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Structured Review

Huabio Inc p62
P62, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p62/anti+p62/pm40407305-80-40-43
Average 86 stars, based on 1 article reviews
p62 - by Bioz Stars, 2026-09
86/100 stars

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Related Articles

Blocking Assay:

Article Title: Traditional Chinese Medicinal Leech Induces Apoptosis and Autophagy in Glioblastoma by SGK1/Caspase-3 and PI3K/AKT/mTOR Pathway.
Article Snippet: .. Following the blocking step, the membranes were maintained overnight at 4°C with the following antibodies: β- Actin (CST, #3700, 1:1000), GAPDH (Servicebio, GB11002, 1:1000), LC3B (Proteintech, 14,600- 1- AP, 1:1000), ATG5 (Huabio, ET611- 38, 1:1000), p62 (Huabio, HA721171, 1:2000), Beclin1 (Huabio, HA721216, 1:2000), AKT (CST, #9272, 1:1000), phospho- AKT (CST, 4060, 1:2000), mTOR (Abclonal, A2445, 1:2000), phospho- mTOR (Abclonal, AP0049, 1:2000), Caspase- 3 (CST, #14220, 1:1000), BCL- 2 (Huabio, ET702- 53, 1:2000), BAX (Abclonal, A19684, 1:2000), SGK1 (Proteintech, 28,454- 1- AP, 1:2000). ..

Western Blot:

Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy
Article Snippet: .. The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), p62 (HuaBio, HA721171), Beclin1 (CST, 3495S), LC3A/B (CST, 12741T), p-MLKL (MCE, HY-P81878), MLKL (HuaBio, ET1601-25), N-cadherin (Affinity, AF5239), E-cadherin (Affinity, AF0131), α-SMA (HuaBio, ET1607-53), Vimentin (Affinity, AF7013), Collegen-I (Proteintech, 66761-1-Ig), Collegen-III (Abcam, ab7778). ..

Immunofluorescence:

Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy
Article Snippet: .. The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), p62 (HuaBio, HA721171), Beclin1 (CST, 3495S), LC3A/B (CST, 12741T), p-MLKL (MCE, HY-P81878), MLKL (HuaBio, ET1601-25), N-cadherin (Affinity, AF5239), E-cadherin (Affinity, AF0131), α-SMA (HuaBio, ET1607-53), Vimentin (Affinity, AF7013), Collegen-I (Proteintech, 66761-1-Ig), Collegen-III (Abcam, ab7778). ..

Immunohistochemistry:

Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy
Article Snippet: .. The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), p62 (HuaBio, HA721171), Beclin1 (CST, 3495S), LC3A/B (CST, 12741T), p-MLKL (MCE, HY-P81878), MLKL (HuaBio, ET1601-25), N-cadherin (Affinity, AF5239), E-cadherin (Affinity, AF0131), α-SMA (HuaBio, ET1607-53), Vimentin (Affinity, AF7013), Collegen-I (Proteintech, 66761-1-Ig), Collegen-III (Abcam, ab7778). ..

other:

Article Title: Integrating network pharmacology and experimental validation to elucidate the mechanism by which 2-Dihydroailanthone induces HCT116 cell death through suppressing autophagy via the AMPK/mTOR pathway.
Article Snippet: Primary antibodies included AMPK (Cat# HA723038), p-AMPK (Ser496, Cat# ET1612-72), mTOR (Cat# ET1608-5), p-mTOR (Ser2448, Cat# HA600094), LC3B (Cat# ET1701-65), β-Actin (Cat# R1207-1), and p62 (Cat# HA721171) from HUABIO (China).

Incubation:

Article Title: USP38 regulates autophagy-dependent ferroptosis by deubiquitinating CTNNB1 in melanoma.
Article Snippet: The incidence of melanoma continues to rise gradually, and its clinical management remains challenging.. Ubiquitin-specific protease 38 (USP38) regulates tumor progression in multiple cancers, but its mechanism in melanoma remains elusive.. We discovered USP38 overexpression in human melanoma tissues.

Article Title: Preliminary study on ketone body metabolism in anaplastic thyroid cancer
Article Snippet: .. The samples were then transferred to the polyvinylidene fluoride (PVDF) membrane activated by methanol at 80 V for 90 min. Next, 5% skim milk was used to seal the PVDF membranes at room temperature for 2 h. Monoclonal antibodies p62 (HA721171, Huabio, China), Beclin1 (11306-1-AP, Proteintech, China), and LC3 (A19665, ABclonal, China) were then added, incubated overnight, and washed with TBST three times for 5 min each time. .. The samples were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit/mouse IgG (Beyotime, China) at room temperature for 1 h. Protein bands were detected using the enhanced chemiluminescence method, scanned, and analyzed using ImageJ software (National Institutes of Health, USA).

