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custom oligonucleotide microarrays  (NimbleGen Systems GmbH)

 
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    NimbleGen Systems GmbH custom oligonucleotide microarrays
    Abundance of transcripts of KD1 downstream genes. Total RNA isolated from pedicel AZs of wild-type (cv New Yorker [NY]) and TAPG4::antisense KD1 transgenic (line E) plants 4 h after flower removal (A), and freshly harvested wild-type (cv VF36) and Pts mutant (B) plants were used to determine abundance of transcripts of each gene by qRT-PCR. Abundance of tomato 26S rRNA was used as an internal control. The expression ratio of each gene from <t>microarray</t> analysis is shown above the corresponding qRT-PCR column. Different letters indicate significant differences between NY and line E or between cv VF36 and Pts at each time point (Student’s t test, P < 0.05). Results are the means of three biological replicates ± sd. *, Data representing PIN9 were absent in the microarray.
    Custom Oligonucleotide Microarrays, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/oligonucleotide+microarray+analyses/custom+oligonucleotide+array/pmc04348773-351-0-7
    Average 90 stars, based on 1 article reviews
    custom oligonucleotide microarrays - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "A KNOTTED1-LIKE HOMEOBOX Protein Regulates Abscission in Tomato by Modulating the Auxin Pathway 1 [OPEN] "

    Article Title: A KNOTTED1-LIKE HOMEOBOX Protein Regulates Abscission in Tomato by Modulating the Auxin Pathway 1 [OPEN]

    Journal: Plant Physiology

    doi: 10.1104/pp.114.253815

    Abundance of transcripts of KD1 downstream genes. Total RNA isolated from pedicel AZs of wild-type (cv New Yorker [NY]) and TAPG4::antisense KD1 transgenic (line E) plants 4 h after flower removal (A), and freshly harvested wild-type (cv VF36) and Pts mutant (B) plants were used to determine abundance of transcripts of each gene by qRT-PCR. Abundance of tomato 26S rRNA was used as an internal control. The expression ratio of each gene from microarray analysis is shown above the corresponding qRT-PCR column. Different letters indicate significant differences between NY and line E or between cv VF36 and Pts at each time point (Student’s t test, P < 0.05). Results are the means of three biological replicates ± sd. *, Data representing PIN9 were absent in the microarray.
    Figure Legend Snippet: Abundance of transcripts of KD1 downstream genes. Total RNA isolated from pedicel AZs of wild-type (cv New Yorker [NY]) and TAPG4::antisense KD1 transgenic (line E) plants 4 h after flower removal (A), and freshly harvested wild-type (cv VF36) and Pts mutant (B) plants were used to determine abundance of transcripts of each gene by qRT-PCR. Abundance of tomato 26S rRNA was used as an internal control. The expression ratio of each gene from microarray analysis is shown above the corresponding qRT-PCR column. Different letters indicate significant differences between NY and line E or between cv VF36 and Pts at each time point (Student’s t test, P < 0.05). Results are the means of three biological replicates ± sd. *, Data representing PIN9 were absent in the microarray.

    Techniques Used: Isolation, Transgenic Assay, Mutagenesis, Quantitative RT-PCR, Control, Expressing, Microarray

    Related Articles

    other:

    Article Title: Method of obtaining antibodies of interest and nucleotides encoding same
    Article Snippet: A custom oligonucleotide array (NimbleGen Systems, Inc., Madison, Wis.) is placed over said fabricated microwells in order to capture and tag mRNAs.

    Article Title: Multi-well system
    Article Snippet: A custom oligonucleotide array (NimbleGen Systems, Inc., Madison, Wis.) is placed over said fabricated micro-wells in order to capture and tag mRNAs.

    Article Title: Method of obtaining antibodies of interest and nucleotides encoding same
    Article Snippet: A custom oligonucleotide array (NimbleGen Systems, Inc., Madison, Wis.) is placed over said commercial microwell array to capture and tag mRNAs.

    Article Title: Method of obtaining antibodies of interest and nucleotides encoding same
    Article Snippet: A custom oligonucleotide array (NimbleGen Systems, Inc., Madison, Wis.) is placed over said commercial microwell array to capture and tag mRNAs.

    Article Title: Method of obtaining antibodies of interest and nucleotides encoding same
    Article Snippet: A custom oligonucleotide array (NimbleGen Systems, Inc., Madison, Wis.) is placed over said PicoTiterPlate to capture and tag mRNAs.

    Microarray:

    Article Title: A KNOTTED1-LIKE HOMEOBOX Protein Regulates Abscission in Tomato by Modulating the Auxin Pathway
    Article Snippet: .. Microarray Custom oligonucleotide microarrays were fabricated by NimbleGen Systems, Inc. using photolithography directed by the Maskless Array Synthesizer ( Singh-Gasson et al., 1999 ). ..



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