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Incyte corporation gem mouse cdna array
Gem Mouse Cdna Array, supplied by Incyte corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/oligonucleotide+microarray+experiments/mouse+gem1+array/pm15525354-52-3-7
Average 90 stars, based on 1 article reviews
gem mouse cdna array - by Bioz Stars, 2026-09
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Article Title: Divergent roles of lysyl oxidase family members in ornithine decarboxylase- and RAS-transformed mouse fibroblasts and human melanoma cells
Article Snippet: Polyadenylated RNAs were extracted from the cells and analyzed with Mouse GEM2/Unigene1 cDNA LifeArrays (Incyte Genomics, Palo Alto, CA, USA), as previously described [ , ].

Article Title: Changes in gene expression during the development of mammary tumors in MMTV- Wnt-1 transgenic mice
Article Snippet: The 8.7k slides contain the 8700 Incyte GEM1 clone set, which are mapped to 6,877 Unigene cluster IDs, among which 2,953 are named genes, 2,206 are expressed sequence tags, and 1,628 are Riken cDNAs.

Article Title: Changes in gene expression during the development of mammary tumors in MMTV- Wnt-1 transgenic mice
Article Snippet: The 15k slides contain the 8700 Incyte GEM1 clone set and the mammary 6000 clone set; a total of 1,444 clones do not map to a Unigene cluster ID, whereas the rest of the clones map to 10,062 unique genes as defined by Unigene cluster ID.

Article Title: Chromosome-wide identification of novel imprinted genes using microarrays and uniparental disomies
Article Snippet: , Incyte™ GEM 1 , , x , , , x , x , .

Clone Assay:

Article Title: Domain-oriented functional analysis based on expression profiling
Article Snippet: .. In this study, each member of Pfam families was mapped to Incyte clones which were presented in the Incyte microarray chips based on the sequence identity. .. We then generated mRNA expression profiles for these Pfam family members using Incyte's LifeExpress RNA (LE) database (Version 3.0, April 2001 release, Incyte Genomics, Inc.).

Microarray:

Article Title: Domain-oriented functional analysis based on expression profiling
Article Snippet: .. In this study, each member of Pfam families was mapped to Incyte clones which were presented in the Incyte microarray chips based on the sequence identity. .. We then generated mRNA expression profiles for these Pfam family members using Incyte's LifeExpress RNA (LE) database (Version 3.0, April 2001 release, Incyte Genomics, Inc.).

Article Title: Microarray and comparative genomics-based identification of genes and gene regulatory regions of the mouse immune system
Article Snippet: .. To do this, we used the Incyte Mouse GEM1 microarray, an 8638 element spotted cDNA gene expression platform and a universal reference design that employed poly A+ mRNA was prepared from whole day-1 postnatal mouse. ..

Sequencing:

Article Title: Domain-oriented functional analysis based on expression profiling
Article Snippet: .. In this study, each member of Pfam families was mapped to Incyte clones which were presented in the Incyte microarray chips based on the sequence identity. .. We then generated mRNA expression profiles for these Pfam family members using Incyte's LifeExpress RNA (LE) database (Version 3.0, April 2001 release, Incyte Genomics, Inc.).

BAC Assay:

Article Title: Defining the functionally sufficient regulatory region and liver-specific roles of the erythropoietin gene by transgene complementation.
Article Snippet: Background: Erythropoietin (EPO) is an essential growth factor for erythroid cells and is mainly secreted from the kidneys and subsidiarily from the livers of adult mammals in an anemia/hypoxia-inducible manner.. Aim and method: To elucidate the regulatory mechanisms of stress-inducible and cell type-specific Epo gene transcription, the rate-limiting step of EPO production, we investigated the sufficiency of a 180-kb genomic fragment flanking the mouse Epo gene locus for recapitulating endogenous Epo gene function by a transgene complementation strategy.. Key findings: While Epo gene-deficient mice exhibited lethal anemia in utero with defects in erythroblast proliferation and maturation, Epo-knockout mice integrated with the 180-kb Epo transgene showed normal erythropoiesis throughout life.

Homologous Recombination:

Article Title: Defining the functionally sufficient regulatory region and liver-specific roles of the erythropoietin gene by transgene complementation.
Article Snippet: Background: Erythropoietin (EPO) is an essential growth factor for erythroid cells and is mainly secreted from the kidneys and subsidiarily from the livers of adult mammals in an anemia/hypoxia-inducible manner.. Aim and method: To elucidate the regulatory mechanisms of stress-inducible and cell type-specific Epo gene transcription, the rate-limiting step of EPO production, we investigated the sufficiency of a 180-kb genomic fragment flanking the mouse Epo gene locus for recapitulating endogenous Epo gene function by a transgene complementation strategy.. Key findings: While Epo gene-deficient mice exhibited lethal anemia in utero with defects in erythroblast proliferation and maturation, Epo-knockout mice integrated with the 180-kb Epo transgene showed normal erythropoiesis throughout life.

