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hepg2 mrna microarray  (Arraystar inc)

 
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    Arraystar inc hepg2 mrna microarray
    Suppression of LRH-1 in <t>HepG2</t> prevents its growth and colony formation capacity in vitro. Notes: ( A ) Schematic diagram of the TALENs targeting the second exon of LRH-1 and Western blotting (WB) validation of HepG2 LRH-1/− cell clones. ( B ) Proliferation assay by MTT. Two HepG2 LRH-1/− cell clones proliferated at slower rates than the parental HepG2 cells. ( C ) Colony formation assay. Results showed that HepG2 LRH-1/− cells formed smaller and less colonies than parental HepG2 cells. ** P <0.01 vs parental HepG2 cells. Abbreviation: TALENs, transcription activator-like effector nucleases.
    Hepg2 Mrna Microarray, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mrna+microarray/hepg2+mrna+microarray/pmc06078084-72-0-6
    Average 90 stars, based on 1 article reviews
    hepg2 mrna microarray - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "LRH-1 drives hepatocellular carcinoma partially through induction of c-myc and cyclin E1, and suppression of p21"

    Article Title: LRH-1 drives hepatocellular carcinoma partially through induction of c-myc and cyclin E1, and suppression of p21

    Journal: Cancer Management and Research

    doi: 10.2147/CMAR.S162887

    Suppression of LRH-1 in HepG2 prevents its growth and colony formation capacity in vitro. Notes: ( A ) Schematic diagram of the TALENs targeting the second exon of LRH-1 and Western blotting (WB) validation of HepG2 LRH-1/− cell clones. ( B ) Proliferation assay by MTT. Two HepG2 LRH-1/− cell clones proliferated at slower rates than the parental HepG2 cells. ( C ) Colony formation assay. Results showed that HepG2 LRH-1/− cells formed smaller and less colonies than parental HepG2 cells. ** P <0.01 vs parental HepG2 cells. Abbreviation: TALENs, transcription activator-like effector nucleases.
    Figure Legend Snippet: Suppression of LRH-1 in HepG2 prevents its growth and colony formation capacity in vitro. Notes: ( A ) Schematic diagram of the TALENs targeting the second exon of LRH-1 and Western blotting (WB) validation of HepG2 LRH-1/− cell clones. ( B ) Proliferation assay by MTT. Two HepG2 LRH-1/− cell clones proliferated at slower rates than the parental HepG2 cells. ( C ) Colony formation assay. Results showed that HepG2 LRH-1/− cells formed smaller and less colonies than parental HepG2 cells. ** P <0.01 vs parental HepG2 cells. Abbreviation: TALENs, transcription activator-like effector nucleases.

    Techniques Used: In Vitro, TALENs, Western Blot, Biomarker Discovery, Clone Assay, Proliferation Assay, Colony Assay

    Suppression of LRH-1 in HepG2 attenuates its growth in vivo. Notes: ( A and B ) Growth curve shows the growth patterns of tumors formed by HepG2 LRH-1/− cell clones and parental HepG2 cells, HepG2 cells formed xenograft tumors with larger sizes and at faster rates as compared to HepG2 LRH-1/− #1 cells, while HepG2 LRH-1/− #2 lost the in vivo tumorigenic capacity in all nude mice (NT, no tumor). ( C ) Ki67 immunohistochemistry. Our results suggested that significantly lower and less Ki67 immunoreactivity was seen in xenograft tumors formed from HepG2 LRH-1/− than parental HepG2 cells. Scale bar =100 μm.** P <0.01 vs parental HepG2 cells.
    Figure Legend Snippet: Suppression of LRH-1 in HepG2 attenuates its growth in vivo. Notes: ( A and B ) Growth curve shows the growth patterns of tumors formed by HepG2 LRH-1/− cell clones and parental HepG2 cells, HepG2 cells formed xenograft tumors with larger sizes and at faster rates as compared to HepG2 LRH-1/− #1 cells, while HepG2 LRH-1/− #2 lost the in vivo tumorigenic capacity in all nude mice (NT, no tumor). ( C ) Ki67 immunohistochemistry. Our results suggested that significantly lower and less Ki67 immunoreactivity was seen in xenograft tumors formed from HepG2 LRH-1/− than parental HepG2 cells. Scale bar =100 μm.** P <0.01 vs parental HepG2 cells.

