chip dna binding buffer (Zymo Research)
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Chip Dna Binding Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligonucleotide+microarray+experiments/ChIP+DNA+Binding+Buffer/pmc07052505-67-5-4
Average 93 stars, based on 17 article reviews
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Incubation:Article Title: Enhanced oligonucleotides for inhibiting RTEL1 expression Article Snippet: Samples were thawed at 37° C. and 1 μl of Proteinase K (cat no. AM25448 @ Ambion biosciences, 20 mg/mL Stock) was added to each well of the 96-well plate and samples were incubated at 56° C. for 30 minutes. .. After incubation, 3 volumes of Chromatin Immunoprecipitation:Article Title: Enhanced oligonucleotides for inhibiting RTEL1 expression Article Snippet: Samples were thawed at 37° C. and 1 μl of Proteinase K (cat no. AM25448 @ Ambion biosciences, 20 mg/mL Stock) was added to each well of the 96-well plate and samples were incubated at 56° C. for 30 minutes. .. After incubation, 3 volumes of Article Title: Precise modulation of transcription factor levels identifies features underlying dosage sensitivity Article Snippet: .. ChIP and input samples were purified by Zymo DNA Clean & Concentrator Kit with Article Title: High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS) Article Snippet: .. Article Title: High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS) Article Snippet: Each sample was then digested with 10 mg/ml RNase A (Thermo Fisher Scientific, MA, Cat# EN0531) at 42°C for 2 hr. .. Genomic DNA was then reisolated by adding a 2:1 ratio of Article Title: Restriction Endonuclease-Based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing. Article Snippet: For the experiment in Fig. 3B, the same protocol was followed as in Fig. 3A except after the EcoRII incubation, 8 mL of NEB purple loading dye was added and the samples were loaded into a 1% agarose gel and run for 45 min at 120 V. The high-molecular-weight bands were excised with a razor and dissolved in 3 volumes of Zymo agarose dissolving buffer (Zymo D4001) at 50°C. .. They were then processed January 2023 Volume 89 Issue 1 10.1128/aem.01670-22 12 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a em o n 19 J ul y 20 23 b y 2a 0d :6 fc 0: 89 b: ae 00 :e d1 c: 28 2f :f 10 0: a6 72 . through the Zymo genomic clean and concentrate kit as in Fig. 3A excluding the step of the addition of Binding Assay:Article Title: Enhanced oligonucleotides for inhibiting RTEL1 expression Article Snippet: Samples were thawed at 37° C. and 1 μl of Proteinase K (cat no. AM25448 @ Ambion biosciences, 20 mg/mL Stock) was added to each well of the 96-well plate and samples were incubated at 56° C. for 30 minutes. .. After incubation, 3 volumes of Article Title: High-Throughput Library Transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS) Article Snippet: Each sample was then digested with 10mg/ml RNase A (ThermoFisher Scientific Massachussets, USA, Cat. No. EN0531) at 42C° for 2 hours. .. Genomic DNA was then reisolated by adding a 2:1 ratio of Article Title: High-Throughput Library Transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS) Article Snippet: .. Article Title: Precise modulation of transcription factor levels identifies features underlying dosage sensitivity Article Snippet: .. ChIP and input samples were purified by Zymo DNA Clean & Concentrator Kit with Article Title: High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS) Article Snippet: .. Article Title: High-throughput library transgenesis in Caenorhabditis elegans via Transgenic Arrays Resulting in Diversity of Integrated Sequences (TARDIS) Article Snippet: Each sample was then digested with 10 mg/ml RNase A (Thermo Fisher Scientific, MA, Cat# EN0531) at 42°C for 2 hr. .. Genomic DNA was then reisolated by adding a 2:1 ratio of Article Title: Restriction Endonuclease-Based Modification-Dependent Enrichment (REMoDE) of DNA for Metagenomic Sequencing. Article Snippet: For the experiment in Fig. 3B, the same protocol was followed as in Fig. 3A except after the EcoRII incubation, 8 mL of NEB purple loading dye was added and the samples were loaded into a 1% agarose gel and run for 45 min at 120 V. The high-molecular-weight bands were excised with a razor and dissolved in 3 volumes of Zymo agarose dissolving buffer (Zymo D4001) at 50°C. .. They were then processed January 2023 Volume 89 Issue 1 10.1128/aem.01670-22 12 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/a em o n 19 J ul y 20 23 b y 2a 0d :6 fc 0: 89 b: ae 00 :e d1 c: 28 2f :f 10 0: a6 72 . through the Zymo genomic clean and concentrate kit as in Fig. 3A excluding the step of the addition of Purification:Article Title: Enhanced oligonucleotides for inhibiting RTEL1 expression Article Snippet: Samples were thawed at 37° C. and 1 μl of Proteinase K (cat no. AM25448 @ Ambion biosciences, 20 mg/mL Stock) was added to each well of the 96-well plate and samples were incubated at 56° C. for 30 minutes. .. After incubation, 3 volumes of Article Title: Precise modulation of transcription factor levels identifies features underlying dosage sensitivity Article Snippet: .. ChIP and input samples were purified by Zymo DNA Clean & Concentrator Kit with |