Bacteria:

Article Title: Defining the functionally sufficient regulatory region and liver-specific roles of the erythropoietin gene by transgene complementation.
Article Snippet: Background: Erythropoietin (EPO) is an essential growth factor for erythroid cells and is mainly secreted from the kidneys and subsidiarily from the livers of adult mammals in an anemia/hypoxia-inducible manner.. Aim and method: To elucidate the regulatory mechanisms of stress-inducible and cell type-specific Epo gene transcription, the rate-limiting step of EPO production, we investigated the sufficiency of a 180-kb genomic fragment flanking the mouse Epo gene locus for recapitulating endogenous Epo gene function by a transgene complementation strategy.. Key findings: While Epo gene-deficient mice exhibited lethal anemia in utero with defects in erythroblast proliferation and maturation, Epo-knockout mice integrated with the 180-kb Epo transgene showed normal erythropoiesis throughout life.

Gene Expression:

Article Title: Microarray and comparative genomics-based identification of genes and gene regulatory regions of the mouse immune system
Article Snippet: .. To do this, we used the Incyte Mouse GEM1 microarray, an 8638 element spotted cDNA gene expression platform and a universal reference design that employed poly A+ mRNA was prepared from whole day-1 postnatal mouse. ..

Purification:

Article Title: At Least Ten Genes Define the Imprinted Dlk1-Dio3 Cluster on Mouse Chromosome 12qF1
Article Snippet: .. Poly A+ RNA purified from both AG and PG PMEFs were subjected to RNA expression profiling using an Incyte mouse GEM1 array, containing 8,638 cDNA probes corresponding to identified genes . ..

RNA Expression:

Article Title: At Least Ten Genes Define the Imprinted Dlk1-Dio3 Cluster on Mouse Chromosome 12qF1
Article Snippet: .. Poly A+ RNA purified from both AG and PG PMEFs were subjected to RNA expression profiling using an Incyte mouse GEM1 array, containing 8,638 cDNA probes corresponding to identified genes . ..



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A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from <t>microarray</t> analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .
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Comparison of transcript quantification by Affymetrix <t>microarray</t> with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.
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A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

Journal: Journal of Alzheimer's Disease Reports

Article Title: Transcriptomic Analysis of Alzheimer’s Disease Pathways in a Pakistani Population 1

doi: 10.3233/ADR-230146

Figure Lengend Snippet: A) The stacked bar chart represents a summary of total upregulated (red) and downregulated (green) genes representing 22 important signaling and disease pathways in AD subjects compared to controls. The output core analysis, reflecting the differential gene expressions obtained from the microarrays (gene sets with≥2-fold change, t -test, p < 0.05). B) Ingenuity Pathway Analysis (IPA)-derived Amyloid Processing network of differentially expressed genes derived from microarray analysis. IPA analysis identified a group of genes expression status and their potential interactive links in the context of Amyloid Processing, Neuronal Death. We noted activation of Gamma Secretase, Beta Secretase, upregulation of ERK1/2 CK1/2 P38MAPK, PKA, PRKCE, CDK5 , and CDK5R1 and downregulation of MAPT , and GSK3B .

Article Snippet: The oligonucleotide microarray experiments were conducted by EpigenDx (Boston, MA) using the Affymetrix U133 Plus 2.0 Array platform, which has comprehensive coverage of the whole transcribed human genome on a single array.

Techniques: Derivative Assay, Microarray, Expressing, Activation Assay

Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Quantitative Protein and mRNA Profiling Shows Selective Post-Transcriptional Control of Protein Expression by Vasopressin in Kidney Cells *

doi: 10.1074/mcp.M110.004036

Figure Lengend Snippet: Comparison of transcript quantification by Affymetrix microarray with real time RT-PCR. A 200 ng aliquot total RNA from dDAVP- and vehicle-exposed mpkCCD cells was used for quantification of transcript abundances with real time RT-PCR. *, statistically significant versus no change i.e. log2(dDAVP/vehicle) = 0 (p < 0.05, n = 3). The RefSeq accession numbers are: Akap12, NM_031185; Aqp2, NM_009699; Asap2, NM_001004364; C3, NM_009778; Clmn, NM_001040682; Cpt1a, NM_013495; Fth1, NM_010239; Gsdmc1, NM_031378; Gsdmc2, NM_177912; Gsdmc4, XM_001474104; Gstt3, NM_133994; Idh1, NM_001111320; Mon2, NM_153395; Osbpl1a, NM_207530; Spnb3, NM_021287; and Trip11, XM_001001171.

Article Snippet: To address whether changes in protein abundance measured by SILAC LC-MS/MS are generally because of corresponding changes in mRNA levels, we carried out oligonucleotide microarray experiments (Affymetrix) in mpkCCD cells treated in the same manner (0.1 n m dDAVP or vehicle for 5 days).

Techniques: Microarray, Quantitative RT-PCR