Addgene_127133 pLENTI_RPS3_K230R Meyer et al.33; Addgene Addgene plasmid #127134; RRID: Addgene_127134 scramble shRNA Sarbassov et al.34; Addgene Addgene plasmid #1864; RRID: Addgene_1864 (Continued on next page) 18 Cell Reports 45, 118023, October 27, 2026 .. HeLa (Homo sapiens; cervical adenocarcinoma; 31-year-old female donor; ATCC CCL-2) and A549 (Homo sapiens; lung adenocarcinoma; 58-year-old male donor; ECACC 86012804) cells were obtained from ATCC and The European Collection of Authenticated Cell Cultures (ECACC), respectively.

    Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways
    Article Snippet: .. The oligos were annealed and ligated into the lentiCRISPRv2-puro vector (Addgene_98290; Addgene, Inc.), and digested with Bsm BI according to the protocol provided by the Zhang lab ( https://www.addgene.org/crispr/zhang/ ). ..

    shRNA:

    Article Title: The spindle-associated antisense RNA ARHGEF17-AS1 functionally contributes to mitotic spindle integrity.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Oligonucleotides siRNAs and LNA antisense oligonucleotides used in this study Sigma-Aldrich; Qiagen; this paper See Table S1 qPCR and ChIRP-qPCR primers used in this study This paper See Table S2 sgRNA and shRNA oligonucleotides used in this study Sigma-Aldrich; this paper See Table S3 ChIRP and ChOP capture probes used in this study Sigma-Aldrich; this paper See Table S4 ARHGEF17-AS1 knockout sequencing primers This paper See Table S5 Custom RNAscope probe set targeting human ARHGEF17-AS1 This paper/Advanced Cell Diagnostics/Bio-Techne Custom Probe design targeting second and third exon of AS1. .. Recombinant DNA pcDNA3.1+ mammalian expression vector Invitrogen Cat# V79020 pcDNA3.1-ARHGEF17-AS1 This paper N/A pLKO-Tet-On-shRNA-Control Junqueira Alves et al.29; Addgene Addgene plasmid #98398; RRID: Addgene_98398 pLKO.1-ARHGEF17-AS1-sh1 This paper N/A pLKO.1-ARHGEF17-AS1-sh2 This paper N/A pLVX-TetOne-Puro vector Takara Bio Cat# 631847 pLVX-TetOne-ARHGEF17-AS1 This paper N/A pLVX-TetOne-LacZ This paper N/A pLenti_Empty (EVC_Flag) Gift from Thomas Tuschl N/A IC74-mfGFP cytoplasmic dynein intermediate-chain expression plasmid Gift from Takashi Murayama; Kobayashi and Murayama30 N/A pSpCas9(BB)-2A-GFP (PX458) Ran et al.31; Addgene Addgene plasmid #48138; RRID: Addgene_48138 pSpCas9(BB)-2A-GFP (PX458)-sg1-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2-LacZ This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg1ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg2ARHGEF17-AS1 This paper N/A pSpCas9(BB)-2A-GFP (PX458)-sg3ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro Stringer et al.32; Addgene Addgene plasmid #98290; RRID: Addgene_98290 lentiCRISPRv2 puro-LacZ This paper N/A lentiCRISPRv2 puro-sg1-ARHGEF17-AS1 This paper N/A lentiCRISPRv2 puro-sg2-ARHGEF17-AS1 This paper N/A pLENTI_RPS3 Meyer et al.33; Addgene Addgene plasmid #127128; RRID: Addgene_127128 pLENTI_RPS3_K214R Meyer et al.33; Addgene Addgene plasmid #127132; RRID: Addgene_127132 pLENTI_RPS3_K227R Meyer et al.33; Addgene Addgene plasmid #127133; RRID: Addgene_127133 pLENTI_RPS3_K230R Meyer et al.33; Addgene Addgene plasmid #127134; RRID: Addgene_127134 scramble shRNA Sarbassov et al.34; Addgene Addgene plasmid #1864; RRID: Addgene_1864 (Continued on next page) 18 Cell Reports 45, 118023, October 27, 2026 .. HeLa (Homo sapiens; cervical adenocarcinoma; 31-year-old female donor; ATCC CCL-2) and A549 (Homo sapiens; lung adenocarcinoma; 58-year-old male donor; ECACC 86012804) cells were obtained from ATCC and The European Collection of Authenticated Cell Cultures (ECACC), respectively.

    CRISPR:

    Article Title: Splicing-guaranteed artificial introns enable efficient homology-directed repair at internal exons and streamlined cell engineering
    Article Snippet: .. DSBs were generated by CRISPR–Cas9 technology with LentiCRISPR-V2 (Addgene, #98290) carrying the indicated intron-targeting sgRNAs (RELA: 5’-UCA CUG AGC UCC CGG UCG GA-3’, EGFR: 5’-GCU GGC CAA ACU GCU GGG UG-3’, PIK3CA: 5’-AUG CAU GCU GUU UAA UUG UG-3’, EMX1: 5’-GAG UCC GAG CAG AAG AAG AA-3’, FANCF: 5’-GGA AUC CCU UCU GCA GCA CC-3’). ..

    Cellular Antioxidant Activity Assay:

    Article Title: Splicing-guaranteed artificial introns enable efficient homology-directed repair at internal exons and streamlined cell engineering
    Article Snippet: .. DSBs were generated by CRISPR–Cas9 technology with LentiCRISPR-V2 (Addgene, #98290) carrying the indicated intron-targeting sgRNAs (RELA: 5’-UCA CUG AGC UCC CGG UCG GA-3’, EGFR: 5’-GCU GGC CAA ACU GCU GGG UG-3’, PIK3CA: 5’-AUG CAU GCU GUU UAA UUG UG-3’, EMX1: 5’-GAG UCC GAG CAG AAG AAG AA-3’, FANCF: 5’-GGA AUC CCU UCU GCA GCA CC-3’). ..

    Clone Assay:

    Article Title: CD112 Lactylation Drives Dual Immune Evasion From CD8+ T Cells and NK Cells.
    Article Snippet: .. sgRNAs targeting human AARS2 , human CD112 , human UBE3C , mouse Aars1 , mouse Aars2 , and mouse Cd112 were cloned into BsmBI-digested lentiCRISPRv2-puro or lentiCRISPRv2-blast vectors (Addgene). ..

    Sequencing:

    Article Title: Depleting HIF-1α attenuates the progression of osteosarcoma, but tumorigenicity is sustained through HIF-independent pathways
    Article Snippet: .. After confirmation of the inserted sequence, the lentiCRISPRv2-puro vectors were co-transfected along with the psPAX2 (cat. no. 12260; Addgene, Inc.) and pCMV–VSV-G (Addgene_8454; Addgene, Inc.) vectors into Lenti-X 293T cells (CVCL_4401; Takara Bio) using FuGENE HD (Promega Corporation) to generate infectious lentivirus. ..



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    Image Search Results


    Journal: bioRxiv

    Article Title: CANVAS causing AAGGG repeat expansions cause tissue-specific reduction in RFC1 expression and increase sensitivity to DNA damage

    doi: 10.1101/2025.11.18.688292

    Figure Lengend Snippet:

    Article Snippet: Briefly, Firefly Luciferase plasmid and the repeat containing pBluescript vectors were digested with XbaI and NotI restriction enzymes (NEB), while pGint plasmid and pBluescript vectors were digested with NotI and BamHI restriction enzymes (NEB) at 37°C for 1 hour.

    Techniques: Luciferase