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GenTel BioSurfaces path protein microarray slides
Path Protein Microarray Slides, supplied by GenTel BioSurfaces, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/path+microarray+slides/chip+based+proteomic+protein+microarray/us11207381-317-38-42
Average 90 stars, based on 1 article reviews
path protein microarray slides - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Diagnostic Assay:

Article Title: Protein microarrays as an application for disease biomarkers.
Article Snippet: Protein microarrays are an increasingly powerful technology in the hunt for new and novel diagnostic and prognostic biomarkers.. Lending credit to the highly established DNA microarray, protein microarrays are versatile tools that utilize a variety of formats to facilitate the discovery of new biomarkers and our understanding of disease pathways.. The aims of this review are: to detail a variety of protein microarray technologies currently used, including forward-phase technologies and reverse-phase technologies useful in both the discovery and validation of candidate biomarkers; to explore the strengths and weaknesses of various proteomic microarray platforms; to explain how bioinformatics helps compare data between microarray data sets; and to discuss the downstream applications of such technologies as they relate to the development of a highly personalized approach to medicine.

Control:

Article Title: Cell-free Determination of Binary Complexes That Comprise Extended Protein-Protein Interaction Networks of Yersinia pestis
Article Snippet: The Y. pestis proteins were expressed in vitro using Expressway TM (Life Technologies) cell-free E. coli extract system and isolated by GST-based affinity purification. .. Proteins that passed quality control criteria of quantity, solubility, and stability (3,552 proteins out of 4,146 total ORFs), representing ~85% total proteome coverage, were printed in microarrays on glass slides coated with a thin film of nitrocellulose (Gentel Biosciences, Madison, WI). ..

Article Title: Cytokine receptor peptides, compositions thereof and methods thereof
Article Snippet: Amplified stock of M13 phage control (M13KEgIII Cloning Vector, New England Biolabs, Cat. No. E8101S) was stored in Tris Buffered Saline (TBS; pH7.5, 4° C.) and diluted with Print Reagent to the working titre (108 pfu/μl). .. An automated spotting robot (16 pin print tool, 0.4 mm head; BioRobotics, UK) was used to print the CK/BSA control/M13 control, 5 repeats per sample (CK 25 μg/ml and 50 μg/ml; BSA 100 μg/ml; M13 20 μl), on PATH Protein Microarray Slides (GenTel, Prod. ..

Article Title: Cytokine receptor peptides, compositions thereof and methods thereof
Article Snippet: Amplified stock of M13 phage control (M13KEgIII Cloning Vector, New England Biolabs, Cat. No. E8101S) was stored in Tris Buffered Saline (TBS; pH7.5, 4° C.) and diluted with Print Reagent to the working titre (108 pfu/μ1). .. An automated spotting robot (16 pin print tool, 0.4 mm head; BioRobotics, UK) was used to print the CK/BSA control/M13 control, 5 repeats per sample (CK 25 μg/ml and 50 μg/ml; BSA 100 μg/ml; M13 20 μl), on PATH Protein Microarray Slides (GenTel, Prod. ..

Solubility:

Article Title: Cell-free Determination of Binary Complexes That Comprise Extended Protein-Protein Interaction Networks of Yersinia pestis
Article Snippet: The Y. pestis proteins were expressed in vitro using Expressway TM (Life Technologies) cell-free E. coli extract system and isolated by GST-based affinity purification. .. Proteins that passed quality control criteria of quantity, solubility, and stability (3,552 proteins out of 4,146 total ORFs), representing ~85% total proteome coverage, were printed in microarrays on glass slides coated with a thin film of nitrocellulose (Gentel Biosciences, Madison, WI). ..

other:

Article Title: Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions.
Article Snippet: AVM BIOMED microarray system containing 21, 065 human recombinant proteins onto thin-film nitrocellulose-coated slides were prepared as described previously51 by GenTel’s Biosciences (Madison, WI).

Article Title: Ubiquitin ligase CHFR mediated degradation of VE-cadherin through ubiquitylation disrupts endothelial adherens junctions
Article Snippet: AVM BIOMED microarray system containing 21, 065 human recombinant proteins onto thin-film nitrocellulose-coated slides were prepared as described previously by GenTel’s Biosciences (Madison, WI).

Microarray:

Article Title: Cytokine receptor peptides, compositions thereof and methods thereof
Article Snippet: Amplified stock of M13 phage control (M13KEgIII Cloning Vector, New England Biolabs, Cat. No. E8101S) was stored in Tris Buffered Saline (TBS; pH7.5, 4° C.) and diluted with Print Reagent to the working titre (108 pfu/μl). .. An automated spotting robot (16 pin print tool, 0.4 mm head; BioRobotics, UK) was used to print the CK/BSA control/M13 control, 5 repeats per sample (CK 25 μg/ml and 50 μg/ml; BSA 100 μg/ml; M13 20 μl), on PATH Protein Microarray Slides (GenTel, Prod. ..

