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histological analysis tissue microarray  (OriGene)


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    Structured Review

    OriGene histological analysis tissue microarray
    (A) Immunoblot of STING in hTERT fibroblasts, normal human colon epithelial (FHC) and a series of human colon cancer cell lines. (B) ELISA analysis of human Interferon β production in the media of cells (same as A) transfected with 3μg/ml polyIC or dsDNA90 or mock transfected for 16 hours. (C) qPCR analysis of human CXCL10 expression in cells (same as A) transfected with 3μg/ml dsDNA90 or mock transfected for 3 hours. (D) qPCR analysis of human IL1B expression in cells same as C. Data is representative of at least two independent experiments. Error bars indicate s.d. *, p<0.05; **, p<0.01; ***, p<0.001; Student’s t-test. (E) <t>Microarray</t> analysis of gene expression in indicated normal and colon cancer cells mock transfected or transfected with 3μg/ml dsDNA90 for 3 hours. Highest variable genes are shown. Rows represent individual genes; columns represent individual samples. Pseudo-colors indicate transcript levels below (green), equal to (black), or above (red) the mean. Scale represents the intensity of gene expression (log10 scale ranges between −3 and 3). (F) Fold change values of highest variable genes shown in E. See also Figure S1 and S2.
    Histological Analysis Tissue Microarray, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/normal+tissue+microarrays/Normal+Tissue+Microarray/pmc04845097-467-2-9
    Average 91 stars, based on 9 article reviews
    histological analysis tissue microarray - by Bioz Stars, 2026-09
    91/100 stars

    Images

    1) Product Images from "Deregulation of STING Signaling in Colorectal Carcinoma Constrains DNA-Damage Responses and Correlates With Tumorigenesis"

    Article Title: Deregulation of STING Signaling in Colorectal Carcinoma Constrains DNA-Damage Responses and Correlates With Tumorigenesis

    Journal: Cell reports

    doi: 10.1016/j.celrep.2015.12.029

    (A) Immunoblot of STING in hTERT fibroblasts, normal human colon epithelial (FHC) and a series of human colon cancer cell lines. (B) ELISA analysis of human Interferon β production in the media of cells (same as A) transfected with 3μg/ml polyIC or dsDNA90 or mock transfected for 16 hours. (C) qPCR analysis of human CXCL10 expression in cells (same as A) transfected with 3μg/ml dsDNA90 or mock transfected for 3 hours. (D) qPCR analysis of human IL1B expression in cells same as C. Data is representative of at least two independent experiments. Error bars indicate s.d. *, p<0.05; **, p<0.01; ***, p<0.001; Student’s t-test. (E) Microarray analysis of gene expression in indicated normal and colon cancer cells mock transfected or transfected with 3μg/ml dsDNA90 for 3 hours. Highest variable genes are shown. Rows represent individual genes; columns represent individual samples. Pseudo-colors indicate transcript levels below (green), equal to (black), or above (red) the mean. Scale represents the intensity of gene expression (log10 scale ranges between −3 and 3). (F) Fold change values of highest variable genes shown in E. See also Figure S1 and S2.
    Figure Legend Snippet: (A) Immunoblot of STING in hTERT fibroblasts, normal human colon epithelial (FHC) and a series of human colon cancer cell lines. (B) ELISA analysis of human Interferon β production in the media of cells (same as A) transfected with 3μg/ml polyIC or dsDNA90 or mock transfected for 16 hours. (C) qPCR analysis of human CXCL10 expression in cells (same as A) transfected with 3μg/ml dsDNA90 or mock transfected for 3 hours. (D) qPCR analysis of human IL1B expression in cells same as C. Data is representative of at least two independent experiments. Error bars indicate s.d. *, p<0.05; **, p<0.01; ***, p<0.001; Student’s t-test. (E) Microarray analysis of gene expression in indicated normal and colon cancer cells mock transfected or transfected with 3μg/ml dsDNA90 for 3 hours. Highest variable genes are shown. Rows represent individual genes; columns represent individual samples. Pseudo-colors indicate transcript levels below (green), equal to (black), or above (red) the mean. Scale represents the intensity of gene expression (log10 scale ranges between −3 and 3). (F) Fold change values of highest variable genes shown in E. See also Figure S1 and S2.

    Techniques Used: Western Blot, Enzyme-linked Immunosorbent Assay, Transfection, Expressing, Microarray, Gene Expression

    Related Articles

    Microarray:

    Article Title: Cancer treatment and diagnosis
    Article Snippet: Tissue Microarray was purchased from Origene. .. Tissue Microarray was purchased from Origene. ..

