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Genisphere llc ncode mirna microarray controls
Ncode Mirna Microarray Controls, supplied by Genisphere llc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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other:

Article Title: MicroRNAs are differentially expressed in ulcerative colitis and alter expression of macrophage inflammatory peptide-2 alpha.
Article Snippet: d I i c P f ackground & Aims: Chronic inflammatory bowel iseases such as ulcerative colitis (UC) are associated ith differential expression of genes involved in nflammation and tissue remodeling.. MicroRNAs miRNAs), which direct mRNA degradation and ranslational inhibition, influence a number of disase processes.. We examined whether miRNAs are ifferentially expressed in UC tissues and are associted with expression of genes that regulate inflammaion.

Microarray:

Article Title: Identification of MicroRNAs Associated with Ileal and Colonic Crohn's Disease
Article Snippet: .. Briefly, 500 ng of small RNAs, mixed with NCode miRNA Microarray Controls, were labeled with Oyster-550 or Oyster-650 using the Flashtag RNA labeling kit (Genisphere, Hatfield, PA). ..

Labeling:

Article Title: Identification of MicroRNAs Associated with Ileal and Colonic Crohn's Disease
Article Snippet: .. Briefly, 500 ng of small RNAs, mixed with NCode miRNA Microarray Controls, were labeled with Oyster-550 or Oyster-650 using the Flashtag RNA labeling kit (Genisphere, Hatfield, PA). ..



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Image Search Results


(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

Journal: bioRxiv

Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

doi: 10.1101/2025.10.27.684728

Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E) BRET values represent the proximity between Nluc-OMM, TOM20-Nluc, respectively, and localization sensors (YFP), n =5. (F) Schematic representation of mitochondrial protein sensors in different compartments. (G) BRET values represent the proximity between TOM20-Nluc and mitochondrial proteins (YFP), n =4

Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

Techniques: Immunofluorescence, Staining

(A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

Journal: bioRxiv

Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

doi: 10.1101/2025.10.27.684728

Figure Lengend Snippet: (A) Immunofluorescence images showing mito-biosensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Images showing colocalization of mito-sensors tagged to Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of mito-biosensors in different compartments. (D,E,F) BRET values represent the proximity between CLU-Nluc, IBM-Nluc, and MICU1-Nluc, respectively, and localization sensors (YFP), n =5. (G) Schematic representation of the localization of VDAC1 and localization biosensors in different compartments. (H) BRET values representing the proximity between VDAC1-Nluc and loc-sensors (YFP), n = 4.

Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

Techniques: Immunofluorescence, Staining

(A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

Journal: bioRxiv

Article Title: BRET-Based Mitochondrial Subcompartment Localization Biosensors

doi: 10.1101/2025.10.27.684728

Figure Lengend Snippet: (A) Immunofluorescence images showing localization of mito-sensors tagged to a YFP (Green) with endogenous TOM20 staining (red) and the superposition of both acquisitions with Dapi (blue). (B) Merged images showing localization of mito-sensors tagged to a Nluc (Green) with Mito Tracker Red CMX Ros staining (Red). (C) Schematic representation of localization sensors in different compartments. (D, E, F) BRET values represent the proximity between -MX-Nluc, ATP5F1C-Nluc, and OTC-Nluc, respectively, and localization sensors (YFP). n =5. (G) Schematic representation of the localization of the Sirtuins and MX loc-biosensor in different compartments. (G) BRET values represent the proximity between MX-Nluc, MX-YFP, and sirtuins (YFP), n = 4

Article Snippet: TOM20-YFP(Nluc) fusion proteins were obtained by fusing the TOM20 coding region (Addgene # 55146) in its c-terminus to YFP or Nluc.

Techniques: Immunofluorescence, Staining