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Image Search Results
Journal: Animals : an Open Access Journal from MDPI
Article Title: Familial Robertsonian Translocation Involving Two Autosomes in Swiss Entlebucher Mountain Dogs
doi: 10.3390/ani16203274
Figure Lengend Snippet: ( a , b ) show the same metaphase of bitch 1 (heterozygous for RT) before (4′,6-diamidino-2-phenylindole (DAPI) and after fluorescence in situ hybridization with the Robertsonian translocation painting probe, respectively. Comparatively, ( d , e ) show the results for bitch 4 (homozygous for Robertsonian translocation). ( c ) shows clear hybridization signals of the Robertsonian translocation painting probe in an interphase nucleus of bitch 1. Green color and red color are the epiluminescence of fluorescein-12-dUTP (channel green) and aminoallyl-dUTP-Texas-Red (channel red), respectively.
Article Snippet: After two rounds of degenerate oligo-primed polymerase chain reaction (PCR) using a hand-mixed Telenius primer (5’CCGACTCGAGNNNNNNATGTGG3’) [ ], amplified DNA was labeled with Fluorescein-12-dUTP or
Techniques: Fluorescence, In Situ Hybridization, Translocation Assay, Hybridization