Journal: bioRxiv
Article Title: Transplantation of Human IPSC-derived Microglia Ameliorates Neuropathology and Circuit Dysfunction in Progranulin-Deficient Mice
doi: 10.64898/2026.01.13.699312
Figure Lengend Snippet: (A) Representative image of multi-unit recordings setup of ex vivo thalamic horizontal slices in a humidified, oxygenated interface chamber. (B) Schematic of multi-unit activity recordings with a Neuronexus 16-channel recording electrode positioned in the thalamus following electrical stimulation of the internal capsule. (C) Representative 3-second recording of intrathalamic multi-unit activity evoked by stimulation of the internal capsule (black dot indicates time of stimulation). Only 12 of 16 channels are shown for clarity. (D) Collapsed activity from 12 recording channels in (C). Black dot indicates time of stimulation. (E) Post-stimulus time histogram of instantaneous spiking frequency from multi-unit activity recorded across 16 channels (all 16 channels were analyzed, 12 representative channels are displayed in (C) from 15 slices from 9 hCSF1 (WT) mice, 8 slices from 7 hCSF1-Grn −/− +KOxMG mice, and 12 slices from 9 hCSF1-Grn −/− +WTxMG mice. The grey box denotes early response (0–1.5 s), and the white box denotes delayed response (1.5–3 s) after stimulation. (F) Enlarged instantaneous frequency of the delayed response (1.5–3 s) from (D). (G) Relative probability of eliciting spiking during the direct response (0–1.5 s) after stimulation; p values from Kolmogorov-Smirnov test with Bonferroni correction (* p = 0.0024). (H) Instantaneous frequency of spiking during the early response (0–1.5 s) after stimulation; Kruskal–Wallis test, H(3) = 6.767, p = 0.0797. (I) Relative probability of eliciting spiking during the delayed response (1.5–3 s) after stimulation; p values from Kolmogorov-Smirnov test with Bonferroni correction (**** p < 0.0001). (J) Instantaneous frequency of spiking during the delayed response (1.5–3 s) after stimulation; Kruskal-Wallis test, H(3)=56.6, **** p < 0.0001, and Dunn’s multiple comparisons test (**** p < 0.0001). Data are presented as mean ± SEM. Comparisons not shown are not significant.
Article Snippet: Extracellular multi-unit activity (MUA) recordings were obtained with a linear 16-channel multi-electrode array (Neuronexus) that spanned the nRT and VB thalamic regions.
Techniques: Ex Vivo, Activity Assay
NeuroNexus Technologies is a verified supplier 
NeuroNexus Technologies manufactures this product 