Article Title: Cytokine receptor peptides, compositions thereof and methods thereof
Article Snippet: Amplified stock of M13 phage control (M13KEgIII Cloning Vector, New England Biolabs, Cat. No. E8101S) was stored in Tris Buffered Saline (TBS; pH7.5, 4° C.) and diluted with Print Reagent to the working titre (108 pfu/μ1). .. An automated spotting robot (16 pin print tool, 0.4 mm head; BioRobotics, UK) was used to print the CK/BSA control/M13 control, 5 repeats per sample (CK 25 μg/ml and 50 μg/ml; BSA 100 μg/ml; M13 20 μl), on PATH Protein Microarray Slides (GenTel, Prod. ..

Article Title: Reagents and methods for treating cancer
Article Snippet: .. Homogenates of tumor tissue (left) and adjacent normal tissue (right) are spotted onto a polyvinyldifluoride membrane in triplicate in SomaPlexTM Cancer Tissue Lysate Protein Microarray Slides (Gentel, Madison, Wis.). .. The array was probed with an anti-EVI1 monoclonal antibody purchased from Cell Signaling.

Ab Array:

Article Title: Protein microarrays as an application for disease biomarkers.
Article Snippet: Protein microarrays are an increasingly powerful technology in the hunt for new and novel diagnostic and prognostic biomarkers.. Lending credit to the highly established DNA microarray, protein microarrays are versatile tools that utilize a variety of formats to facilitate the discovery of new biomarkers and our understanding of disease pathways.. The aims of this review are: to detail a variety of protein microarray technologies currently used, including forward-phase technologies and reverse-phase technologies useful in both the discovery and validation of candidate biomarkers; to explore the strengths and weaknesses of various proteomic microarray platforms; to explain how bioinformatics helps compare data between microarray data sets; and to discuss the downstream applications of such technologies as they relate to the development of a highly personalized approach to medicine.

Biomarker Discovery:

Article Title: Protein microarrays as an application for disease biomarkers.
Article Snippet: Protein microarrays are an increasingly powerful technology in the hunt for new and novel diagnostic and prognostic biomarkers.. Lending credit to the highly established DNA microarray, protein microarrays are versatile tools that utilize a variety of formats to facilitate the discovery of new biomarkers and our understanding of disease pathways.. The aims of this review are: to detail a variety of protein microarray technologies currently used, including forward-phase technologies and reverse-phase technologies useful in both the discovery and validation of candidate biomarkers; to explore the strengths and weaknesses of various proteomic microarray platforms; to explain how bioinformatics helps compare data between microarray data sets; and to discuss the downstream applications of such technologies as they relate to the development of a highly personalized approach to medicine.

Membrane:

Article Title: Reagents and methods for treating cancer
Article Snippet: .. Homogenates of tumor tissue (left) and adjacent normal tissue (right) are spotted onto a polyvinyldifluoride membrane in triplicate in SomaPlexTM Cancer Tissue Lysate Protein Microarray Slides (Gentel, Madison, Wis.). .. The array was probed with an anti-EVI1 monoclonal antibody purchased from Cell Signaling.



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Grace Bio-Labs path protein microarray slides
Construction and characterization of the ASFV proteome <t>microarray.</t> In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a <t>PATH</t> substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.
Path Protein Microarray Slides, supplied by Grace Bio-Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Construction and characterization of the ASFV proteome <t>microarray.</t> In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a <t>PATH</t> substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.
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path protein microarray slides - by Bioz Stars, 2026-09
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Construction and characterization of the ASFV proteome <t>microarray.</t> In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a <t>PATH</t> substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.
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Construction and characterization of the ASFV proteome <t>microarray.</t> In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a <t>PATH</t> substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.
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Fisher Scientific path microarray slides
Construction and characterization of the ASFV proteome <t>microarray.</t> In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a <t>PATH</t> substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.
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GenTel BioSurfaces high binding capacity microarray slides path

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Image Search Results


Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.

Journal: Journal of Virology

Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

doi: 10.1128/jvi.00478-25

Figure Lengend Snippet: Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.

Article Snippet: Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on PATH Protein Microarray Slides (GraceBio-Labs, Oregon, USA).

Techniques: Microarray, Labeling, Fluorescence, Infection

High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.

Journal: Journal of Virology

Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

doi: 10.1128/jvi.00478-25

Figure Lengend Snippet: High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.

Article Snippet: Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on PATH Protein Microarray Slides (GraceBio-Labs, Oregon, USA).

Techniques: High Throughput Screening Assay, Infection, Microarray, Virus

Journal: Journal of Visualized Experiments : JoVE

Article Title: Chemically-blocked Antibody Microarray for Multiplexed High-throughput Profiling of Specific Protein Glycosylation in Complex Samples

doi: 10.3791/3791

Figure Lengend Snippet:

Article Snippet: High binding capacity microarray slides, such as PATH (Gentel Bio Inc. WI) or Slide H (Shott, PA).

Techniques: Microarray, Staining, Incubation, Saline, Protease Inhibitor