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multiscript rt kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher multiscript rt kit
    Multiscript Rt Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscript+rt/RevertAid+Reverse+Transcriptase+(200+U%2F%C2%B5L)/pm38701775-361-5-8
    Average 90 stars, based on 1 article reviews
    multiscript rt kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    other:

    Article Title: ETTIN-mediated auxin signalling is an angiosperm-specific neofunctionalization for carpel development
    Article Snippet: The on-column RNAse free DNAse kit (Qiagen) was added to remove genomic DNA and the SuperScriptTM IV First-Strand Synthesis kit (ThermoFisher) was used according to synthesise cDNA from 1 μg of total RNA.

    Article Title: A guanidine-based coronavirus replication inhibitor which targets the nsp15 endoribonuclease and combines a direct antiviral effect with subtle boosting of interferon
    Article Snippet: To measure the mRNA levels for a selection of innate immune factors, the cellular RNA extracts were submitted to RT-qPCR (High-Capacity cDNA Reverse Transcription Kit, Applied Biosystems), using random primers for cDNA synthesis and gene-specific primers (primer sequences are listed in S3 table) for qPCR (GoTaq qPCR kit, Promega).

    Article Title: Mitigating aging and doxorubicin induced bone loss in mature mice via mechanobiology based treatments.
    Article Snippet: Aging leads to a reduced anabolic response to mechanical stimuli and a loss of bone mass and structural integrity.. Chemotherapy agents such as doxorubicin exacerbate the degeneration of aging skeleton and further subject older cancer patients to a higher fracture risk.. To alleviate this clinical problem, we proposed and tested a novel mechanobiology-based therapy.

    Article Title: Identification of M-Sec as a unique cellular regulator of CSF-1 receptor activation
    Article Snippet: In brief, RNA was isolated using ISOGEN II reagent (Nippon Gene, Tokyo, Japan), cDNA was prepared using M-MLV RT (Invitrogen), and real-time PCR was performed using SYBR Premix Ex Taq II (TaKaRa-Bio) and LightCycler (Roche). β-actin mRNA was quantified as an internal control.

    Expressing:

    Article Title: Epigenetic effects induced by silver nanoparticles in Caenorhabditis elegans after multigenerational exposure
    Article Snippet: .. Its expression levels were also stable among all Ag treatments used in this study with 0.5–1Ct difference ( Fig. S2 ). cDNA synthesis was carried out with 500 ng of total RNA by using RevertAid First Strand Synthesis Kit (ThermoFisher Scientific, Vilnius, Lithuania). qRT-PCR reactions were carried out in 10 μL volumes with TaqMan fast advanced master mix, TaqMan gene expression assays for each gene, and cDNA diluted 1:100 ( spr-5,set-17,set-30 ) or 1:19 ( jmjd-2, set-25, set-26 ). ..

    cDNA Synthesis:

    Article Title: Epigenetic effects induced by silver nanoparticles in Caenorhabditis elegans after multigenerational exposure
    Article Snippet: .. Its expression levels were also stable among all Ag treatments used in this study with 0.5–1Ct difference ( Fig. S2 ). cDNA synthesis was carried out with 500 ng of total RNA by using RevertAid First Strand Synthesis Kit (ThermoFisher Scientific, Vilnius, Lithuania). qRT-PCR reactions were carried out in 10 μL volumes with TaqMan fast advanced master mix, TaqMan gene expression assays for each gene, and cDNA diluted 1:100 ( spr-5,set-17,set-30 ) or 1:19 ( jmjd-2, set-25, set-26 ). ..

    Article Title: Mutational cooperativity of RUNX1::RUNX1T1 isoform 9a and oncogenic NRAS in zebrafish myeloid leukaemia
    Article Snippet: The purified RNA was then Dnase-treated using ezDNase Enzyme (Invitrogen). .. A portion of Dnase-treated RNA was kept aside as a “No RT” control, while the remainder was used in first strand cDNA synthesis reactions with Superscript IV (Invitrogen), priming with random hexamers oligos (Invitrogen). .. PCR was performed using the KAPA2G Robust HotStart PCR Kit (Kapabiosystems) with 2 μl of cDNA synthesis reaction (+RT) or No RT template, per PCR reaction and the gene-specific primers for 9a , NRAS (this study) or ef1a ( ) ( ).