    Article Title: BACH1 promotes the progression of esophageal squamous cell carcinoma by inducing the epithelial-mesenchymal transition and angiogenesis.
    Article Snippet: Tissue microarrays of ESCC, HEsoS105Su01 included 50 tumor tissues and paired esophageal epithelial tissues and ZH- ESC 77a included 77 tumor tissues, were purchased from Outdo Biotech and Zhuohao Medical Science and Technology (Shanghai, China). .. Slides of the tissue microarray were incubated with 1:200 diluted BACH1 antibody (#A5393, Abclonal), 1:50 diluted N- cad antibody (#610920, BD Biosciences), 1:50 diluted vimentin antibody (#A19607, Abclonal), 1:50 diluted Slug antibody (#TA800167, OriGene Technologies), and PBS control overnight at 4°C. .. Then, tissue arrays were incubated with an anti- rabbit- HRP polymer (ZSGB- Bio) for 20 min, followed by incubation with DAB (ZSGB- Bio) for 1 min.

    Article Title: Par6 Enhances Glioma Invasion by Activating MEK/ERK Pathway Through a LIN28/let-7d Positive Feedback Loop.
    Article Snippet: The invasion of glioblastoma usually results in the recurrence and poor prognosis in patients with glioma.. However, the underlying mechanisms involved in glioma invasion remains undefined.. In this study, immunohistochemistry analyses of glioma specimens demonstrated that high expression of Par6 was positively correlated with malignancy and poor prognosis of patients with glioma.

    Article Title: Deregulation of STING Signaling in Colorectal Carcinoma Constrains DNA-Damage Responses and Correlates With Tumorigenesis
    Article Snippet: Real-time PCR was performed with the TaqMan gene Expression Assay (Applied Biosystems). .. Tissue Microarray was purchased from Origene. ..

    Article Title: The Expression and Clinical Outcome of pCHK2-Thr68 and pCDC25C-Ser216 in Breast Cancer
    Article Snippet: .. Tumor cell analysis was conducted via large-core tissue microarray (TMA, OriGene, Rockville, MD, USA). ..

    Article Title: Glycerol-3-Phosphate Acyltranferase-2 Behaves as a Cancer Testis Gene and Promotes Growth and Tumorigenicity of the Breast Cancer MDA-MB-231 Cell Line
    Article Snippet: .. To examine the expression of GPAT2 protein in human breast cancer tissue, we performed immunohistochemistry on a tissue microarray (TMA) of human breast cancers (n = 36) or normal tissue (n = 6) (Origene, CT565863). ..

    Immunostaining:

    Article Title: Tissue‐specific expression and post‐transcriptional regulation of the ATPase inhibitory factor 1 (IF1) in human and mouse tissues
    Article Snippet: The intensity of the bands was quantified with a GS-900 Calibrated Densitometer (Bio-Rad) and ImageJ software (National Institutes of Health, Bethesda, MD, USA). .. Cancer Survey Tissue Microarrays (OriGene Technologies) containing 5 mm-width sections of formalin-fixed normal human tissues were used for immunostaining. ..

    Incubation:

    Article Title: BACH1 promotes the progression of esophageal squamous cell carcinoma by inducing the epithelial-mesenchymal transition and angiogenesis.
    Article Snippet: Tissue microarrays of ESCC, HEsoS105Su01 included 50 tumor tissues and paired esophageal epithelial tissues and ZH- ESC 77a included 77 tumor tissues, were purchased from Outdo Biotech and Zhuohao Medical Science and Technology (Shanghai, China). .. Slides of the tissue microarray were incubated with 1:200 diluted BACH1 antibody (#A5393, Abclonal), 1:50 diluted N- cad antibody (#610920, BD Biosciences), 1:50 diluted vimentin antibody (#A19607, Abclonal), 1:50 diluted Slug antibody (#TA800167, OriGene Technologies), and PBS control overnight at 4°C. .. Then, tissue arrays were incubated with an anti- rabbit- HRP polymer (ZSGB- Bio) for 20 min, followed by incubation with DAB (ZSGB- Bio) for 1 min.

    Control:

    Article Title: BACH1 promotes the progression of esophageal squamous cell carcinoma by inducing the epithelial-mesenchymal transition and angiogenesis.
    Article Snippet: Tissue microarrays of ESCC, HEsoS105Su01 included 50 tumor tissues and paired esophageal epithelial tissues and ZH- ESC 77a included 77 tumor tissues, were purchased from Outdo Biotech and Zhuohao Medical Science and Technology (Shanghai, China). .. Slides of the tissue microarray were incubated with 1:200 diluted BACH1 antibody (#A5393, Abclonal), 1:50 diluted N- cad antibody (#610920, BD Biosciences), 1:50 diluted vimentin antibody (#A19607, Abclonal), 1:50 diluted Slug antibody (#TA800167, OriGene Technologies), and PBS control overnight at 4°C. .. Then, tissue arrays were incubated with an anti- rabbit- HRP polymer (ZSGB- Bio) for 20 min, followed by incubation with DAB (ZSGB- Bio) for 1 min.

