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aqi 3d deconvolution module  (Nikon)


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    Structured Review

    Nikon aqi 3d deconvolution module
    Aqi 3d Deconvolution Module, supplied by Nikon, used in various techniques. Bioz Stars score: 95/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nikon+elements+deconvolution+software/Maximum+Magnification+Module/pmc06449396-277-27-32
    Average 95 stars, based on 311 article reviews
    aqi 3d deconvolution module - by Bioz Stars, 2026-09
    95/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
    Article Snippet: .. Samples were loaded onto an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

    Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
    Article Snippet: .. Samples were imaged using an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

    Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
    Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

    Fluorescence:

    Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
    Article Snippet: .. Samples were loaded onto an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

    Article Title: Methods for multiplex imaging using labeled nucleic acid imaging agents
    Article Snippet: .. Samples were imaged using an inverted Nikon Eclipse Ti microscope (Nikon Instruments) with a fluorescence module and an Andor Zyla sCMOS camera. ..

    Software:

    Article Title: Amylin exacerbates tau pathology in the visual cortex of diabetic mice by impairing lysosomal activity
    Article Snippet: .. High-magnification z-stack images 265 were converted to maximum intensity 2D projections and deconvolved in NIS-Elements 266 software (Nikon) 52. ..

    Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
    Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

    Imaging:

    Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
    Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

    Immunofluorescence:

    Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
    Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.

    Activation Assay:

    Article Title: Enhancing Lateral Resolution Using Two-Colour Direct Stochastic Optical Reconstruction Microscopy to Unravel Synaptic Tau Pathology in Alzheimer's Disease.
    Article Snippet: Aims: In Alzheimer's disease (AD), the pathological accumulation of tau in synapses contributes to synapse dysfunction and loss.. However, the small and complex structure of synapses limits the investigation when using conventional techniques.. In this work, we describe the combination of array tomography (AT) with twocolour direct stochastic optical reconstruction microscopy (dSTORM) to enhance lateral resolution for resolving synaptic terminals in human postmortem brain.



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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D <t>widefield-deconvolution</t> microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).
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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D <t>widefield-deconvolution</t> microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).
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    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D <t>widefield-deconvolution</t> microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).
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    Image Search Results


    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Journal: bioRxiv

    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics

    doi: 10.1101/2023.06.03.543566

    Figure Lengend Snippet: A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 30 minutes. Images were obtained using 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and Rab11 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with Rab11-positive endosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Article Snippet: The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level).

    Techniques: Microscopy

    A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 60 minutes. Cells were visualized by 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and LAMP1 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with LAMP1-positive lysosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Journal: bioRxiv

    Article Title: Sialylation of EGFR by ST6GAL1 induces receptor activation and modulates trafficking dynamics

    doi: 10.1101/2023.06.03.543566

    Figure Lengend Snippet: A). Maximum intensity projection and 3D volume projection images for OV4 EV and OE cells treated with or without EGF for 60 minutes. Cells were visualized by 3D widefield-deconvolution microscopy. The images depict the distribution of EGFR (green) and LAMP1 (magenta) obtained following the processing of the acquired widefield 3D Z-stack images by the Richardson-Lucy algorithm for deconvolution. Scale bar for the field of view (FOV) = 20 μm, region of interest (ROI) = 5 μm. B). Quantification of the fraction of EGFR co-localized with LAMP1-positive lysosomes was executed using the JACoP plugin in Fiji. Graphs depict mean +/− S.D. from two independent experiments with 20 cells analyzed per group, per experiment. Data were analyzed by one way ANOVA with Tukey’s test (ns: p > 0.05, ****: p < 0.0001).

    Article Snippet: The widefield Z-stack images were deconvolved using Nikon Elements deconvolution software (Richardson Lucy; parameters: 50 iterations, low noise level).

    Techniques: Microscopy