    Quantitative RT-PCR:

    Article Title: Epigenetic effects induced by silver nanoparticles in Caenorhabditis elegans after multigenerational exposure
    Article Snippet: .. Its expression levels were also stable among all Ag treatments used in this study with 0.5–1Ct difference ( Fig. S2 ). cDNA synthesis was carried out with 500 ng of total RNA by using RevertAid First Strand Synthesis Kit (ThermoFisher Scientific, Vilnius, Lithuania). qRT-PCR reactions were carried out in 10 μL volumes with TaqMan fast advanced master mix, TaqMan gene expression assays for each gene, and cDNA diluted 1:100 ( spr-5,set-17,set-30 ) or 1:19 ( jmjd-2, set-25, set-26 ). ..

    Gene Expression:

    Article Title: Epigenetic effects induced by silver nanoparticles in Caenorhabditis elegans after multigenerational exposure
    Article Snippet: .. Its expression levels were also stable among all Ag treatments used in this study with 0.5–1Ct difference ( Fig. S2 ). cDNA synthesis was carried out with 500 ng of total RNA by using RevertAid First Strand Synthesis Kit (ThermoFisher Scientific, Vilnius, Lithuania). qRT-PCR reactions were carried out in 10 μL volumes with TaqMan fast advanced master mix, TaqMan gene expression assays for each gene, and cDNA diluted 1:100 ( spr-5,set-17,set-30 ) or 1:19 ( jmjd-2, set-25, set-26 ). ..

    Synthesized:

    Article Title: Active protein ubiquitination regulates xylem vessel functionality
    Article Snippet: The concentration and quality of the RNA were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA was synthesized from 1 μ g DNase-treated RNA by reverse transcription using oligo(dT)18 primers (Invitrogen) and SuperScript IV (Invitrogen). .. Quantitative PCR was carried out using a Light Cycler 480 system (Roche) with TB Green Premix EX Taq II (TaKaRa).

    Reverse Transcription:

    Article Title: Active protein ubiquitination regulates xylem vessel functionality
    Article Snippet: The concentration and quality of the RNA were determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific). .. First-strand cDNA was synthesized from 1 μ g DNase-treated RNA by reverse transcription using oligo(dT)18 primers (Invitrogen) and SuperScript IV (Invitrogen). .. Quantitative PCR was carried out using a Light Cycler 480 system (Roche) with TB Green Premix EX Taq II (TaKaRa).

    Article Title: Analysis of transcriptional response in haploid and diploid Schizosaccharomyces pombe under genotoxic stress
    Article Snippet: .. Total RNA was reverse transcribed with SuperScript III (Invitrogen) using the oligo dT and random hexamer protocol for mRNA and rRNA targets, respectively. ..

    Control:

    Article Title: Mutational cooperativity of RUNX1::RUNX1T1 isoform 9a and oncogenic NRAS in zebrafish myeloid leukaemia
    Article Snippet: The purified RNA was then Dnase-treated using ezDNase Enzyme (Invitrogen). .. A portion of Dnase-treated RNA was kept aside as a “No RT” control, while the remainder was used in first strand cDNA synthesis reactions with Superscript IV (Invitrogen), priming with random hexamers oligos (Invitrogen). .. PCR was performed using the KAPA2G Robust HotStart PCR Kit (Kapabiosystems) with 2 μl of cDNA synthesis reaction (+RT) or No RT template, per PCR reaction and the gene-specific primers for 9a , NRAS (this study) or ef1a ( ) ( ).

    Random Hexamer:

    Article Title: Analysis of transcriptional response in haploid and diploid Schizosaccharomyces pombe under genotoxic stress
    Article Snippet: .. Total RNA was reverse transcribed with SuperScript III (Invitrogen) using the oligo dT and random hexamer protocol for mRNA and rRNA targets, respectively. ..



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