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human orf for phf21b nm 138415 gfp  (OriGene)


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    OriGene human orf for phf21b nm 138415 gfp
    Figure 1 e Experimental assays for characterization of <t>PHF21B.</t> (A) Scheme of the PHF21B gene and its promoter-associated CpG islands organized according to the physical location on 22q13.31. This gene contains 13 exons spanning approximately 128 Kb of DNA (UCSC Genome Browser on Human, assembly GRCh37/hg19, http://genome.ucsc.edu) and a highly dense promoter-associated CpG island. This island spans 3336 bp, including the 50 region, with the first two exons of this gene overlapping three alternative transcription start sites: (*) transcript variant 1, NM_138415.4, (**) transcript variant 2, NM_001135862.2, and (***) transcript variant 3, NM_001242450.1. The comprehensive analysis of the PHF21B gene in HNSCC patients included several target regions for PCR-based assays for detection of the DNA copy number (30UTR), gene expression (exons 5e7), and mutations (exons 3, 6, 7, 8, 9, and 11). Changes in DNA methylation of CpG Island 273 were screened for three regions in HNSCC-derived cell lines. (B) DNA copy number changes in the PHF21B gene in matched normal (peripheral blood) and tumoral samples obtained from HNSCC patients. Constitutive losses of PHF21B were detected in cases showing a positive family cancer history (case 04), in case 50 (negative for family cancer history), and in cases 35, 49, and 71 (unknown information). Tumor-specific acquired losses were detected in
    Human Orf For Phf21b Nm 138415 Gfp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/origin+reference+frame+function/pm25454821-155-6-15?v=OriGene
    Average 90 stars, based on 1 article reviews
    human orf for phf21b nm 138415 gfp - by Bioz Stars, 2026-08
    90/100 stars

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    1) Product Images from "PHF21B as a candidate tumor suppressor gene in head and neck squamous cell carcinomas."

    Article Title: PHF21B as a candidate tumor suppressor gene in head and neck squamous cell carcinomas.

    Journal: Molecular oncology

    doi: 10.1016/j.molonc.2014.09.009

    Figure 1 e Experimental assays for characterization of PHF21B. (A) Scheme of the PHF21B gene and its promoter-associated CpG islands organized according to the physical location on 22q13.31. This gene contains 13 exons spanning approximately 128 Kb of DNA (UCSC Genome Browser on Human, assembly GRCh37/hg19, http://genome.ucsc.edu) and a highly dense promoter-associated CpG island. This island spans 3336 bp, including the 50 region, with the first two exons of this gene overlapping three alternative transcription start sites: (*) transcript variant 1, NM_138415.4, (**) transcript variant 2, NM_001135862.2, and (***) transcript variant 3, NM_001242450.1. The comprehensive analysis of the PHF21B gene in HNSCC patients included several target regions for PCR-based assays for detection of the DNA copy number (30UTR), gene expression (exons 5e7), and mutations (exons 3, 6, 7, 8, 9, and 11). Changes in DNA methylation of CpG Island 273 were screened for three regions in HNSCC-derived cell lines. (B) DNA copy number changes in the PHF21B gene in matched normal (peripheral blood) and tumoral samples obtained from HNSCC patients. Constitutive losses of PHF21B were detected in cases showing a positive family cancer history (case 04), in case 50 (negative for family cancer history), and in cases 35, 49, and 71 (unknown information). Tumor-specific acquired losses were detected in
    Figure Legend Snippet: Figure 1 e Experimental assays for characterization of PHF21B. (A) Scheme of the PHF21B gene and its promoter-associated CpG islands organized according to the physical location on 22q13.31. This gene contains 13 exons spanning approximately 128 Kb of DNA (UCSC Genome Browser on Human, assembly GRCh37/hg19, http://genome.ucsc.edu) and a highly dense promoter-associated CpG island. This island spans 3336 bp, including the 50 region, with the first two exons of this gene overlapping three alternative transcription start sites: (*) transcript variant 1, NM_138415.4, (**) transcript variant 2, NM_001135862.2, and (***) transcript variant 3, NM_001242450.1. The comprehensive analysis of the PHF21B gene in HNSCC patients included several target regions for PCR-based assays for detection of the DNA copy number (30UTR), gene expression (exons 5e7), and mutations (exons 3, 6, 7, 8, 9, and 11). Changes in DNA methylation of CpG Island 273 were screened for three regions in HNSCC-derived cell lines. (B) DNA copy number changes in the PHF21B gene in matched normal (peripheral blood) and tumoral samples obtained from HNSCC patients. Constitutive losses of PHF21B were detected in cases showing a positive family cancer history (case 04), in case 50 (negative for family cancer history), and in cases 35, 49, and 71 (unknown information). Tumor-specific acquired losses were detected in

    Techniques Used: Variant Assay, Gene Expression, DNA Methylation Assay, Derivative Assay

    Figure 2 e Clonogenesis and migration assays performed for PHF21B. (A) Amino acid sequence alignment between PHF21A and PHF21B reveals conservation of domains important for transcriptional repression. The sequences from the two proteins were aligned using BLASTP. Important functional domains from PHF21A were visualized by UNIPROT: a GLN-rich region is found between residues 4-108; a DNA binding domain A.T Hook is found between 425 and 437; a PHD-type zinc finger domain is found between 488 and 535; a region required for transcriptional repression is found between 486 and 680. (BeH) Subcellular distribution of endogenous and transfected PHF21B. FaDU (B and C) and SCC4 (D)
    Figure Legend Snippet: Figure 2 e Clonogenesis and migration assays performed for PHF21B. (A) Amino acid sequence alignment between PHF21A and PHF21B reveals conservation of domains important for transcriptional repression. The sequences from the two proteins were aligned using BLASTP. Important functional domains from PHF21A were visualized by UNIPROT: a GLN-rich region is found between residues 4-108; a DNA binding domain A.T Hook is found between 425 and 437; a PHD-type zinc finger domain is found between 488 and 535; a region required for transcriptional repression is found between 486 and 680. (BeH) Subcellular distribution of endogenous and transfected PHF21B. FaDU (B and C) and SCC4 (D)

    Techniques Used: Migration, Sequencing, Functional Assay, Binding Assay, Transfection



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