Ubiquitin Proteomics:

Article Title: USP38 regulates autophagy-dependent ferroptosis by deubiquitinating CTNNB1 in melanoma.
Article Snippet: The incidence of melanoma continues to rise gradually, and its clinical management remains challenging.. Ubiquitin-specific protease 38 (USP38) regulates tumor progression in multiple cancers, but its mechanism in melanoma remains elusive.. We discovered USP38 overexpression in human melanoma tissues.

AST Assay:

Article Title: P7C3 alleviates hepatic fibrosis via targeting eIF4A1-mediated protein translation and autophagy in hepatic stellate cells.
Article Snippet: P7C3, an aminopropyl carbazole compound with established neuroprotective properties and therapeutic potential in neurodegenerative disorders, has demonstrated broad pharmacological activity across multiple pathologies.. However, the effect of P7C3 on hepatic fibrosis remains unexplored.. This research applied in vitro and in vivo systems to evaluate P7C3’s antifibrotic efficacy.

Membrane:

Article Title: Preliminary study on ketone body metabolism in anaplastic thyroid cancer
Article Snippet: .. The samples were then transferred to the polyvinylidene fluoride (PVDF) membrane activated by methanol at 80 V for 90 min. Next, 5% skim milk was used to seal the PVDF membranes at room temperature for 2 h. Monoclonal antibodies p62 (HA721171, Huabio, China), Beclin1 (11306-1-AP, Proteintech, China), and LC3 (A19665, ABclonal, China) were then added, incubated overnight, and washed with TBST three times for 5 min each time. .. The samples were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit/mouse IgG (Beyotime, China) at room temperature for 1 h. Protein bands were detected using the enhanced chemiluminescence method, scanned, and analyzed using ImageJ software (National Institutes of Health, USA).

Bioprocessing:

Article Title: Preliminary study on ketone body metabolism in anaplastic thyroid cancer
Article Snippet: .. The samples were then transferred to the polyvinylidene fluoride (PVDF) membrane activated by methanol at 80 V for 90 min. Next, 5% skim milk was used to seal the PVDF membranes at room temperature for 2 h. Monoclonal antibodies p62 (HA721171, Huabio, China), Beclin1 (11306-1-AP, Proteintech, China), and LC3 (A19665, ABclonal, China) were then added, incubated overnight, and washed with TBST three times for 5 min each time. .. The samples were then incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit/mouse IgG (Beyotime, China) at room temperature for 1 h. Protein bands were detected using the enhanced chemiluminescence method, scanned, and analyzed using ImageJ software (National Institutes of Health, USA).



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In vitro evaluation of foam cell lipid accumulation and lipophagy activation following OPN-HMCN@MLT treatment. ( A - C ) ORO and BODIPY staining images and corresponding quantification of ORO and BODIPY positive areas of RAW264.7 cells under different stimulations (n = 5, scale bar for ORO: 100 μm, scale bar for BODIPY: 20 μm). ( D ) Bio-TEM images of RAW264.7 cells post various treatments (n = 5, scale bars 1.0 μm). Green arrows indicate nanoparticles. ( E , F ) Morphometric analysis determined the mean number and area (μm 2 ) of LDs per cell section. ( G ) Confocal images depicting lipophagy flux in foam cells following different treatments (n = 5 biological replicates, scale bars: 10 μm). ( H - J ) The quantities of acidified autophagosomes (GFP-RFP+), neutral autophagosomes (GFP + RFP+), and LDs labeled with BODIPY were measured per cell for each condition. (K to N) Representative Western blot images and quantitative analysis of LC3, LAMP1, and <t>P62</t> expression in foam cells. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.
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Image Search Results


ALI therapeutic mechanism. A) Co-immunofluorescent staining images of cells incubated with Cy5-CPs or Cy5-CPs@SS31 by confocal staining microscope: DAPI (blue), Mito-tracker green (green), and Cy5-CPs or Cy5-CPs@SS31 (red). (Scale bar = 10 μm) B) MMP of treated cells by flow cytometry. C) Heatmap of DEGs between control group and CPs@SS31+NIR: genes with relatively high (red) and low (blue) expression levels. D) Volcano plot of DEGs: 542 upregulated and 499 downregulated genes between control group and CPs@SS31+NIR. E) Anti-inflammation pathways related DEGs by KEGG enrichment analysis (red). F) Anti-inflammation pathways related differential biological functions by GO enrichment analysis (red): molecular function (MF), biological process (BP) and cell component (CC). G) Protein-protein interaction network of mitophagy related proteins. H) P62, Parkin and PINK1 expression levels of treated cells by WB. I) P62, Parkin and PINK1 expression levels in the lung tissue of treated rats. (Scale bar = 100 μm).

