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taqman microrna reverse transcription kit  (Thermo Fisher)


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    Structured Review

    Thermo Fisher taqman microrna reverse transcription kit
    Taqman Microrna Reverse Transcription Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10732 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+kit/TaqMan+MicroRNA+Cells-to-CT+Kit/pmc05856835-50-15-20
    Average 99 stars, based on 10732 article reviews
    taqman microrna reverse transcription kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Isolation:

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: .. At 72h post-transfection, microRNA (miRNA) was isolated using miRNeasy Mini Kit (Cat#: 217004, Qiagen) and reverse transcribed using TaqMan MicroRNA Reverse Transcription Kit (Cat#: 4366596, Thermo Fisher Scientific) according to the manufacturer’s instructions. miRNA was isolated from lung mECs of Pde2a flox controls and Pde2a flox ; Cdh5-CreER T2 mutants and processed similarly. miR-139-5p expression levels were analyzed using TaqMan MicroRNA Assay (Cat#: 4427975, Thermo Fisher Scientific) and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). .. Relative microRNA expression levels were normalized to RNU48 (assay ID: 001006) for HDLECs, or snoRNA202 (assay ID: 001232) and snoRNA234 (assay ID: 001234) for mECs (all from Thermo Fisher Scientific) ( ).

    Reverse Transcription:

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: .. At 72h post-transfection, microRNA (miRNA) was isolated using miRNeasy Mini Kit (Cat#: 217004, Qiagen) and reverse transcribed using TaqMan MicroRNA Reverse Transcription Kit (Cat#: 4366596, Thermo Fisher Scientific) according to the manufacturer’s instructions. miRNA was isolated from lung mECs of Pde2a flox controls and Pde2a flox ; Cdh5-CreER T2 mutants and processed similarly. miR-139-5p expression levels were analyzed using TaqMan MicroRNA Assay (Cat#: 4427975, Thermo Fisher Scientific) and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). .. Relative microRNA expression levels were normalized to RNU48 (assay ID: 001006) for HDLECs, or snoRNA202 (assay ID: 001232) and snoRNA234 (assay ID: 001234) for mECs (all from Thermo Fisher Scientific) ( ).

    Article Title: miR-132-3p and KLF7 as novel regulators of aortic stiffening-associated EndMT in type 2 diabetes mellitus
    Article Snippet: Total microRNA was isolated using a TRIzol Reagent (Ambion, Austin, TX, USA) according to the manufacturer’s protocol. .. Reverse transcription was performed by using the TaqMan microRNA Reverse Transcription kit (Applied Biosystems, Foster City, CA, USA) and microRNA-specific stemloop primers (Table ). .. For miR-qRT-PCR, analysis was performed on a mixture containing 5 ng cDNA equivalent, 0.1 μM sense primer, 0.1 μM antisense primer (Table ) and Fast SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA).

    Article Title: The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand cleavage in C. elegans
    Article Snippet: .. The following reagents were used: Alt-R crRNAs (IDT), Cas9 (IDT), Trizol (ThermoFisher), TaqMan MicroRNA Reverse Transcription kit (ThermoFisher), Taqman miRNA probes (ThermoFisher), Taqman Universal Mastermix II with UNG (ThermoFisher), NEBNext Small RNA Library Prep kit (NEB), RNA oligonucleotides (Horizon Discoveries). .. The following specialized instruments were used: QuantStudio Pro 6 (Applied Biosystems), HiSeq 3000 (Illumina), NextSeq 550 (Illumina), NovaSeq6000 S4 (Illumina).

    Article Title: The catalytic activity of microRNA Argonautes plays a modest role in microRNA star strand cleavage in C. elegans
    Article Snippet: .. For each sample, 9.96ng of total RNA was used to perform 5μL reverse transcription reactions using the TaqMan MicroRNA Reverse Transcription kit (ThermoFisher). .. After RT, 1.33μL of 1:4-diluted RT reaction was used in 5μL qPCR reactions with Taqman miRNA probes and Taqman Universal Mastermix II with UNG (ThermoFisher).

    Article Title: Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing
    Article Snippet: EV RNA was isolated with a SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to manufacturer’s instructions. .. RNA integrity was determined by an Agilent 2100 bioanalyzer. cDNA of EV miRNA was synthesized from 10 ng total RNA using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). .. The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems. hsa-miR-423-3p was used as an endogenous reference miRNA, as it was identified as one of the most abundant and stable miRNAs in ASC EVs by our small RNA-sequencing analysis.

    Article Title: WNT5A, β-catenin and SUFU expression patterns, and the significance of microRNA deregulation in placentas with intrauterine growth restriction
    Article Snippet: For microRNA analysis, total RNA was isolated from five consecutive 5-μm thick sections of FFPE control placental tissue (n=14) and IUGR placental tissue (n=14) as described above and purified with the ‘NucleoSpin miRNA Plasma̛ kit (Macherey-Nagel, Germany). .. Reverse transcription was performed using the ‘TaqMan MicroRNA Reverse Transcription Kit’ (Applied Biosystems) and specific loop primers for Hsa-miR-214-3p (assay ID 002306, Applied Biosystems, USA) and Has-miR-378a-5p (assay ID 000567, Applied Biosystems, USA) following the manufacturer's procedure. .. The expression levels of targeted miRNAs were determined using the ‘TaqMan microRNA Assay’ and ‘TaqManTM Universal Master Mix II, no UNG’ (Thermo Fisher Scientific, Inc.) following the manufacturer's instructions.

