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aav hsyn flex mgfp 2a synaptophysin mruby  (Addgene inc)


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    Addgene inc aav hsyn flex mgfp 2a synaptophysin mruby
    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST <t>neurons.</t> <t>The</t> <t>AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby</t> was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
    Aav Hsyn Flex Mgfp 2a Synaptophysin Mruby, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mruby/pAAV+hSyn+FLEx+mGFP-2A-Synaptophysin-mRuby+(Plasmid+%2371760)/pmc12940476-140-9-12
    Average 94 stars, based on 63 article reviews
    aav hsyn flex mgfp 2a synaptophysin mruby - by Bioz Stars, 2026-09
    94/100 stars

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    1) Product Images from "Central neurons encode interleukin-1β signals and mediate stress-induced inflammation"

    Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation

    Journal: The Journal of Experimental Medicine

    doi: 10.1084/jem.20252000

    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.
    Figure Legend Snippet: BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Techniques Used: Anterograde Tracing, Injection, Expressing, Saline, Control

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    Labeling:

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    Article Snippet: .. For specific axon labeling of the mPFC Foxp2 + neurons, pAAV1-hSyn-DIO-mGFP-Synaptophysin-mRuby (Addgene, Cat# 71760) was unilaterally injected into the mPFC of Foxp2 -Cre mice. .. For monosynaptic retrograde rabies tracing, a 150 nl mixture of AAV helpers (rAAV2/9-EF1a-DIO-mCherry-TVA and rAAV2/9-EF1a-DIO-RVG) (BrainVTA, Cat# PT0023 and PT0207) was unilaterally injected into the mPFC of the Foxp2 -Cre mice.

    Article Title: A molecularly defined basalo-prefrontal-thalamic circuit regulates sensory and affective dimensions of pain in male mice.
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    Injection:

    Article Title: A molecularly defined basalo-prefrontal-thalamic circuit regulates sensory and affective dimensions of pain in male mice
    Article Snippet: .. For specific axon labeling of the mPFC Foxp2 + neurons, pAAV1-hSyn-DIO-mGFP-Synaptophysin-mRuby (Addgene, Cat# 71760) was unilaterally injected into the mPFC of Foxp2 -Cre mice. .. For monosynaptic retrograde rabies tracing, a 150 nl mixture of AAV helpers (rAAV2/9-EF1a-DIO-mCherry-TVA and rAAV2/9-EF1a-DIO-RVG) (BrainVTA, Cat# PT0023 and PT0207) was unilaterally injected into the mPFC of the Foxp2 -Cre mice.

    Article Title: A molecularly defined basalo-prefrontal-thalamic circuit regulates sensory and affective dimensions of pain in male mice.
    Article Snippet: .. For specific axon labeling of the mPFC Foxp2+ neurons, pAAV1-hSyn-DIO-mGFPSynaptophysin-mRuby (Addgene, Cat# 71760) was unilaterally injected into the mPFC of Foxp2-Cre mice. .. For monosynaptic retrograde rabies tracing, a 150nl mixture of AAV helpers (rAAV2/9-EF1a-DIO-mCherry-TVA and rAAV2/9-EF1a-DIORVG) (BrainVTA, Cat# PT0023 and PT0207) was unilaterally injected into the mPFC of the Foxp2-Cre mice.

    Bioprocessing:

    Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation
    Article Snippet: For the ablation studies, either AAV-EF1a-mCherry-flex-dtA (cat # NRZP-0622-ZP616; Creative Biolabs), or AAV-hSyn-DIO-EGFP (cat #50457; Addgene) was utilized. .. For the tracing studies, either AAV-hSyn-DIO-EGFP (cat #50457; Addgene), AAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (cat# 71760; Addgene), AAV-Ef1a-fDIO-EYFP (cat# 55641; Addgene), or AAV pEF1a-DIO-FLPo-WPRE-hGHpA (cat# 87306; Addgene) was utilized. ..

    Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation.
    Article Snippet: For the ablation studies, either AAV-EF1a-mCherry-flex-dtA (cat # NRZP-0622-ZP616; Creative Biolabs), or AAV-hSyn-DIO-EGFP (cat #50457; Addgene) was utilized. .. For the tracing studies, either AAV-hSyn-DIO-EGFP (cat #50457; Addgene), AAV-hSynFLEx-mGFP-2A-Synaptophysin-mRuby (cat# 71760; Addgene), AAV-Ef1a-fDIO-EYFP (cat# 55641; Addgene), or AAV pEF1a-DIOFLPo-WPRE-hGHpA (cat# 87306; Addgene) was utilized. ..

    other:

    Article Title: Distinct modes of dopamine modulation on striatopallidal synaptic transmission.
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    Article Title: Brain-wide mapping of developmental trajectories of cerebellar efferent projections
    Article Snippet: To anterogradely trace cerebellar long-range projections, we utilized an EGFP lentivirus (LV-H1p-UbCpEGFP) in wild-type mice and Cre-dependent viral vectors pAAV CAG-FLEX-EGFP-WPRE (Addgene #51502, AAV1) or pAAV hSyn.FLEX.mGFP.2A.Synaptophysin-mRuby (Addgene #71760, AAV1) in Ntsr1 Cre and Vgat1 Cre mice.

    Produced:

    Article Title: Interhemispheric CA1 projections to the subiculum support spatial cognition and are affected in a mouse model of the 22q11.2 deletion syndrome.
    Article Snippet: .. All animal procedures were approved by the CSIC, the Community of Madrid Ethics Committees on Animal Experimentation (PROEX 123-17 and 124-17) and the Generalitat Valenciana REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP antibody produced in chicken AVES Labs #GFP-1020 RRID:AB_10000240 Goat anti-Chicken IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11039 RRID:AB_142924 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379 RRID:AB_2209751 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 Thermo-Fisher Scientific #A11036 RRID:AB_10563566 Anti-GFP polyclonal antibody produced in rabbit Thermo-Fisher Scientific #A11122 RRID:AB_221569 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11034 RRID:AB_2576217 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 488 Conjugate Thermo-Fisher Scientific #C22841 Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 555 Conjugate Thermo-Fisher Scientific #C34776 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 Tg(Lypd1-Cre)NR151Gsat The Gene Expression Nervous System Atlas (GENSAT) Project57 MGI:5435531 Df16(A) Mus musculus Joseph Gogos95 N/A Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930/Addgene #71760 AAV2/9 CAG-FLEX-eGFP-WPRE Addgene #51502 AAV2/1 hSyn1-DIO-eOPN3-mScarlet-WPRE Ofer Yizhar,65 Cristina Fregola Modified from Addgene #125713 pCAG-GFP Addgene #11150 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Cell Reports 45, 117114, April 28, 2026 15 Agriculture Department, in compliance with national and European legislation (2020/VSC/PEA/0227). ..

    Recombinant:

    Article Title: Interhemispheric CA1 projections to the subiculum support spatial cognition and are affected in a mouse model of the 22q11.2 deletion syndrome.
    Article Snippet: .. All animal procedures were approved by the CSIC, the Community of Madrid Ethics Committees on Animal Experimentation (PROEX 123-17 and 124-17) and the Generalitat Valenciana REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP antibody produced in chicken AVES Labs #GFP-1020 RRID:AB_10000240 Goat anti-Chicken IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11039 RRID:AB_142924 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379 RRID:AB_2209751 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 Thermo-Fisher Scientific #A11036 RRID:AB_10563566 Anti-GFP polyclonal antibody produced in rabbit Thermo-Fisher Scientific #A11122 RRID:AB_221569 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11034 RRID:AB_2576217 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 488 Conjugate Thermo-Fisher Scientific #C22841 Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 555 Conjugate Thermo-Fisher Scientific #C34776 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 Tg(Lypd1-Cre)NR151Gsat The Gene Expression Nervous System Atlas (GENSAT) Project57 MGI:5435531 Df16(A) Mus musculus Joseph Gogos95 N/A Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930/Addgene #71760 AAV2/9 CAG-FLEX-eGFP-WPRE Addgene #51502 AAV2/1 hSyn1-DIO-eOPN3-mScarlet-WPRE Ofer Yizhar,65 Cristina Fregola Modified from Addgene #125713 pCAG-GFP Addgene #11150 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Cell Reports 45, 117114, April 28, 2026 15 Agriculture Department, in compliance with national and European legislation (2020/VSC/PEA/0227). ..