Journal: Bioactive Materials

Article Title: Near infrared enhanced palladium loaded siraitia grosvenorii carbon dots amplify mitophagy for acute lung injury immunotherapy

doi: 10.1016/j.bioactmat.2026.02.040

Figure Lengend Snippet: ALI therapeutic mechanism. A) Co-immunofluorescent staining images of cells incubated with Cy5-CPs or Cy5-CPs@SS31 by confocal staining microscope: DAPI (blue), Mito-tracker green (green), and Cy5-CPs or Cy5-CPs@SS31 (red). (Scale bar = 10 μm) B) MMP of treated cells by flow cytometry. C) Heatmap of DEGs between control group and CPs@SS31+NIR: genes with relatively high (red) and low (blue) expression levels. D) Volcano plot of DEGs: 542 upregulated and 499 downregulated genes between control group and CPs@SS31+NIR. E) Anti-inflammation pathways related DEGs by KEGG enrichment analysis (red). F) Anti-inflammation pathways related differential biological functions by GO enrichment analysis (red): molecular function (MF), biological process (BP) and cell component (CC). G) Protein-protein interaction network of mitophagy related proteins. H) P62, Parkin and PINK1 expression levels of treated cells by WB. I) P62, Parkin and PINK1 expression levels in the lung tissue of treated rats. (Scale bar = 100 μm).

Article Snippet: Furthermore, the membranes were separately incubated with the primary antibody (anti-P62, Parkin, PINK1 and GAPDH, Proteintech, China) overnight at 4 °C.

Techniques: Staining, Incubation, Microscopy, Flow Cytometry, Control, Expressing

In vitro evaluation of foam cell lipid accumulation and lipophagy activation following OPN-HMCN@MLT treatment. ( A - C ) ORO and BODIPY staining images and corresponding quantification of ORO and BODIPY positive areas of RAW264.7 cells under different stimulations (n = 5, scale bar for ORO: 100 μm, scale bar for BODIPY: 20 μm). ( D ) Bio-TEM images of RAW264.7 cells post various treatments (n = 5, scale bars 1.0 μm). Green arrows indicate nanoparticles. ( E , F ) Morphometric analysis determined the mean number and area (μm 2 ) of LDs per cell section. ( G ) Confocal images depicting lipophagy flux in foam cells following different treatments (n = 5 biological replicates, scale bars: 10 μm). ( H - J ) The quantities of acidified autophagosomes (GFP-RFP+), neutral autophagosomes (GFP + RFP+), and LDs labeled with BODIPY were measured per cell for each condition. (K to N) Representative Western blot images and quantitative analysis of LC3, LAMP1, and P62 expression in foam cells. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.

Journal: Bioactive Materials

Article Title: A foam cell-targeted lipophagy restoration strategy stabilizes vulnerable atherosclerotic plaques

doi: 10.1016/j.bioactmat.2026.02.041

Figure Lengend Snippet: In vitro evaluation of foam cell lipid accumulation and lipophagy activation following OPN-HMCN@MLT treatment. ( A - C ) ORO and BODIPY staining images and corresponding quantification of ORO and BODIPY positive areas of RAW264.7 cells under different stimulations (n = 5, scale bar for ORO: 100 μm, scale bar for BODIPY: 20 μm). ( D ) Bio-TEM images of RAW264.7 cells post various treatments (n = 5, scale bars 1.0 μm). Green arrows indicate nanoparticles. ( E , F ) Morphometric analysis determined the mean number and area (μm 2 ) of LDs per cell section. ( G ) Confocal images depicting lipophagy flux in foam cells following different treatments (n = 5 biological replicates, scale bars: 10 μm). ( H - J ) The quantities of acidified autophagosomes (GFP-RFP+), neutral autophagosomes (GFP + RFP+), and LDs labeled with BODIPY were measured per cell for each condition. (K to N) Representative Western blot images and quantitative analysis of LC3, LAMP1, and P62 expression in foam cells. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗ P < 0.001, and ∗∗∗∗ P < 0.0001.

Article Snippet: To block nonspecific binding, membranes were incubated with 5% skim milk for 1 h. Thereafter, membranes were incubated overnight at 4 °C with primary antibodies against ABCA1, ABCG1, ACOX1, CPT1A, LC3 (ab192890, 1:2000, abcam), LAMP1 (84658-5-RR, 1:8000, Proteintech), PPARα (66826-1-Ig, 1:3000, Proteintech), PPARγ (66936-1-Ig, 1:10000, Proteintech), P62 (18420-1-AP, 1:10000, Proteintech), MCAD (55210-1-AP, 1:3000, Proteintech), LCAD (17526-1-AP, 1:10000, Proteintech), tubulin (80762-1-RR, 1:10000, Proteintech), GAPDH (60004-1-Ig, 1:50000, Proteintech), and β-actin (66009-1-Ig, 1:20000, Proteintech).

Techniques: In Vitro, Activation Assay, Staining, Labeling, Western Blot, Expressing