    Techniques Used: In Vivo, Clone Assay, Immunohistochemistry

    Gene expression microarray analysis for the identification of LRH-1-regulated genes in HepG2 cells. Notes: ( A ) Gene ontology (GO) analysis of genes that are LRH-1-regulated in HepG2 and in HepG2 LRH-1/− cells. ( B ) KEGG pathway enrichment analysis for LRH-1-regulated genes in HepG2 cells are shown. ( C ) Several cell cycle-related genes that altered after suppression of LRH-1 were listed. Abbreviations: BP, biological process; DE, differential expression; KEGG, Kyoto Encyclopedia of Genes and Genomes.
    Figure Legend Snippet: Gene expression microarray analysis for the identification of LRH-1-regulated genes in HepG2 cells. Notes: ( A ) Gene ontology (GO) analysis of genes that are LRH-1-regulated in HepG2 and in HepG2 LRH-1/− cells. ( B ) KEGG pathway enrichment analysis for LRH-1-regulated genes in HepG2 cells are shown. ( C ) Several cell cycle-related genes that altered after suppression of LRH-1 were listed. Abbreviations: BP, biological process; DE, differential expression; KEGG, Kyoto Encyclopedia of Genes and Genomes.

    Techniques Used: Gene Expression, Microarray, Quantitative Proteomics

    LRH-1 promotes the development and progression of HCC through induction of c-myc and cyclin E1, and suppression of p21. Notes: ( A ) qRT-PCR analysis of c-myc, cyclin E1, and p21 genes in HepG2 and HuH-7 cells with ectopic LRH-1 expression. Our data indicated that ectopic expression of LRH-1 dramatically induced the mRNA and protein levels of c-myc and cyclin E1, and attenuated, in the meanwhile, the expression of p21. ( B ) Western blotting validation of c-myc, cyclin E1, and p21 in HepG2 and HuH-7 with ectopic LRH-1 expression. ( C ) Reporter assay showed that luciferase activity of c-myc and cyclin E1 promoter-luc reporter was significantly enhanced, while that of p21 was dramatically suppressed, in HepG2-LRH-1 cells. ( D ) MTT results indicated that the increased growth ability of HepG2-LRH-1 cells could be, at least partially, reversed by suppressing the expression of c-myc or restoring the expression of p21. ( E ) Schematic diagram showing the hypothesized pathways of LRH-1-mediated cell growth in HCC via its transcriptional repression of p21 and transactivation of c-myc and cyclin E1. * P <0.05; ** P <0.01; # not significant. Abbreviations: HCC, hepatocellular carcinoma; luc, luciferase.
    Figure Legend Snippet: LRH-1 promotes the development and progression of HCC through induction of c-myc and cyclin E1, and suppression of p21. Notes: ( A ) qRT-PCR analysis of c-myc, cyclin E1, and p21 genes in HepG2 and HuH-7 cells with ectopic LRH-1 expression. Our data indicated that ectopic expression of LRH-1 dramatically induced the mRNA and protein levels of c-myc and cyclin E1, and attenuated, in the meanwhile, the expression of p21. ( B ) Western blotting validation of c-myc, cyclin E1, and p21 in HepG2 and HuH-7 with ectopic LRH-1 expression. ( C ) Reporter assay showed that luciferase activity of c-myc and cyclin E1 promoter-luc reporter was significantly enhanced, while that of p21 was dramatically suppressed, in HepG2-LRH-1 cells. ( D ) MTT results indicated that the increased growth ability of HepG2-LRH-1 cells could be, at least partially, reversed by suppressing the expression of c-myc or restoring the expression of p21. ( E ) Schematic diagram showing the hypothesized pathways of LRH-1-mediated cell growth in HCC via its transcriptional repression of p21 and transactivation of c-myc and cyclin E1. * P <0.05; ** P <0.01; # not significant. Abbreviations: HCC, hepatocellular carcinoma; luc, luciferase.