    Gene Expression:

    Article Title: Interhemispheric CA1 projections to the subiculum support spatial cognition and are affected in a mouse model of the 22q11.2 deletion syndrome.
    Article Snippet: .. All animal procedures were approved by the CSIC, the Community of Madrid Ethics Committees on Animal Experimentation (PROEX 123-17 and 124-17) and the Generalitat Valenciana REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP antibody produced in chicken AVES Labs #GFP-1020 RRID:AB_10000240 Goat anti-Chicken IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11039 RRID:AB_142924 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379 RRID:AB_2209751 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 Thermo-Fisher Scientific #A11036 RRID:AB_10563566 Anti-GFP polyclonal antibody produced in rabbit Thermo-Fisher Scientific #A11122 RRID:AB_221569 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11034 RRID:AB_2576217 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 488 Conjugate Thermo-Fisher Scientific #C22841 Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 555 Conjugate Thermo-Fisher Scientific #C34776 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 Tg(Lypd1-Cre)NR151Gsat The Gene Expression Nervous System Atlas (GENSAT) Project57 MGI:5435531 Df16(A) Mus musculus Joseph Gogos95 N/A Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930/Addgene #71760 AAV2/9 CAG-FLEX-eGFP-WPRE Addgene #51502 AAV2/1 hSyn1-DIO-eOPN3-mScarlet-WPRE Ofer Yizhar,65 Cristina Fregola Modified from Addgene #125713 pCAG-GFP Addgene #11150 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Cell Reports 45, 117114, April 28, 2026 15 Agriculture Department, in compliance with national and European legislation (2020/VSC/PEA/0227). ..

    Modification:

    Article Title: Interhemispheric CA1 projections to the subiculum support spatial cognition and are affected in a mouse model of the 22q11.2 deletion syndrome.
    Article Snippet: .. All animal procedures were approved by the CSIC, the Community of Madrid Ethics Committees on Animal Experimentation (PROEX 123-17 and 124-17) and the Generalitat Valenciana REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP antibody produced in chicken AVES Labs #GFP-1020 RRID:AB_10000240 Goat anti-Chicken IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11039 RRID:AB_142924 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379 RRID:AB_2209751 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 Thermo-Fisher Scientific #A11036 RRID:AB_10563566 Anti-GFP polyclonal antibody produced in rabbit Thermo-Fisher Scientific #A11122 RRID:AB_221569 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11034 RRID:AB_2576217 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 488 Conjugate Thermo-Fisher Scientific #C22841 Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 555 Conjugate Thermo-Fisher Scientific #C34776 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 Tg(Lypd1-Cre)NR151Gsat The Gene Expression Nervous System Atlas (GENSAT) Project57 MGI:5435531 Df16(A) Mus musculus Joseph Gogos95 N/A Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930/Addgene #71760 AAV2/9 CAG-FLEX-eGFP-WPRE Addgene #51502 AAV2/1 hSyn1-DIO-eOPN3-mScarlet-WPRE Ofer Yizhar,65 Cristina Fregola Modified from Addgene #125713 pCAG-GFP Addgene #11150 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Cell Reports 45, 117114, April 28, 2026 15 Agriculture Department, in compliance with national and European legislation (2020/VSC/PEA/0227). ..

    Software:

