custom vector (Addgene inc)
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Addgene inc
custom vector
Custom Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+vector/SpCas9_sgRNA_expression_in_pBluescript+(Plasmid+%23122089)/pm40725481-48-43-45
Average 93 stars, based on 12 article reviews
Custom Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pbluescript+vector/SpCas9_sgRNA_expression_in_pBluescript+(Plasmid+%23122089)/pm40725481-48-43-45
Average 93 stars, based on 12 article reviews
custom vector - by Bioz Stars,
2026-10
93/100 stars
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other:Article Title: The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles Article Snippet: The nucleotide sequence of both fusion proteins ( ) was cloned into psPAX2 via SphI and XcmI sites instead of the original sequence. psgRNA-MmCas12m and psgRNA-dSpCas9 encode specific single-guide RNA under the control of the U6 promoter. psgRNA-MmCas12m and psgRNA-dSpCas9 were obtained based on a donor Article Title: The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles Article Snippet: The nucleotide sequence of both fusion proteins (Supplementary Figures S3 and S4) was cloned into psPAX2 via SphI and XcmI sites instead of the original sequence. psgRNA-MmCas12m and psgRNA-dSpCas9 encode specific single-guide RNA under the control of the U6 promoter. psgRNA-MmCas12m and psgRNA-dSpCas9 were obtained based on a donor Expressing:Article Title: Detecting Methylation Changes Induced by Prime Editing Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: Detecting Methylation Changes Induced by Prime Editing. Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo Article Snippet: .. Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold sequence (for sgRNAs) or a 3′ direct repeat (DR)-spacer sequence (for crRNAs) were cloned into Article Title: The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles Article Snippet: dSpCas9 (#201953, Addgene) , Mammalian expression plasmid of catalytically inactive SpCas9. .. Polymerase Chain Reaction:Article Title: Detecting Methylation Changes Induced by Prime Editing Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: Detecting Methylation Changes Induced by Prime Editing. Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Amplification:Article Title: Detecting Methylation Changes Induced by Prime Editing Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: Detecting Methylation Changes Induced by Prime Editing. Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Clone Assay:Article Title: Detecting Methylation Changes Induced by Prime Editing Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: Detecting Methylation Changes Induced by Prime Editing. Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo Article Snippet: .. Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold sequence (for sgRNAs) or a 3′ direct repeat (DR)-spacer sequence (for crRNAs) were cloned into Article Title: Enhancing prime editing in hematopoietic stem and progenitor cells by modulating nucleotide metabolism. Article Snippet: Therapeutic prime editing of hematopoietic stem and progenitor cells (HSPCs) holds great potential to remedy blood disorders.. Quiescent cells have low nucleotide levels and resist retroviral infection, and it is possible that nucleotide metabolism could limit reverse transcription-mediated prime editing in HSPCs.. We demonstrate that deoxynucleoside supplementation and Vpx-mediated degradation of SAMHD1 improve prime editing efficiency in HSPCs, especially when coupled with editing approaches that evade mismatch repair. Plasmid Preparation:Article Title: Detecting Methylation Changes Induced by Prime Editing Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Article Title: Detecting Methylation Changes Induced by Prime Editing. Article Snippet: .. To generate pegRNA expression plasmids, gblocks or PCR products including spacer sequences, scaffold sequences, and 3′ extension sequences (pegRNA products from IDT) were amplified with indicated primers using Phusion master mix (Thermo Fisher Scientific, Waltham, MA, USA), which were subsequently cloned into a Sequencing:Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo Article Snippet: .. Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold sequence (for sgRNAs) or a 3′ direct repeat (DR)-spacer sequence (for crRNAs) were cloned into Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into Molecular Cloning:Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into Construct:Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo Article Snippet: .. 86 MATERIALS AND METHODS 87 General methods and molecular cloning 88 Expression plasmids used for sgRNAs or crRNAs were constructed based on previously 89 described backbones.35 Briefly, gblocks (ordered from GenScript) containing a spacer-scaffold 90 sequence (for sgRNAs) or a 3’ Direct Repeat (DR)-spacer sequence (for crRNAs) were cloned 91 into Cloning:Article Title: The Disruption of the HIV-1 Gag Start Codon via Editing Using MmCas12m-Dual Base Editor-Loaded Virus-like Particles Article Snippet: dSpCas9 (#201953, Addgene) , Mammalian expression plasmid of catalytically inactive SpCas9. .. |