    Article Title: miR-132-3p and KLF7 as novel regulators of aortic stiffening-associated EndMT in type 2 diabetes mellitus
    Article Snippet: Total microRNA was isolated using a TRIzol-based (Invitrogen, Carlsbad, CA, USA) RNA isolation protocol. .. Reverse transcription was performed by using the TaqMan microRNA Reverse Transcription kit (Applied Biosystems, Foster Biosystems, Foster City, USA) according to the manufacturer’s instructions. .. MicroRNA TaqMan assays (Applied Biosystems, Foster City, USA) for hsa-miR-132-3p was used.

    Expressing:

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: .. At 72h post-transfection, microRNA (miRNA) was isolated using miRNeasy Mini Kit (Cat#: 217004, Qiagen) and reverse transcribed using TaqMan MicroRNA Reverse Transcription Kit (Cat#: 4366596, Thermo Fisher Scientific) according to the manufacturer’s instructions. miRNA was isolated from lung mECs of Pde2a flox controls and Pde2a flox ; Cdh5-CreER T2 mutants and processed similarly. miR-139-5p expression levels were analyzed using TaqMan MicroRNA Assay (Cat#: 4427975, Thermo Fisher Scientific) and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). .. Relative microRNA expression levels were normalized to RNU48 (assay ID: 001006) for HDLECs, or snoRNA202 (assay ID: 001232) and snoRNA234 (assay ID: 001234) for mECs (all from Thermo Fisher Scientific) ( ).

    TaqMan microRNA Assay:

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: .. At 72h post-transfection, microRNA (miRNA) was isolated using miRNeasy Mini Kit (Cat#: 217004, Qiagen) and reverse transcribed using TaqMan MicroRNA Reverse Transcription Kit (Cat#: 4366596, Thermo Fisher Scientific) according to the manufacturer’s instructions. miRNA was isolated from lung mECs of Pde2a flox controls and Pde2a flox ; Cdh5-CreER T2 mutants and processed similarly. miR-139-5p expression levels were analyzed using TaqMan MicroRNA Assay (Cat#: 4427975, Thermo Fisher Scientific) and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). .. Relative microRNA expression levels were normalized to RNU48 (assay ID: 001006) for HDLECs, or snoRNA202 (assay ID: 001232) and snoRNA234 (assay ID: 001234) for mECs (all from Thermo Fisher Scientific) ( ).

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The phosphodiesterase 2A regulates lymphatic endothelial development via cGMP-mediated control of Notch signaling
    Article Snippet: .. At 72h post-transfection, microRNA (miRNA) was isolated using miRNeasy Mini Kit (Cat#: 217004, Qiagen) and reverse transcribed using TaqMan MicroRNA Reverse Transcription Kit (Cat#: 4366596, Thermo Fisher Scientific) according to the manufacturer’s instructions. miRNA was isolated from lung mECs of Pde2a flox controls and Pde2a flox ; Cdh5-CreER T2 mutants and processed similarly. miR-139-5p expression levels were analyzed using TaqMan MicroRNA Assay (Cat#: 4427975, Thermo Fisher Scientific) and a TaqMan Universal Master Mix II no UNG with a StepOnePlus RT PCR system (Applied Biosystems). .. Relative microRNA expression levels were normalized to RNU48 (assay ID: 001006) for HDLECs, or snoRNA202 (assay ID: 001232) and snoRNA234 (assay ID: 001234) for mECs (all from Thermo Fisher Scientific) ( ).

    other:

    Article Title: ciRS-7-miR7 regulate ischemia induced neuronal death via glutamatergic signaling
    Article Snippet: Mature microRNAs were reverse-transcribed with TaqManTM MicroRNA Reverse Transcription Kit (Invitrogen) using miRNA-specific primers supplied in the TaqMan® probe kit for qPCR following the manufacturer’s protocol.

    Synthesized:

    Article Title: Extracellular Vesicles from Inflammation-Primed Adipose-Derived Stem Cells Enhance Achilles Tendon Repair by Reducing Inflammation and Promoting Intrinsic Healing
    Article Snippet: EV RNA was isolated with a SeraMir Exosome RNA Column Purification Kit (System Biosciences) according to manufacturer’s instructions. .. RNA integrity was determined by an Agilent 2100 bioanalyzer. cDNA of EV miRNA was synthesized from 10 ng total RNA using a TaqMan MicroRNA Reverse Transcription Kit (Applied Biosystems) and primers specific for mmu-miR-147-3p (alias, hsa-miR-147b; Applied Biosystems). .. The relative abundance of EV miRNA was determined by TaqMan PCR using TaqMan Universal PCR Master Mix II and TaqMan primers and probes from Applied Biosystems. hsa-miR-423-3p was used as an endogenous reference miRNA, as it was identified as one of the most abundant and stable miRNAs in ASC EVs by our small RNA-sequencing analysis.



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