    Article Title: Interhemispheric CA1 projections to the subiculum support spatial cognition and are affected in a mouse model of the 22q11.2 deletion syndrome.
    Article Snippet: .. All animal procedures were approved by the CSIC, the Community of Madrid Ethics Committees on Animal Experimentation (PROEX 123-17 and 124-17) and the Generalitat Valenciana REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-GFP antibody produced in chicken AVES Labs #GFP-1020 RRID:AB_10000240 Goat anti-Chicken IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11039 RRID:AB_142924 Anti-RFP antibody produced in rabbit Rockland Antibody #600-401-379 RRID:AB_2209751 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 568 Thermo-Fisher Scientific #A11036 RRID:AB_10563566 Anti-GFP polyclonal antibody produced in rabbit Thermo-Fisher Scientific #A11122 RRID:AB_221569 Goat anti-Rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa FluorTM 488 Thermo-Fisher Scientific #A11034 RRID:AB_2576217 Chemicals, peptides, and recombinant proteins Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 488 Conjugate Thermo-Fisher Scientific #C22841 Cholera Toxin Subunit B (Recombinant), Alexa FluorTM 555 Conjugate Thermo-Fisher Scientific #C34776 Experimental models: Organisms/strains C57BL/6J Mus musculus Jackson Laboratories RRID:IMSR_JAX:000664 Tg(Lypd1-Cre)NR151Gsat The Gene Expression Nervous System Atlas (GENSAT) Project57 MGI:5435531 Df16(A) Mus musculus Joseph Gogos95 N/A Recombinant DNA AAV2/DJ hSyn.FLEX.mGFP.2A.Synatophysin-mRuby Stanford vector core #1930/Addgene #71760 AAV2/9 CAG-FLEX-eGFP-WPRE Addgene #51502 AAV2/1 hSyn1-DIO-eOPN3-mScarlet-WPRE Ofer Yizhar,65 Cristina Fregola Modified from Addgene #125713 pCAG-GFP Addgene #11150 Software AxoGraph AxoGraph 1.6.4 PRISM 9 Graphpad 9.0.1 (128) Microsoft Office Word Microsoft 2019 16.56 Microsoft Office Exel Microsoft 2019 Adobe Illustrator Adobe 2020 v24.1 FIDJI GPL v2 2.3.0/1.53f Leica Application Suite X Leica v3.7.4 ANY-maze Stoelting Co. 4.99 Cell Reports 45, 117114, April 28, 2026 15 Agriculture Department, in compliance with national and European legislation (2020/VSC/PEA/0227). ..



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    BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Journal: The Journal of Experimental Medicine

    Article Title: Central neurons encode interleukin-1β signals and mediate stress-induced inflammation

    doi: 10.1084/jem.20252000

    Figure Lengend Snippet: BNST–PVN-RVLM neural signaling mediates IL-1β–induced changes in heart rate and IL-6. (A) Schematic for anterograde tracing of axonal projections and terminals from PBS-TRAPed or IL-1β–TRAPed BNST neurons. The AAV-hSyn-FLEx-mGFP-2A-synaptophysin-mRuby was injected into the BNST of TRAP2 mice. (B) Representative image for GFP + axons and mRuby + terminals in the PVN of PBS-TRAPed or IL-1β–TRAPed mice. The rightmost panel shows a higher-magnification view of the PVN in IL-1β–TRAPed mice. Arrowheads show regions with co-localization of mRuby and EYFP. Scale bars, 100 μm. (C) Schematic for anterograde tracing of IL-1β–TRAPed BNST neurons connected with PVN neurons. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-Ef1a-fDIO-EYFP was injected into the PVN of TRAP2 mice. (D) Representative image for EYFP expression in the PVN, RVLM, and NTS. Arrowheads show neurons, and arrows show axonal projections with expression of EYFP. Scale bar, 100 μm for the PVN and RVLM. Scale bar, 200 μm for the NTS. (E) c-Fos expression in the RVLM after reactivation with saline as a control or CNO of IL-1β–responsive BNST neurons. Scale bar, 100 μm. (F) Schematic for activating the BNST–PVN neural pathway. The AAV-pEF1a-DIO-FLPo-WPRE-hGHpA was injected into the BNST, and the AAV-hSyn-fDIO-hM3D(Gq)-mCherry-WPREpA was injected into the PVN of TRAP2 mice. (G) Representative image of PVN showing Gq-DREADD-mCherry–expressing cells (red). Scale bar, 100 μm. (H) Serum IL-6 levels at 2 h after reactivation with saline as a control or CNO of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (I) ΔHR for 60 min after reactivation of the BNST–PVN neuronal pathway: saline (black) or CNO (red) (saline, n = 7 mice; CNO, n = 10 mice, mixed-effects analysis with Šidák correction). (J) AUC of ΔHR after reactivation. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. (K) Serum corticosterone levels at 2 h after reactivation of the BNST–PVN neuronal pathway. Data are represented as individual mouse data points pooled from two independent experiments. Unpaired t test. *P < 0.05; **P < 0.01.

    Article Snippet: For the tracing studies, either AAV-hSyn-DIO-EGFP (cat #50457; Addgene), AAV-hSyn-FLEx-mGFP-2A-Synaptophysin-mRuby (cat# 71760; Addgene), AAV-Ef1a-fDIO-EYFP (cat# 55641; Addgene), or AAV pEF1a-DIO-FLPo-WPRE-hGHpA (cat# 87306; Addgene) was utilized.

    Techniques: Anterograde Tracing, Injection, Expressing, Saline, Control