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patch clamp recordings  (Danaher Inc)


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    Danaher Inc patch clamp recordings
    Patch Clamp Recordings, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1364 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/patch-clamp+recordings/MultiClamp+700B+current+%26+patch+clamp+amplifier/pm41579371-252-1-10
    Average 99 stars, based on 1364 article reviews
    patch clamp recordings - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Amplification:

    Article Title: Two-photon voltage imaging with rhodopsin-based sensors.
    Article Snippet: .. Signals were amplified (MultiClamp 700B, Molecular Devices), filtered at 2/6/10 kHz and digitised at 10/50/500 kHz (DigiData 1400 or 1550B, Molecular Devices) using the Clampex software (Version 10.6 and 11.1). ..

    Article Title: Whole-Brain Co-Mapping of Gene Expression and Neuronal Activity at Cellular Resolution in Behaving Zebrafish
    Article Snippet: Agar was cleared from part of the tail and two suction borosillicate pipettes (∼60 μm inner diameter, TW150-3, World Precision Instruments) were attached to the skin by gentle suction on each side of the tail. .. Extracellular motor nerve signals were amplified (Molecular Devices, Axon Multiclamp 700B) in current clamp and recorded with a custom written C# software. ..

    Article Title: Juvenile-to-adult refinement of thalamic reticular circuits via LRRTM3 enables high-resolution sensory encoding.
    Article Snippet: In brief Contrary to the classical view that sensory circuits stabilize after early critical periods, Lee, Han, et al. demonstrate that the thalamic reticular nucleus (TRN) continues to undergo functionally significant remodeling into adulthood.. This late-stage refinement, mediated by the synaptic adhesion molecule LRRTM3, selectively rebalances corticothalamic inputs to the TRN via distinct extracellular mechanisms.. LRRTM3-dependent circuit maturation is essential for achieving adult-like perceptual precision.

    Article Title: A dorsal hippocampus-prodynorphinergic dorsolateral septum-to-lateral hypothalamus circuit mediates contextual gating of feeding.
    Article Snippet: In brief Goode et al. show that prodynorphin (Pdyn)-expressing neurons of the dorsolateral septum (DLS) receive substantial dorsal hippocampal (DHPC) input and inhibit lateral hypothalamic (LHA) GABAergic neurons.. DLS(Pdyn) activity is changed by training for food, is context-sensitive, and its disruption by optogenetics (or Pdyn deletion) in DHPCDLS(Pdyn)-LHA circuits impairs conditioned feeding.

    Software:

    Article Title: Two-photon voltage imaging with rhodopsin-based sensors.
    Article Snippet: .. Signals were amplified (MultiClamp 700B, Molecular Devices), filtered at 2/6/10 kHz and digitised at 10/50/500 kHz (DigiData 1400 or 1550B, Molecular Devices) using the Clampex software (Version 10.6 and 11.1). ..

    Article Title: Piezo1 specific deletion in endothelial cell protects the progression of pulmonary fibrosis in mice.
    Article Snippet: .. An Axon Multiclamp 700B amplifier (Molecular Devices) was used to amplify the signals, which were then low-pass filtered at 1 kHz and sampled at 3 kHz via a Digidata 1440A digitizer. pCLAMP software (Molecular Devices) was employed for data acquisition and subsequent analysis. ..

    Article Title: Whole-Brain Co-Mapping of Gene Expression and Neuronal Activity at Cellular Resolution in Behaving Zebrafish
    Article Snippet: Agar was cleared from part of the tail and two suction borosillicate pipettes (∼60 μm inner diameter, TW150-3, World Precision Instruments) were attached to the skin by gentle suction on each side of the tail. .. Extracellular motor nerve signals were amplified (Molecular Devices, Axon Multiclamp 700B) in current clamp and recorded with a custom written C# software. ..

    Article Title: Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse models.
    Article Snippet: Fire-polished borosilicate glass capillaries (Sutter Instrument, Cat #BF150-75-10) were pulled (tip resistance of about 9–12 MΩ) using a P1000 pipette (Sutter Instrument, Novato, CA, United States) and filled with an internal solution containing (in mM): 130 K-gluconate, 6 KCl, 4 NaCl, 10 NaHEPES, 0.2 K-EGTA, 0.3 GTP, 2 Mg-ATP, 0.2 cAMP, 10 D-glucose, 0.15% biocytin and 0.06% rhodamine (pH 7.5, osmolarity adjusted to 290–300 mOsm). .. All measurements were done at room temperature using a patch clamp amplifier (MultiClamp 700B, Molecular Devices, San Jose, CA, United States), connected to a digitizer (Axon Digidata 1550B, Molecular Devices, San Jose, CA, United States), and controlled by MultiClamp 700B Commander and pCLAMP 11 software. .. Data were acquired at a sampling rate of 20 kHz and analyzed using Clampfit-10 and the software package MATLAB (release 2014b; The MathWorks, Natick, MA, USA).

    Article Title: Juvenile-to-adult refinement of thalamic reticular circuits via LRRTM3 enables high-resolution sensory encoding.
    Article Snippet: In brief Contrary to the classical view that sensory circuits stabilize after early critical periods, Lee, Han, et al. demonstrate that the thalamic reticular nucleus (TRN) continues to undergo functionally significant remodeling into adulthood.. This late-stage refinement, mediated by the synaptic adhesion molecule LRRTM3, selectively rebalances corticothalamic inputs to the TRN via distinct extracellular mechanisms.. LRRTM3-dependent circuit maturation is essential for achieving adult-like perceptual precision.

