hybridization chamber hybridization system-nimblegen systems (NimbleGen Systems GmbH)
90
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NimbleGen Systems GmbH
hybridization chamber hybridization system-nimblegen systems
Hybridization Chamber Hybridization System Nimblegen Systems, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nimblegen+microarray+scanner/hybridization+chamber+hybridization+system+nimblegen+systems/pmc09180418-135-2-5
Average 90 stars, based on 1 article reviews
Hybridization Chamber Hybridization System Nimblegen Systems, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/nimblegen+microarray+scanner/hybridization+chamber+hybridization+system+nimblegen+systems/pmc09180418-135-2-5
Average 90 stars, based on 1 article reviews
hybridization chamber hybridization system-nimblegen systems - by Bioz Stars,
2026-10
90/100 stars
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Hybridization:Article Title: LncRNA profile of glioblastoma reveals the potential role of lncRNAs in contributing to glioblastoma pathogenesis. Article Snippet: Double-strand cDNA (ds-cDNA) was synthesized from 5 μg of total RNA using an Invitrogen SuperScript ds-cDNA synthesis kit in the presence of 100 pmol oligo dT primers. ds-cDNA was cleaned and labeled in accordance with the NimbleGen Gene Expression Analysis protocol (NimbleGen Systems, Inc., Madison, WI, USA). .. Microarrays were hybridized at 42 ̊C for 16 to 20 h with 4 μg of Cy3 labelled ds-cDNA in NimbleGen hybridization buffer/hybridization component A in a Article Title: Microarray expression profile analysis of long non-coding RNAs of advanced stage human gastric cardia adenocarcinoma. Article Snippet: biopsy tissue from 12 GCa patients who were confirmed by pathology to have developed lymph node metastasis and 12 paired non-cancerous gastric cardia tissues to determine if a gene expression profile unique to the lymph node metastasis group could be detected.. Comparison of differentially expressed transcripts between the groups identified eight pathways that corresponded to down-regulated transcripts and 18 pathways that corresponded to up-regulated transcripts (p value cut-off 0.05).. Gene ontology analysis showed that the up-regulated transcripts were most highly enriched in SrP-dependent cotranslational protein targeting to membrane, cytosolic ribosome, and structural constituent of ribosome, and the down-regulated transcripts were highly enriched in carboxylic acid transport, focal adhesion, and cation binding. Article Title: Screening Analysis of Sirtuins Family Expression on Anti-Inflammation of Resveratrol in Endothelial Cells Article Snippet: Then, 100 pmol of deoxynucleoside triphosphates and 100 U of the Klenow fragment (New England Biolabs, USA) were added and the mix incubated at 37°C for 2 hours [15]. .. Microarrays were hybridized at 42°C during 16 to 20 hours with 4 μg of Cy3 labeled ds-cDNA in NimbleGen hybridization buffer/hybridization component A in a Article Title: Microarray expression profile of lncRNAs and the upregulated ASLNC04080 lncRNA in human endometrial carcinoma. Article Snippet: Then, 100 pmol of deoxynucleoside triphosphates and 100 units of the Klenow fragment (New England Biolabs, Ipswich, MA, USA) were added and the mix incubated at 37 ̊C for 2 h. The reaction was stopped by adding 0.1 volume of 0.5 M EDTA, and the labeled ds-cDNA was purified by isopropanol/ethanol precipitation. .. Microarrays were hybridized at 42 ̊C for 16-20 h with 4 μg of Cy3 labelled ds-cDNA in Nimblegen hybridization buffer/hybridization component A in a Article Title: Alteration of Gene Expression, DNA Methylation, and Histone Methylation in Free Radical Scavenging Networks in Adult Mouse Hippocampus following Fetal Alcohol Exposure Article Snippet: .. Microarrays were hybridized at 42°C during 16 to 20h with Cy3/5 labelled DNA in NimbleGen hybridization buffer/ hybridization component A in a Article Title: Octacosanol Modifies Obesity, Expression Profile and Inflammation Response of Hepatic Tissues in High-Fat Diet Mice Article Snippet: One OD Cy3 primer was incubated with 1 μg of ds-cDNA for 10 min at 98 °C, followed by addition of 100 pmol of deoxynucleoside triphosphates and 100 U of the Klenow enzyme (New England Biolabs, Ipswich, MA, USA), and incubation at 37 °C for 2 h. Thereafter, the reaction was terminated by adding 0.1 vol of 0.5 M EDTA, and the purified ds-cDNA was precipitated with isopropanol/ethanol. .. In a Article Title: DNA methylation changes in the hippocampus of learning and memory disorder offspring rats of lead exposure during pregnant and lactation period. Article Snippet: Then, 100 U of the Klenow fragment (New England Biolabs, USA) and 100 pmol of deoxynucleoside triphosphates were added, and the mixture was incubated at 37 °C for 2 h. Adding 0.1 volume of 0.5 M EDTA to stopped the reaction, and purified labeled DNA with isopropanol/ethanol precipitation. .. Microarrays were hybridized at 42 °C for 16 to 20 h with Cy3/5 labeled DNA in Nimblegen hybridization buffer/hybridization component A in a Labeling:Article Title: Screening Analysis of Sirtuins Family Expression on Anti-Inflammation of Resveratrol in Endothelial Cells Article Snippet: Then, 100 pmol of deoxynucleoside triphosphates and 100 U of the Klenow fragment (New England Biolabs, USA) were added and the mix incubated at 37°C for 2 hours [15]. .. Microarrays were hybridized at 42°C during 16 to 20 hours with 4 μg of Cy3 labeled ds-cDNA in NimbleGen hybridization buffer/hybridization component A in a Article Title: DNA methylation changes in the hippocampus of learning and memory disorder offspring rats of lead exposure during pregnant and lactation period. Article Snippet: Then, 100 U of the Klenow fragment (New England Biolabs, USA) and 100 pmol of deoxynucleoside triphosphates were added, and the mixture was incubated at 37 °C for 2 h. Adding 0.1 volume of 0.5 M EDTA to stopped the reaction, and purified labeled DNA with isopropanol/ethanol precipitation. .. Microarrays were hybridized at 42 °C for 16 to 20 h with Cy3/5 labeled DNA in Nimblegen hybridization buffer/hybridization component A in a Microarray:Article Title: Octacosanol Modifies Obesity, Expression Profile and Inflammation Response of Hepatic Tissues in High-Fat Diet Mice Article Snippet: One OD Cy3 primer was incubated with 1 μg of ds-cDNA for 10 min at 98 °C, followed by addition of 100 pmol of deoxynucleoside triphosphates and 100 U of the Klenow enzyme (New England Biolabs, Ipswich, MA, USA), and incubation at 37 °C for 2 h. Thereafter, the reaction was terminated by adding 0.1 vol of 0.5 M EDTA, and the purified ds-cDNA was precipitated with isopropanol/ethanol. .. In a |