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phusion high-fidelity dna polymerase  (New England Biolabs)


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    New England Biolabs phusion high-fidelity dna polymerase
    Phusion High Fidelity Dna Polymerase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 27744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pyrosequencing+method/Phusion+High-Fidelity+DNA+Polymerase/custom%40m0530%4028648779
    Average 99 stars, based on 27744 article reviews
    phusion high-fidelity dna polymerase - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Transduction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Purification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Nucleic Acid Purification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Magnetic Beads:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Inhibition:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Polyacrylamide Gel Electrophoresis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Cell Adhesion Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Centrifugation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Marker:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Immunoprecipitation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Next-Generation Sequencing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Immunohistochemistry:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    RNA Expression:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Polymerase Cycling Assembly:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Touchdown PCR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Sequencing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Dissection:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Over Expression:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Random Hexamer Labeling:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Avidin-Biotin Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Luciferase:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    In Vivo:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Filtration:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Expressing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Ancient DNA Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Subcloning:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Inverted Microscopy:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Introduce:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Transferring:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Infection:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA HS Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Viability Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Generated:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Plasmid Preparation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Size-exclusion Chromatography:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Chromatography:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Extraction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Binding Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Western Blot:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Synthesis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Mini Gene Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Negative Control:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Recombinant:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Ligand Binding Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Comparison:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Confocal Microscopy:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Formulation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    CRISPR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Blocking Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    GWAS:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    RNA Extraction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Modification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Synthesized:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Virus:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Neutralization:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Hybridization:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Purification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Transgenic Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Spectrophotometry:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Microarray:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Nucleic Acid Electrophoresis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Co-Culture Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Mutagenesis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    In Vitro:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Sample Prep:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Genotyping Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Protein Concentration:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Produced:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Fluorescence:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Extraction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Mass Spectrometry:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Clone Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Electroporation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Bicinchoninic Acid Protein Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Electrophoresis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    T7EI Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Derivative Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Stripping Membranes:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Ethanol Precipitation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Software:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    FLAG-tag:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Variant Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Suspension:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Northern Blot:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Staining:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Diagnostic Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Gel Purification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    FACS:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Real-time Polymerase Chain Reaction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Construct:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Cell Isolation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Disruption:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Cell Cycle Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Genomic Sequencing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    De-Phosphorylation Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Methylation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Sterility:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Molecular Cloning:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Gel Extraction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Nuclease Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Polymerase Chain Reaction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Nested PCR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Sequencing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    BrdU Incorporation Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Knock-Out:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Protease Inhibitor:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Labeling:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Sonication:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Quantitative RT-PCR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Flow Cytometry:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    PCR Cloning:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Labeling:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Gene Knockout:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Amplification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Ligation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Protein Extraction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Lambda DNA Preparation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    RNA Sequencing Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Cell Culture:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Incubation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Injection:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Evaporation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    PCR Splicing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Lysis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Activation Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Multiplex Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Transfection:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Imaging:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Electrophoretic Mobility Shift Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Emulsion:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    In Situ Hybridization:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Picogreen Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    In Silico:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Countercurrent Chromatography:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Formalin-fixed Paraffin-Embedded:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    cDNA Library Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Strep-tag:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Saline:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Oligonucleotide Synthesis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Sampling:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Diffusion-based Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Fractionation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Protein Purification:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Isolation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Selection:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Enzyme-linked Immunosorbent Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Agarose Gel Electrophoresis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Digital PCR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    High Throughput Screening Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Oligo Synthesis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Affinity Column:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Reporter Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Zinc-Fingers:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    shRNA:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Transformation Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Concentration Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Multiplexing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Chromatin Immunoprecipitation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Overlap Extension Polymerase Chain Reaction:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    SYBR Green Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Knock-In:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Reporter Gene Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Microscopy:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Functional Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Activity Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Patch Clamp:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Shotgun Sequencing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Nanopore Sequencing:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Gel Electrophoresis:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Hot Start PCR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Inverse PCR:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Positive Control:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Solubility:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Chromosome Walking:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Droplet Countercurrent Chromatography:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    BAC Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Fluorescence In Situ Hybridization:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Stable Transfection:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Immunofluorescence:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    TA Cloning:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Quantitation Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Cell Viability Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Non-Homologous End Joining:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Translocation Assay:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Bacteria:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    DNA Ligation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Electron Microscopy:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Hi-C:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur

    Encapsulation:

    Article Title: DNA methylation regulates the secondary metabolism of saponins to improve the adaptability of Eleutherococcus senticosus during drought stress
    Article Snippet: system (Beckman Coulter, Beverly, USA) was used to purify the library fragments to select cDNA fragments that were preferably 250–300 bp in length. Then, size-selected, adaptor-ligated cDNA was treated with 3 µL USER Enzyme (NEB, USA) at 37°C for 15 min and then heated to 95°C for 5 min before PCR. Next, Phusion high-fidelity DNA polymerase, universal PCR primers, and index (X) primers were used for PCR. Finally, PCR products were purified (AMPur



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    Single Tube Multiplex Pyrosequencing Method, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multiplex+pyrosequencing+method/single+tube+multiplex+pyrosequencing+assay/pm25069408-51-3-5
    Average 90 stars, based on 1 article reviews
    single-tube multiplex pyrosequencing method - by Bioz Stars, 2026-10
    90/100 stars
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