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patch-clamp experiments
Patch Clamp Experiments, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patch+clamp+experiment/patch+clamp+experiments/pmc05851882-154-2-13
Average 90 stars, based on 1 article reviews
Patch Clamp Experiments, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/patch+clamp+experiment/patch+clamp+experiments/pmc05851882-154-2-13
Average 90 stars, based on 1 article reviews
patch-clamp experiments - by Bioz Stars,
2026-11
90/100 stars
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1) Product Images from "The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy"
Article Title: The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy
Journal: The Journal of Physiology
doi: 10.1113/JP274524
Figure Legend Snippet: qPCR analysis of mRNA levels from BKCa α‐ (A, n = 6–10), β1‐ (B, n = 5–7) and γ1‐subunits (C, n = 6–8) in UAs isolated from NP, P14 and P18 WT mice. mRNA expression was normalized to expression of succinate dehydrogenase complex flavoprotein subunit A. Columns are mean values ± SEM. † P < 0.05 compared to NP.
Techniques Used: Isolation, Expressing
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Patch Clamp:Article Title: The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy Article Snippet: In both cases, the MgCl 2 concentration was then raised to 10 m m for 30 min, permeabilization was reversed by placing the UAs in a MOPS‐buffered solution containing (in m m ): 140 NaCl, 5 KCl, 10 MgCl 2 , 5 glucose, and 2 MOPS (pH 7.1 with NaOH) at room temperature for 30 min, [Ca 2+ ] was gradually increased from nominally Ca 2+ ‐free to 0.01, 0.1 and 1.8 m m over a 45 min period, and then the UAs were incubated at 37°C in serum‐ and HEPES‐free Dulbecco's modified Eagle medium (DMEM; Invitrogen) for 48–60 h. Statistical analyses The numbers of pups per litter and data obtained from Doppler ultrasound and vascular reactivity experiments were subjected to two‐way ANOVA followed by Real-time Polymerase Chain Reaction:Article Title: The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy Article Snippet: In both cases, the MgCl 2 concentration was then raised to 10 m m for 30 min, permeabilization was reversed by placing the UAs in a MOPS‐buffered solution containing (in m m ): 140 NaCl, 5 KCl, 10 MgCl 2 , 5 glucose, and 2 MOPS (pH 7.1 with NaOH) at room temperature for 30 min, [Ca 2+ ] was gradually increased from nominally Ca 2+ ‐free to 0.01, 0.1 and 1.8 m m over a 45 min period, and then the UAs were incubated at 37°C in serum‐ and HEPES‐free Dulbecco's modified Eagle medium (DMEM; Invitrogen) for 48–60 h. Statistical analyses The numbers of pups per litter and data obtained from Doppler ultrasound and vascular reactivity experiments were subjected to two‐way ANOVA followed by Software:Article Title: The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy Article Snippet: In both cases, the MgCl 2 concentration was then raised to 10 m m for 30 min, permeabilization was reversed by placing the UAs in a MOPS‐buffered solution containing (in m m ): 140 NaCl, 5 KCl, 10 MgCl 2 , 5 glucose, and 2 MOPS (pH 7.1 with NaOH) at room temperature for 30 min, [Ca 2+ ] was gradually increased from nominally Ca 2+ ‐free to 0.01, 0.1 and 1.8 m m over a 45 min period, and then the UAs were incubated at 37°C in serum‐ and HEPES‐free Dulbecco's modified Eagle medium (DMEM; Invitrogen) for 48–60 h. Statistical analyses The numbers of pups per litter and data obtained from Doppler ultrasound and vascular reactivity experiments were subjected to two‐way ANOVA followed by Isolation:Article Title: The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy Article Snippet: In both cases, the MgCl 2 concentration was then raised to 10 m m for 30 min, permeabilization was reversed by placing the UAs in a MOPS‐buffered solution containing (in m m ): 140 NaCl, 5 KCl, 10 MgCl 2 , 5 glucose, and 2 MOPS (pH 7.1 with NaOH) at room temperature for 30 min, [Ca 2+ ] was gradually increased from nominally Ca 2+ ‐free to 0.01, 0.1 and 1.8 m m over a 45 min period, and then the UAs were incubated at 37°C in serum‐ and HEPES‐free Dulbecco's modified Eagle medium (DMEM; Invitrogen) for 48–60 h. Statistical analyses The numbers of pups per litter and data obtained from Doppler ultrasound and vascular reactivity experiments were subjected to two‐way ANOVA followed by Expressing:Article Title: The large‐conductance voltage‐ and Ca 2+ ‐activated K + channel and its γ1‐subunit modulate mouse uterine artery function during pregnancy Article Snippet: In both cases, the MgCl 2 concentration was then raised to 10 m m for 30 min, permeabilization was reversed by placing the UAs in a MOPS‐buffered solution containing (in m m ): 140 NaCl, 5 KCl, 10 MgCl 2 , 5 glucose, and 2 MOPS (pH 7.1 with NaOH) at room temperature for 30 min, [Ca 2+ ] was gradually increased from nominally Ca 2+ ‐free to 0.01, 0.1 and 1.8 m m over a 45 min period, and then the UAs were incubated at 37°C in serum‐ and HEPES‐free Dulbecco's modified Eagle medium (DMEM; Invitrogen) for 48–60 h. Statistical analyses The numbers of pups per litter and data obtained from Doppler ultrasound and vascular reactivity experiments were subjected to two‐way ANOVA followed by |