    Staining:

    Article Title: Par6 Enhances Glioma Invasion by Activating MEK/ERK Pathway Through a LIN28/let-7d Positive Feedback Loop.
    Article Snippet: The invasion of glioblastoma usually results in the recurrence and poor prognosis in patients with glioma.. However, the underlying mechanisms involved in glioma invasion remains undefined.. In this study, immunohistochemistry analyses of glioma specimens demonstrated that high expression of Par6 was positively correlated with malignancy and poor prognosis of patients with glioma.

    Software:

    Article Title: Par6 Enhances Glioma Invasion by Activating MEK/ERK Pathway Through a LIN28/let-7d Positive Feedback Loop.
    Article Snippet: The invasion of glioblastoma usually results in the recurrence and poor prognosis in patients with glioma.. However, the underlying mechanisms involved in glioma invasion remains undefined.. In this study, immunohistochemistry analyses of glioma specimens demonstrated that high expression of Par6 was positively correlated with malignancy and poor prognosis of patients with glioma.

    Cell Analysis:

    Article Title: The Expression and Clinical Outcome of pCHK2-Thr68 and pCDC25C-Ser216 in Breast Cancer
    Article Snippet: .. Tumor cell analysis was conducted via large-core tissue microarray (TMA, OriGene, Rockville, MD, USA). ..

    other:

    Article Title: Blocking AMPKαS496 phosphorylation improves mitochondrial dynamics and hyperglycemia in aging and obesity.
    Article Snippet: In brief Impaired mitochondrial dynamics causes serious health challenges.. Pearah et al. show that activated PKA or AKT by elevated glucagon or insulin directly phosphorylates AMPKaS496, leading to the impairment of AMPK-MFF signaling and mitochondrial dynamics.. Blocking AMPKaS496 phosphorylation by targeting peptides activates AMPK and improves mitochondrial dynamics and hyperglycemia in obesity and aging.

    Expressing:

    Article Title: Glycerol-3-Phosphate Acyltranferase-2 Behaves as a Cancer Testis Gene and Promotes Growth and Tumorigenicity of the Breast Cancer MDA-MB-231 Cell Line
    Article Snippet: .. To examine the expression of GPAT2 protein in human breast cancer tissue, we performed immunohistochemistry on a tissue microarray (TMA) of human breast cancers (n = 36) or normal tissue (n = 6) (Origene, CT565863). ..

    Immunohistochemistry:

    Article Title: Glycerol-3-Phosphate Acyltranferase-2 Behaves as a Cancer Testis Gene and Promotes Growth and Tumorigenicity of the Breast Cancer MDA-MB-231 Cell Line
    Article Snippet: .. To examine the expression of GPAT2 protein in human breast cancer tissue, we performed immunohistochemistry on a tissue microarray (TMA) of human breast cancers (n = 36) or normal tissue (n = 6) (Origene, CT565863). ..



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    Image Search Results


    Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

    Journal: The Journal of Biological Chemistry

    Article Title: Inhibition of cell surface GRP78 on brain tumors reverses drug resistance and stops cancer stem cell expansion

    doi: 10.1016/j.jbc.2026.111146

    Figure Lengend Snippet: Cell surface GRP78 colocalizes with ROR1, Cripto, and PD-L1 on late-stage patient GBM tissues but not on normal brain cerebrum tissues. A, three brain tissue microarrays with patient core tissue samples of GBM and normal tissues (#T174T from Biomax.us.) were stained for GBM markers. B, one microarray was stained with 4′,6-diamidino-2-phenylindole (DAPI) ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), ROR1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B2 (stage 4 GBM) and C7 (normal cerebrum) are pictured. C, a second microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. D, a third microarray was stained with DAPI ( blue -DNA), and antibodies to cell surface GRP78 ( green -FITC), PD-L1 ( red -PE). The last column is an overlay of all three stains to show colocalization. Cores B6 (stage 4 GBM) and C5 (normal cerebrum) are pictured. Scale bars represent 400 μm (10×). All images are from the same magnification of (10×) 400 μm. GBM, glioblastoma multiforme; GRP78, glucose-regulated protein 78; PD-L1, programed death-ligand 1; ROR1, receptor tyrosine kinase–like orphan receptor-1.

    Article Snippet: Briefly, three human brain tumors and normal brain tissue microarrays (T174c; Biomax. us) were deparaffinized with xylene and washed with PBS.

    Techniques: Staining, Microarray