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high-capacity cdna reverse transcription kit with multiscribe reverse transcriptase  (Thermo Fisher)


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    Structured Review

    Thermo Fisher high-capacity cdna reverse transcription kit with multiscribe reverse transcriptase
    High Capacity Cdna Reverse Transcription Kit With Multiscribe Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiscribe+reverse+transcriptase+kit/multiscribe+high+capacity+cdna+reverse+transcriptase+kit/pm39779854-658-25-28
    Average 90 stars, based on 1 article reviews
    high-capacity cdna reverse transcription kit with multiscribe reverse transcriptase - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: The Immuno-Suppressive Effects of Cyclic, Environmental Heat Stress in Broiler Chickens: Local and Systemic Inflammatory Responses to an Intradermal Injection of Lipopolysaccharide.
    Article Snippet: Total RNA quality and concentration were determined using NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. RNA was transcribed to cDNA using the High-Capacity cDNA kit with MultiScribe Reverse Transcriptase following the manufacturer’s protocol (Thermo Fisher Scientific). ..

    Article Title: Potential Anti-Inflammatory and Chondroprotective Effect of Luzula sylvatica .
    Article Snippet: Total RNA was collected using Trizol (Invitrogen, Thermo Fisher Scientific) and treated with DNAse I (Invitrogen, Thermo Fisher Scientific) to remove genomic DNA. .. The Reverse transcriptase (RT) was then carried out with the MultiScribe reverse transcriptase (High Capacity cDNA Reverse Transcription Kit, Applied Biosystems, Thermo Fisher Scientific) using the StepOne apparatus (StepOne Real-Time PCR System, Applied Biosystems). .. The analysis of IL1β, MMP1, MMP2 and ADAMTS4 expression in the monolayers of NHAC-kn, incubated with or without extract and with or without IL1β, was realized by qPCR with the StepOne, using SybRGreen staining reagent (Thermo Fisher Scientific).

    Article Title: Inflammatory and Humoral Immune Responses to Commercial Autogenous Salmonella Bacterin Vaccines in Light-Brown Leghorn Pullets: Primary and Secondary Vaccine Responses.
    Article Snippet: The quality and concentration of extracted total RNA were determined by NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. Total RNA was transcribed to cDNA using the High-Capacity cDNA kit with MultiScribe Reverse Transcriptase following manufacturer procedures (Thermo Fisher Scientific, Waltham, MA, USA). ..

    Article Title: Host metabolism balances microbial regulation of bile acid signalling.
    Article Snippet: Metabolites derived from the intestinal microbiota, including bile acids (BA), extensively modulate vertebrate physiology, including development, metabolism, immune responses and cognitive function.. However, to what extent host responses balance the physiological effects of microbiota-derived metabolites remains unclear.. Here, using untargeted metabolomics of mouse tissues, we identified a family of BA–methylcysteamine (BA–MCY) conjugates that are abundant in the intestine and dependent on vanin 1 (VNN1), a pantetheinase highly expressed in intestinal tissues.

    Article Title: Selection for Improved Water Efficiency in Broiler Breeder Lines Does Not Negatively Impact Immune Response Capabilities to Gram - and Gram + Bacterial Components and a Killed- Salmonella Enteritidis Vaccine.
    Article Snippet: The quality and concentration of total RNA were determined using a NanoDrop (Thermo Fisher Scientific, Waltham, MA, USA). .. A high-capacity cDNA kit with MultiScribe Reverse Transcriptase was used according to the manufacturer’s protocol (Thermo Fisher Scientific) to transcribe RNA into cDNA. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Potential Anti-Inflammatory and Chondroprotective Effect of Luzula sylvatica .
    Article Snippet: Total RNA was collected using Trizol (Invitrogen, Thermo Fisher Scientific) and treated with DNAse I (Invitrogen, Thermo Fisher Scientific) to remove genomic DNA. .. The Reverse transcriptase (RT) was then carried out with the MultiScribe reverse transcriptase (High Capacity cDNA Reverse Transcription Kit, Applied Biosystems, Thermo Fisher Scientific) using the StepOne apparatus (StepOne Real-Time PCR System, Applied Biosystems). .. The analysis of IL1β, MMP1, MMP2 and ADAMTS4 expression in the monolayers of NHAC-kn, incubated with or without extract and with or without IL1β, was realized by qPCR with the StepOne, using SybRGreen staining reagent (Thermo Fisher Scientific).

    other:

    Article Title: GLUT1 inhibitor BAY-876 induces apoptosis and enhances anti-cancer effects of bitter receptor agonists in head and neck squamous carcinoma cells
    Article Snippet: Between 200 – 500 ng of RNA were used to transcribe cDNA using MultiScribe Reverse Transcriptase (High-Capacity cDNA Kit; Thermo Fisher Scientific).


    Article Title: Enhancer-targeted CRISPR-Activation Rescues Haploinsufficient Autism Susceptibility Genes
    Article Snippet: RNA integrity and concentration was determined using an Agilent Bioanalyzer RNA Pico chip (Agilent). cDNA synthesis was performed using a Multiscribe High-Capacity cDNA synthesis kit (Thermo) or SuperScript IV (Thermo).

    Isolation:

    Article Title: Host metabolism balances microbial regulation of bile acid signalling.
    Article Snippet: Metabolites derived from the intestinal microbiota, including bile acids (BA), extensively modulate vertebrate physiology, including development, metabolism, immune responses and cognitive function.. However, to what extent host responses balance the physiological effects of microbiota-derived metabolites remains unclear.. Here, using untargeted metabolomics of mouse tissues, we identified a family of BA–methylcysteamine (BA–MCY) conjugates that are abundant in the intestine and dependent on vanin 1 (VNN1), a pantetheinase highly expressed in intestinal tissues.

    Synthesized:

    Article Title: Host metabolism balances microbial regulation of bile acid signalling.
    Article Snippet: Metabolites derived from the intestinal microbiota, including bile acids (BA), extensively modulate vertebrate physiology, including development, metabolism, immune responses and cognitive function.. However, to what extent host responses balance the physiological effects of microbiota-derived metabolites remains unclear.. Here, using untargeted metabolomics of mouse tissues, we identified a family of BA–methylcysteamine (BA–MCY) conjugates that are abundant in the intestine and dependent on vanin 1 (VNN1), a pantetheinase highly expressed in intestinal tissues.



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