p62 (Huabio Inc)
Structured Review
P62, supplied by Huabio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/p62/anti+p62/pm40407305-80-40-43
Average 86 stars, based on 1 article reviews
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Blocking Assay:Article Title: Traditional Chinese Medicinal Leech Induces Apoptosis and Autophagy in Glioblastoma by SGK1/Caspase-3 and PI3K/AKT/mTOR Pathway. Article Snippet: .. Following the blocking step, the membranes were maintained overnight at 4°C with the following antibodies: β- Actin (CST, #3700, 1:1000), GAPDH (Servicebio, GB11002, 1:1000), LC3B (Proteintech, 14,600- 1- AP, 1:1000), ATG5 (Huabio, ET611- 38, 1:1000), Western Blot:Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy Article Snippet: .. The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), Immunofluorescence:Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy Article Snippet: .. The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), Immunohistochemistry:Article Title: Enhanced oral nanomedicine utilizing biomineralized oncolytic virus for synergistic gastrointestinal cancer therapy Article Snippet: .. The antibodies used in western blot, immunofluorescence and immunohistochemistry experiments were as follows: CRT (Bioss, 5913R), HMGB1 (Beytime, AF1174), Anti-Adenovirus Type 5 Hexon (abcam, ab816352), Ki67 (Proteintech, 27309-1-AP), p53 (MCE, HY- P80938 ), p-p53 (Affinity, AF3075), p21 (Affinity, AF6290), CyclinB1 (Proteintech, 28603-1-AP), Bcl2 (Proteintech, 26593-1-AP), Bax (Proteintech, 50599-2-Ig), Caspase3 (Proteintech, 19677-1-AP), cleaved-Caspase3 (CST, 9661S), Cytochrome c (Proteintech, 10993-1-AP), other:Article Title: Integrating network pharmacology and experimental validation to elucidate the mechanism by which 2-Dihydroailanthone induces HCT116 cell death through suppressing autophagy via the AMPK/mTOR pathway. Article Snippet: Primary antibodies included Incubation:Article Title: USP38 regulates autophagy-dependent ferroptosis by deubiquitinating CTNNB1 in melanoma. Article Snippet: The incidence of melanoma continues to rise gradually, and its clinical management remains challenging.. Ubiquitin-specific protease 38 (USP38) regulates tumor progression in multiple cancers, but its mechanism in melanoma remains elusive.. We discovered USP38 overexpression in human melanoma tissues. Article Title: Preliminary study on ketone body metabolism in anaplastic thyroid cancer Article Snippet: .. The samples were then transferred to the polyvinylidene fluoride (PVDF) membrane activated by methanol at 80 V for 90 min. Next, 5% skim milk was used to seal the PVDF membranes at room temperature for 2 h. Ubiquitin Proteomics:Article Title: USP38 regulates autophagy-dependent ferroptosis by deubiquitinating CTNNB1 in melanoma. Article Snippet: The incidence of melanoma continues to rise gradually, and its clinical management remains challenging.. Ubiquitin-specific protease 38 (USP38) regulates tumor progression in multiple cancers, but its mechanism in melanoma remains elusive.. We discovered USP38 overexpression in human melanoma tissues. AST Assay:Article Title: P7C3 alleviates hepatic fibrosis via targeting eIF4A1-mediated protein translation and autophagy in hepatic stellate cells. Article Snippet: P7C3, an aminopropyl carbazole compound with established neuroprotective properties and therapeutic potential in neurodegenerative disorders, has demonstrated broad pharmacological activity across multiple pathologies.. However, the effect of P7C3 on hepatic fibrosis remains unexplored.. This research applied in vitro and in vivo systems to evaluate P7C3’s antifibrotic efficacy. Membrane:Article Title: Preliminary study on ketone body metabolism in anaplastic thyroid cancer Article Snippet: .. The samples were then transferred to the polyvinylidene fluoride (PVDF) membrane activated by methanol at 80 V for 90 min. Next, 5% skim milk was used to seal the PVDF membranes at room temperature for 2 h. Bioprocessing:Article Title: Preliminary study on ketone body metabolism in anaplastic thyroid cancer Article Snippet: .. The samples were then transferred to the polyvinylidene fluoride (PVDF) membrane activated by methanol at 80 V for 90 min. Next, 5% skim milk was used to seal the PVDF membranes at room temperature for 2 h. |

