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incucyte zoom phase only processing module  (Sartorius AG)


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    Structured Review

    Sartorius AG incucyte zoom phase only processing module
    SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the <t>Incucyte</t> automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm
    Incucyte Zoom Phase Only Processing Module, supplied by Sartorius AG, used in various techniques. Bioz Stars score: 99/100, based on 16124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ocr+module/Live+Cell+Analysis+Instruments/pmc07412812-498-7-12
    Average 99 stars, based on 16124 article reviews
    incucyte zoom phase only processing module - by Bioz Stars, 2026-11
    99/100 stars

    Images

    1) Product Images from "The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation"

    Article Title: The RNA-binding protein SERBP1 functions as a novel oncogenic factor in glioblastoma by bridging cancer metabolism and epigenetic regulation

    Journal: Genome Biology

    doi: 10.1186/s13059-020-02115-y

    SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the Incucyte automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm
    Figure Legend Snippet: SERBP1 affects cancer-related phenotypes and tumor growth. a Knockdown of SERBP1 in U251 cells decreased viability (MTS assay). b SERBP1 silencing diminished clonogenic potential, as measured by colony formation assays. c The Boyden chamber assay was used to evaluate SERBP1 impact on invasion; values of crystal violet absorbance showed that SERBP1 knockdown decreased invasion potential. d – f SERBP1 silencing increased apoptosis as indicated by PARP1 cleavage ( d ), annexin staining ( e ), and caspase ( f ). g GSC proliferation across time was followed with the Incucyte automated system. Decrease in SERBP1 levels impaired cell proliferation. i Knockdown of SERBP1 in GSC lines decreased viability (MTS assays). Data were analyzed with Student’s t test and presented as the mean ± standard deviation. Bonferroni correction was used for multiple comparisons. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001. i Intracranial xenografts were established using the shSERBP1 3565 GSC cell line (10 mice per group). Experimental group received Dox to induce expression of shSERBP1. Kaplan-Meier curves indicate that SERBP1 knockdown decreased tumor growth and expanded survival. j Representative Ki67 staining from the tumor boundary for each group. Scale bar = 100 μm

    Techniques Used: MTS Assay, Boyden Chamber Assay, Staining, Standard Deviation, Expressing

    Related Articles

    Cell Characterization:

    Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer
    Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the IncuCyte cell count proliferation assay (Sartorius). ..

    Proliferation Assay:

    Article Title: Targeting YAP/TEAD signaling disturbs RNA Pol II activity and enhances immunotherapy response via activated cytosolic DNA sensing pathway in gastroesophageal cancer
    Article Snippet: Cells were cultured in RPMI1640 (Invitrogen) or DMEM (Invitrogen) and supplemented with 10% fetal bovine serum (FBS) (Invitrogen), 100 U/ml penicillin, and 100 mg/ml streptomycin (Invitrogen), and maintained at 37°C in a humidified incubator of 5% CO2. .. Live-Cell proliferation was measured using the IncuCyte cell count proliferation assay (Sartorius). ..

    Software:

    Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research
    Article Snippet: .. IncuCyte software analysis , Sartorius , RRID:SCR_003935; https://www.sartorius.com/en/product. ..

    Article Title: Targeting CD28 on T lineage malignancies with chimeric antigen receptor T cells.
    Article Snippet: .. Quantification of cellular abundance was performed as recommended by measuring GFP integrated intensity in at least technical triplicates (Sartorius, Incucyte Software Version 2023A-2025B). ..

    Article Title: Astrocytes regulate axonal mitochondrial transport deficits in C9ORF72 amyotrophic lateral sclerosis motor neurons.
    Article Snippet: .. Software and code Policy information about availability of computer code Data collection Zeiss Zen software (Carl Zeiss), Seahorse Wave Desktop Software v2.6 (Agilent Technologies), Harmony software v4.9 (PerkinElmer), IncuCyte software (Sartorius), and ImageJ/Fiji v2.14. .. Data analysis GraphPad Prism v10, R v4.3.2 (including lme4, car, emmeans and DESeq2 packages), and ImageJ/Fiji v2.14 For manuscripts utilizing custom algorithms or software that are central to the research but not yet described in published literature, software must be made available to editors and reviewers.

    Analysis:

    Article Title: Species-specific inflammatory responses of chondrocytes: Toward evidence-based model selection in OA research
    Article Snippet: .. IncuCyte software analysis , Sartorius , RRID:SCR_003935; https://www.sartorius.com/en/product. ..

    Article Title: Differential effects of propranolol enantiomers on haemangioma-derived endothelial spheroids
    Article Snippet: The spheroids were embedded in Cultrex Reduced Growth Factor Basement Membrane Extract (R&D Systems, NE Minneapolis, MN, USA) at a concentration of 4–5 mg/mL, following removal of growth medium and incubated at 37 °C to allow polymerisation. .. Culture media was added on top and whole spheroid area and the sprouting area was monitored over 3 days with the Incucyte Live-Cell Analysis (Sartorius, Essen Bio, Ann Arbor, MI, USA) with scans performed every 24 h using a 4 × objective. ..

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).

    Staining:

    Article Title: Gasdermin D-mediated delivery of caspase inhibitors to suppress pyroptosis.
    Article Snippet: Caspase-1, -4, -5 and -11 activate gasdermin D (GSDMD) pores, causing pyroptotic cell death and the release of the interleukins IL-1β and IL-18 (ref. 1).. Blocking this pathway holds therapeutic promise for the treatment of inflammatory disorders, but cellpermeable caspase inhibitors have not proved successful in clinical trials.. Here we describe covalent caspase inhibitors that selectively block pyroptosis and IL-1β secretion despite being excluded from healthy cells.

    Morphology:

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).

    Incubation:

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).

    Phase Contrast:

    Article Title: Cold-Atmospheric-Plasma-Treated MSCs Retain Stem Cell Phenotype and Trilineage Differentiation Potential
    Article Snippet: .. Changes in hTERT-MSC fibroblast-like morphology were investigated following each treatment for the duration of the incubation (24, 48, 72 h) using the Incucyte Live-Cell https://doi.org/10.3390/plasma9040043 Analysis Instrument (Sartorius, Göttingen, Germany) in phase-contrast mode with the S3/SX 1 G/R Optical Module. .. To determine the MSC phenotype of hTERT-MSCs, the expression of positive markers CD105, CD90 and CD73 and the negative marker HLA/DR was analysed by flow cytometry using an MSC Phenotyping Cocktail Kit, anti-human, REAfinity (Miltenyi Biotec, Bisley, UK).



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