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rnasefree dnase set  (Qiagen)


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    Structured Review

    Qiagen rnasefree dnase set
    Rnasefree Dnase Set, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 26814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multi-analyte+elisarray+kit/RNase-Free+DNase+Set/ppr0237148-48-11-25
    Average 99 stars, based on 26814 article reviews
    rnasefree dnase set - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Spectrophotometry:

    Article Title: Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1A
    Article Snippet: RNA was extracted from mouse lumbar roots, sciatic nerves, and femoral nerves using the Qiagen RNeasy Lipid Tissue Mini Kit (Qiagen, 74804) following the manufacturer’s protocol from snap-frozen tissues. .. After DNase treatment (Qiagen, 79254), RNA was quantified by spectrophotometry, and 0.3 μg of RNA was used to synthesize cDNA using TaqMan reverse-transcription reagents (Applied Biosystems, N8080234). .. Expression of hu PMP22 (Hs00991884_m1), mu Pmp22 (Mm01333393_m1), and mu Mpz (Mm00485141_g1) mRNA were quantified using TaqMan gene expression assays (Applied Biosystems) and mu Gapdh assay (Mm99999915_g1) as an endogenous control.

    Reverse Transcription:

    Article Title: Safety, efficacy, and distal nerve Schwann cell biodistribution in mice and NHPs to support translation of AAV9 RNAi therapy for CMT1A
    Article Snippet: RNA was extracted from mouse lumbar roots, sciatic nerves, and femoral nerves using the Qiagen RNeasy Lipid Tissue Mini Kit (Qiagen, 74804) following the manufacturer’s protocol from snap-frozen tissues. .. After DNase treatment (Qiagen, 79254), RNA was quantified by spectrophotometry, and 0.3 μg of RNA was used to synthesize cDNA using TaqMan reverse-transcription reagents (Applied Biosystems, N8080234). .. Expression of hu PMP22 (Hs00991884_m1), mu Pmp22 (Mm01333393_m1), and mu Mpz (Mm00485141_g1) mRNA were quantified using TaqMan gene expression assays (Applied Biosystems) and mu Gapdh assay (Mm99999915_g1) as an endogenous control.

    Isolation:

    Article Title: AID and TET2 cooperate to demethylate Irf4 for plasma cell fate in germinal center B cells
    Article Snippet: Total RNA was purified from sorted cells using RNeasy kit #74104 (Qiagen). .. Each RNA sample was treated with an RNase-free DNase kit #79254 (Qiagen) during the RNA isolation procedure. .. The concentration of purified total RNA was measured by NanoDrop 2000 (Thermo Fisher Scientific).

    Article Title: Exploring Resistance to ETS Targeting Agents in Diffuse Large B‐Cell Lymphoma
    Article Snippet: Total RNA was extracted using TRIzol reagent (Invitrogen) according to the manufacturer's protocol. .. During RNA isolation, samples were treated with RNase‐free DNase (Qiagen, Hilden, Germany) to remove potential genomic DNA contamination. .. RNA concentration was measured using a Nanodrop spectrometer (DS‐11 spectrophotometer, DeNovix) at 260 nm.

    Real-time Polymerase Chain Reaction:

    Article Title: Generation of spinal cord organoids from human induced pluripotent stem cells caudalised to a lumbar fate.
    Article Snippet: Neurite length was analyzed by one-way ANOVA with multiple comparisons test using Prism 10.0 (Graphpad). .. Real-time qPCR for spinal cord region specification Five day 40 organoids were pooled per sample from each differentiation (3 independent differentiations) and RNA was extracted using the RNeasy Mini kit (Qiagen 74104) and RNaseFree DNase set (QIAGEN 79254) as per manufacturer’s protocol. .. For RT-PCR, cDNA was reverse transcribed from 500ng of RNA using the iScript cDNA Synthesis Kit (Bio-Rad 1708891). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) on a CFX96 machine (Bio-Rad).

    Purification:

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: 6.11.4 Viral RNA Extraction and Reverse Transcription Quantitative PCR Viral RNA was extracted by Viral RNA Mini Kit (QIAGEN, Cat #52906, USA) according to the manufacturer's instructions. .. Extracted RNA were treated with DNase I (QIAGEN, Cat #79254, USA) endonuclease according to the manufacturer's protocol and purified by RNeasy Mini Kit (QIAGEN, Cat #74106, USA) for RNA Purification to exclude plasmid DNA contamination. ..

    Plasmid Preparation:

    Article Title: Nucleic acid molecules and dual-functional peptides having antiviral activity and delivery activity, compositions and methods thereof
    Article Snippet: 6.11.4 Viral RNA Extraction and Reverse Transcription Quantitative PCR Viral RNA was extracted by Viral RNA Mini Kit (QIAGEN, Cat #52906, USA) according to the manufacturer's instructions. .. Extracted RNA were treated with DNase I (QIAGEN, Cat #79254, USA) endonuclease according to the manufacturer's protocol and purified by RNeasy Mini Kit (QIAGEN, Cat #74106, USA) for RNA Purification to exclude plasmid DNA contamination. ..



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    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory <t>Cytokine</t> <t>ELISArray</t> Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.
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    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

    Journal: Cells

    Article Title: Signalling Pathways of Inflammation and Cancer in Human Mononuclear Cells: Effect of Nanoparticle Air Pollutants

    doi: 10.3390/cells13161367

    Figure Lengend Snippet: The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

    Article Snippet: TNFα was quantified in the culture medium of M and MDM with the Multi-Analyte Inflammatory Cytokine ELISArray Kit (Qiagen, Manchester, UK).

    Techniques: Membrane, Derivative Assay, Incubation, CyQUANT Assay, LDH Cytotoxicity Assay, Staining, Fluorescence, Flow Cytometry, Control