    Article Title: Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse models
    Article Snippet: Fire-polished borosilicate glass capillaries (Sutter Instrument, Cat #BF150-75-10) were pulled (tip resistance of ~9–12 MΩ) using a P1000 pipette (Sutter Instrument, Novato, CA, United States) and filled with an internal solution containing (in mM): 130 K-gluconate, 6 KCl, 4 NaCl, 10 Na-HEPES, 0.2 K-EGTA, 0.3 GTP, 2 Mg-ATP, 0.2 cAMP, 10 D-glucose (pH 7.5, osmolarity adjusted to 290–300 mOsm). .. All measurements were done at room temperature using a patch clamp amplifier (MultiClamp 700B, Molecular Devices, San Jose, CA, United States), connected to a digitizer (Axon Digidata 1550B, Molecular Devices, San Jose, CA, United States), and controlled by MultiClamp 700B Commander and pCLAMP 11 software. .. Data were acquired at a sampling rate of 20 kHz and analyzed using Clampfit-10 and the software package MATLAB (release 2014b; The MathWorks, Natick, MA, USA).

    Patch Clamp:

    Article Title: Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse models.
    Article Snippet: Fire-polished borosilicate glass capillaries (Sutter Instrument, Cat #BF150-75-10) were pulled (tip resistance of about 9–12 MΩ) using a P1000 pipette (Sutter Instrument, Novato, CA, United States) and filled with an internal solution containing (in mM): 130 K-gluconate, 6 KCl, 4 NaCl, 10 NaHEPES, 0.2 K-EGTA, 0.3 GTP, 2 Mg-ATP, 0.2 cAMP, 10 D-glucose, 0.15% biocytin and 0.06% rhodamine (pH 7.5, osmolarity adjusted to 290–300 mOsm). .. All measurements were done at room temperature using a patch clamp amplifier (MultiClamp 700B, Molecular Devices, San Jose, CA, United States), connected to a digitizer (Axon Digidata 1550B, Molecular Devices, San Jose, CA, United States), and controlled by MultiClamp 700B Commander and pCLAMP 11 software. .. Data were acquired at a sampling rate of 20 kHz and analyzed using Clampfit-10 and the software package MATLAB (release 2014b; The MathWorks, Natick, MA, USA).

    Article Title: Extracellular vesicles from stem cells rescue cellular phenotypes and behavioral deficits in SHANK3-associated ASD neuronal and mouse models
    Article Snippet: Fire-polished borosilicate glass capillaries (Sutter Instrument, Cat #BF150-75-10) were pulled (tip resistance of ~9–12 MΩ) using a P1000 pipette (Sutter Instrument, Novato, CA, United States) and filled with an internal solution containing (in mM): 130 K-gluconate, 6 KCl, 4 NaCl, 10 Na-HEPES, 0.2 K-EGTA, 0.3 GTP, 2 Mg-ATP, 0.2 cAMP, 10 D-glucose (pH 7.5, osmolarity adjusted to 290–300 mOsm). .. All measurements were done at room temperature using a patch clamp amplifier (MultiClamp 700B, Molecular Devices, San Jose, CA, United States), connected to a digitizer (Axon Digidata 1550B, Molecular Devices, San Jose, CA, United States), and controlled by MultiClamp 700B Commander and pCLAMP 11 software. .. Data were acquired at a sampling rate of 20 kHz and analyzed using Clampfit-10 and the software package MATLAB (release 2014b; The MathWorks, Natick, MA, USA).

    Article Title: A dorsal hippocampus-prodynorphinergic dorsolateral septum-to-lateral hypothalamus circuit mediates contextual gating of feeding.
    Article Snippet: In brief Goode et al. show that prodynorphin (Pdyn)-expressing neurons of the dorsolateral septum (DLS) receive substantial dorsal hippocampal (DHPC) input and inhibit lateral hypothalamic (LHA) GABAergic neurons.. DLS(Pdyn) activity is changed by training for food, is context-sensitive, and its disruption by optogenetics (or Pdyn deletion) in DHPCDLS(Pdyn)-LHA circuits impairs conditioned feeding.



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    A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole <t>cell</t> <t>patch</t> <t>clamp</t> <t>recordings,</t> antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).
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    Image Search Results


    A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

    Journal: bioRxiv

    Article Title: Sensory-motor integration in a nonspiking interneuron contributes to active sensor control in Drosophila

    doi: 10.64898/2026.04.16.718965

    Figure Lengend Snippet: A) Schematic depicting JONs projecting through the antennal nerve and synapsing onto APN2 cells (gold dashed box) in the ipsilateral hemisphere of the central brain. Inset shows expression pattern of the genetic driver line labeling APN2 ( 24C06-GAL4) . Scale bar is 20 µm. B) During whole cell patch clamp recordings, antennal movements were recorded by a lateral camera and flight activity was monitored using an optical wingbeat detector. C) Example video frame with 2 tracked points and the relative direction of antenna angle deflections. Deflections down towards the head are represented as negative values, and deflections up and away from the head as positive values. D) Example single-trial traces showing antennal and APN2 activity during quiescence (left) and flight (right). Wing movement was detected using an infrared light sensor. E) Each dot is the average response of one APN2 cell. Black bars indicate the average across APN2 cells for each condition, with respective SEM error bars. Grey dashed lines pair measurements from the same cell during flight and quiescence. Across flies, the average APN2 membrane potential is reduced during bouts of flight compared to quiescence, both when the antennae are free (paired t-test; p = 0.037) and when the antennae are glued (Wilcoxon Signed-Rank Test; p = 0.014).

    Article Snippet: We used 6-11 MΩ thick-walled glass pipettes (Item #1B150F-3, World Precision Instruments) for whole cell patch clamp recordings, which we pulled using a Sutter P-1000 puller.

    Techniques: Expressing, Labeling, Patch Clamp, Activity Assay, Membrane