passive buffer (Promega)
90
Structured Review
Promega
passive buffer
Passive Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/passivation+buffer/passive+buffer/pmc06803248-331-23-25
Average 90 stars, based on 1 article reviews
Passive Buffer, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/passivation+buffer/passive+buffer/pmc06803248-331-23-25
Average 90 stars, based on 1 article reviews
passive buffer - by Bioz Stars,
2026-11
90/100 stars
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Incubation:Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. After incubation for 48 h, the transfected cells were lysed using Article Title: Eight-amino-acid sequence at the N-terminus of SARS-CoV-2 nsp1 is involved in stabilizing viral genome replication. Article Snippet: Coronavirus disease 19 is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) enveloped virus with a single-stranded positive-sense ribonucleic acid (RNA) genome.. The CoV non-structural protein (nsp) 1 is a multifunctional protein that undergoes translation shutoff, messenger RNA (mRNA) cleavage, and RNA binding.. The C-terminal region is involved in translational shutoff and RNA cleavage. Transfection:Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. After incubation for 48 h, the transfected cells were lysed using Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. Transfected cells were cultured for 24 and 48 h and then lysed with Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. At 24 h post-transfection, the transfected cells were lysed with Article Title: Eight-amino-acid sequence at the N-terminus of SARS-CoV-2 nsp1 is involved in stabilizing viral genome replication. Article Snippet: Coronavirus disease 19 is caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) enveloped virus with a single-stranded positive-sense ribonucleic acid (RNA) genome.. The CoV non-structural protein (nsp) 1 is a multifunctional protein that undergoes translation shutoff, messenger RNA (mRNA) cleavage, and RNA binding.. The C-terminal region is involved in translational shutoff and RNA cleavage. Article Title: Amino acid T25 in the substrate-binding domain of SARS-CoV-2 nsp5 is involved in viral replication in the mouse lung Article Snippet: The seeded cells were transfected with pGlo-VTFQS and nsp5 expression plasmids using the TransIT-LT1 Transfection Reagent (Mirus Bio) according to the manufacturer’s instructions. pCAGGS-MCS, a backbone for the nsp5 expression plasmid, was used as a control. .. Transfected cells were cultured for 48 h and then lysed with Reporter Assay:Article Title: Platycodin D induces apoptosis through JNK1/AP-1/PUMA pathway in non-small cell lung cancer cells: A new mechanism for an old compound Article Snippet: Cells (3 × 10 4 cells per well in a 24-well plate) were transfected with 0.2 μg of PUMA promoter luciferase reporter or 0.2 μg of 4X AP-1 luciferase reporter along with 10 ng of Renilla luciferase reporter (pRL-SV40) using PolyJet transfection reagent (SignaGen Laboratories). .. Cells were lysed in 1 × Cell Culture:Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. Transfected cells were cultured for 24 and 48 h and then lysed with Article Title: Amino acid T25 in the substrate-binding domain of SARS-CoV-2 nsp5 is involved in viral replication in the mouse lung Article Snippet: The seeded cells were transfected with pGlo-VTFQS and nsp5 expression plasmids using the TransIT-LT1 Transfection Reagent (Mirus Bio) according to the manufacturer’s instructions. pCAGGS-MCS, a backbone for the nsp5 expression plasmid, was used as a control. .. Transfected cells were cultured for 48 h and then lysed with Luciferase:Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. At 24 h post-transfection, the transfected cells were lysed with Activity Assay:Article Title: Regulation viral RNA transcription and replication by higher-order RNA structures within the nsp1 coding region of MERS coronavirus Article Snippet: .. At 24 h post-transfection, the transfected cells were lysed with Pull Down Assay:Article Title: Selective Proteolysis of Activated Transcriptional Factor by NIR-Responsive Palindromic DNA Thalidomide Conjugate Inhibits the Canonical Smad Pathway. Article Snippet: Dysfunctional transcription factors that activate abnormal expressions of specific proteins are often associated with the progression of various diseases.. Despite being attractive drug targets, the lack of druggable sites has dramatically hindered their drug development.. The emergence of proteolysis targeting chimeras (PROTACs) has revitalized the drug development of many conventional hard-to-drug protein targets. |