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human bmp 2 cdna orf  (OriGene)


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    Structured Review

    OriGene human bmp 2 cdna orf
    Analysis of <t>BMP-2</t> variants in patients with TOLF. (a) Sequences showing two novel variants in BMP-2 (c.C460G:p.R154G, c.G584T:p.R195M), as compared with wild-type sequences. (b and c) Clustal W alignments showing conservation of mutated nucleotide (b) and amino acid (c) sequences.
    Human Bmp 2 Cdna Orf, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/origin+reference+frame+function/pmc05437907-137-5-20?v=OriGene
    Average 92 stars, based on 8 article reviews
    human bmp 2 cdna orf - by Bioz Stars, 2026-08
    92/100 stars

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    1) Product Images from "Two novel BMP-2 variants identified in patients with thoracic ossification of the ligamentum flavum"

    Article Title: Two novel BMP-2 variants identified in patients with thoracic ossification of the ligamentum flavum

    Journal: European Journal of Human Genetics

    doi: 10.1038/ejhg.2017.2

    Analysis of BMP-2 variants in patients with TOLF. (a) Sequences showing two novel variants in BMP-2 (c.C460G:p.R154G, c.G584T:p.R195M), as compared with wild-type sequences. (b and c) Clustal W alignments showing conservation of mutated nucleotide (b) and amino acid (c) sequences.
    Figure Legend Snippet: Analysis of BMP-2 variants in patients with TOLF. (a) Sequences showing two novel variants in BMP-2 (c.C460G:p.R154G, c.G584T:p.R195M), as compared with wild-type sequences. (b and c) Clustal W alignments showing conservation of mutated nucleotide (b) and amino acid (c) sequences.

    Techniques Used:

    Functional assays of wide-type and mutant BMP-2 transfected into MC3T3-E1 cells. (a) Exogenous BMP-2 expression as detected using the DDK-tag in different transfected cell lines. (b and c) Expression of osteogenic markers in different transfected cell lines at defined time points: ALP at day 7 (b); OCN and OPN at day 14 (c). (d) Staining for ALP activity at day 7 (upper) and alizarin red staining at day 14 (lower) in different transfected cell lines (scale bars represent 200 μm).
    Figure Legend Snippet: Functional assays of wide-type and mutant BMP-2 transfected into MC3T3-E1 cells. (a) Exogenous BMP-2 expression as detected using the DDK-tag in different transfected cell lines. (b and c) Expression of osteogenic markers in different transfected cell lines at defined time points: ALP at day 7 (b); OCN and OPN at day 14 (c). (d) Staining for ALP activity at day 7 (upper) and alizarin red staining at day 14 (lower) in different transfected cell lines (scale bars represent 200 μm).

    Techniques Used: Functional Assay, Mutagenesis, Transfection, Expressing, Staining, Activity Assay



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