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reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher reverse transcriptase
    Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 888 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+kit/Single+Cell-to-CT+qRT-PCR+Kit/pmc02891459-102-29-35
    Average 98 stars, based on 888 article reviews
    reverse transcriptase - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Expressing:

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCodeTM miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    cDNA Synthesis:

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCodeTM miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Quantitative RT-PCR:

    Article Title: Oxidative Stress Down-Regulates MiR-20b-5p, MiR-106a-5p and E2F1 Expression to Suppress the G1/S Transition of the Cell Cycle in Multipotent Stromal Cells
    Article Snippet: Total RNA was isolated from WJ-MSC using RNeasy Plus Mini Kit (Qiagen, CA, USA). .. For miRNA expression analysis, miRNA polyadenylation was first performed using 1 μg total RNA, followed by first-strand cDNA synthesis using the NCodeTM miRNA first-strand cDNA synthesis and qRT-PCR kit (Invitrogen, CA, USA). .. Ten-fold diluted cDNA was used for quantitative RT-PCR using a SYBR select master mix kit (Applied Biosystems, TX, USA) in a Rotor-Gene Q (Qiagen) thermal cycler.

    Article Title: Vibrio cholerae Cytolysin Causes an Inflammatory Response in Human Intestinal Epithelial Cells That Is Modulated by the PrtV Protease
    Article Snippet: .. The 18S rRNA content was determined in all samples using a real-time quantitative RT-PCR kit (Applied Biosystems) and results were expressed as mRNA copies per 18S rRNA unit. .. Concentrations of IL-8 and TNF-α proteins were determined in duplicates of tissue culture medium collected from the lower chamber of monolayer cultures by using the Endogen Human IL-8 and TNF-α ELISA Kits, respectively (Pierce Biotechnology, Rockford, IL).

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Article Title: Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1)
    Article Snippet: .. Total RNA was extracted from peripheral blood with commercially available kits (Qiagen, Hilden, Germany), and cDNA synthesized from 1 μ g of RNA with SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR Kit (Invitrogen) according to manufacturer's instructions. .. NPSR1 messenger RNA (mRNA) expression levels were measured by performing quantitative real-time PCR in an ABI Prism 7500 Fast Sequence Detection System (Applied Biosystems, Foster City, CA, USA) with SYBRGreen mix according to the manufacturer's instructions.

    Comparison:

    Article Title: miR-10a is aberrantly overexpressed in Nucleophosmin1 mutated acute myeloid leukaemia and its suppression induces cell death
    Article Snippet: .. To confirm miR-10a expression in primary samples and comparison of expression in cell lines, 1 μg of total RNA was used for cDNA synthesis reaction using the NCode microRNA first strand synthesis and qRT-PCR kit (Invitrogen, USA). .. Quantitative real time polymerase chain reaction (qRT-PCR) was performed using the Platinum Sybr Green Taq (Invitrogen, USA) and the Rotorgene RG-3000 thermocycler (Qiagen, Hilden, Germany).

    other:


    Spectrophotometry:

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Reverse Transcription:

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Article Title: Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification
    Article Snippet: Total RNA was extracted from cells using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instruction. .. Three μ g of RNA from each sample was then reverse-transcribed into first-strand cDNA in 20 μ L of reaction mixture using the SuperScript First-Strand Synthesis System with the Real-time PCR kit (Invitrogen). .. Measurements were performed by an ABI PRISM 9700 HT sequence detection system (Applied Biosystems, Foster City, CA) using a predeveloped Taqman probe/primer combination for M1-related genes and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from the same cDNA samples.

    Real-time Polymerase Chain Reaction:

    Article Title: Pseudomonas aeruginosa Siderophores Damage Lung Epithelial Cells and Promote Inflammation
    Article Snippet: .. Total RNA yield was quantified on a Nanodrop spectrophotometer (ThermoFisher Scientific, Waltham, MA). cDNA synthesis was performed on a Bio-RAD T100 Thermo Cycler (Bio-RAD, Hercules, CA) using a reverse transcription kit (Applied Biosystems, Waltham, MA). qRT-PCR was performed on a Bio-RAD CFX Connect Real-Time System (Bio-RAD, Hercules, CA) using a universal qPCR master mix (New England Biolabs, Ipswich, MA). qPCR primer sequences are available upon request. ..

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Article Title: Effects of Low-Level Laser Therapy on M1-Related Cytokine Expression in Monocytes via Histone Modification
    Article Snippet: Total RNA was extracted from cells using TRIzol (Invitrogen, Carlsbad, CA) according to the manufacturer's instruction. .. Three μ g of RNA from each sample was then reverse-transcribed into first-strand cDNA in 20 μ L of reaction mixture using the SuperScript First-Strand Synthesis System with the Real-time PCR kit (Invitrogen). .. Measurements were performed by an ABI PRISM 9700 HT sequence detection system (Applied Biosystems, Foster City, CA) using a predeveloped Taqman probe/primer combination for M1-related genes and glyceraldehyde 3-phosphate dehydrogenase (G3PDH) from the same cDNA samples.

    Polymerase Chain Reaction:

    Article Title: Identification of an unrecognized circRNA associated with development of renal fibrosis
    Article Snippet: The HK-2 cells were transfected with siRNAs or plasmids for 24 h using the Lipofectamine RNAi MAX transfection kit (Invitrogen, United States). .. The total RNAs were reverse-transcribed into complementary cDNA by RNA PCR Kit (TaKaRa, Dalian, China), and qRT-PCR was performed using the SYBR Premix Ex Taq Kit (Takara) and the 7,500 Real-time PCR System (Applied Biosystems, Foster, CA, United States) according to the manufacturer’s instructions. ..

    Synthesized:

    Article Title: Multiple Polymorphisms Affect Expression and Function of the Neuropeptide S Receptor (NPSR1)
    Article Snippet: .. Total RNA was extracted from peripheral blood with commercially available kits (Qiagen, Hilden, Germany), and cDNA synthesized from 1 μ g of RNA with SuperScriptTM III First-Strand Synthesis SuperMix for qRT-PCR Kit (Invitrogen) according to manufacturer's instructions. .. NPSR1 messenger RNA (mRNA) expression levels were measured by performing quantitative real-time PCR in an ABI Prism 7500 Fast Sequence Detection System (Applied Biosystems, Foster City, CA, USA) with SYBRGreen mix according to the manufacturer's instructions.



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