    Techniques Used: Quantitative RT-PCR, Expressing, Western Blot, Biomarker Discovery, Reporter Assay, Luciferase, Activity Assay

    Related Articles

    Microarray:

    Article Title: LRH-1 drives hepatocellular carcinoma partially through induction of c-myc and cyclin E1, and suppression of p21
    Article Snippet: Cell lysates were collected and applied for LRH-1, p21, cyclin E1, c-myc, and β-actin detection by using mouse monoclonal LRH-1 antibody (Catalog: ab41901, Abcam, Cambridge, UK), rabbit polyclonal c-myc antibody (Catalog: sc-788, Santa Cruz Biotechnology Inc., Dallas, TX, USA), rabbit polyclonal cyclin E1 antibody (Catalog: sc-198, Santa Cruz Biotechnology Inc.), and rabbit monoclonal p21 antibody (Catalog: 2947, Cell Signaling Technology, Danvers, MA, USA). .. HepG2 mRNA microarray was performed with Arraystar Human LncRNA Microarray v4.0 according to the manufacturer’s protocol. ..



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    Image Search Results


    m6A mRNA Epitranscriptomic Microarray analysis. A-C : Hierarchical clustering heatmap of EMCI/Control ( A ), LMCI/Control ( B ), and LMCI/EMCI ( C ). Each row represented one mRNA, and each column represented one sample. D-F : Volcano plot analysis of EMCI/Control ( D ), LMCI/Control ( E ), and LMCI/EMCI ( F ). Red boxes represented upregulated transcripts (≥ 1.5-fold change, P < 0.05), and blue boxes represented downregulated transcripts (≤ 0.7-fold change, P < 0.05). G-I : Four-quadrant diagram of changed transcripts in both m6A methylation and mRNA expression of EMCI/Control ( G ), LMCI/Control ( H ), and LMCI/EMCI ( I ). Red dots: hypermethylated-upregulated mRNAs; green dots: hypermethylated-downregulated mRNAs; purple dots: hypomethylated-upregulated mRNAs; blue dots: hypomethylated-downregulated mRNAs. P < 0.05. J-L : The interaction between m6A methylation and mRNA expression. ( J ) Hypermethylation-upregulation, ( K ) Hypomethylation-upregulation, ( L ) Hypomethylation-downregulation

    Journal: Alzheimer's Research & Therapy

    Article Title: Linking oxysterols and different stages of mild cognitive impairment: insights from gut metabolites and N6-methyladenosine

    doi: 10.1186/s13195-025-01743-5

    Figure Lengend Snippet: m6A mRNA Epitranscriptomic Microarray analysis. A-C : Hierarchical clustering heatmap of EMCI/Control ( A ), LMCI/Control ( B ), and LMCI/EMCI ( C ). Each row represented one mRNA, and each column represented one sample. D-F : Volcano plot analysis of EMCI/Control ( D ), LMCI/Control ( E ), and LMCI/EMCI ( F ). Red boxes represented upregulated transcripts (≥ 1.5-fold change, P < 0.05), and blue boxes represented downregulated transcripts (≤ 0.7-fold change, P < 0.05). G-I : Four-quadrant diagram of changed transcripts in both m6A methylation and mRNA expression of EMCI/Control ( G ), LMCI/Control ( H ), and LMCI/EMCI ( I ). Red dots: hypermethylated-upregulated mRNAs; green dots: hypermethylated-downregulated mRNAs; purple dots: hypomethylated-upregulated mRNAs; blue dots: hypomethylated-downregulated mRNAs. P < 0.05. J-L : The interaction between m6A methylation and mRNA expression. ( J ) Hypermethylation-upregulation, ( K ) Hypomethylation-upregulation, ( L ) Hypomethylation-downregulation

    Article Snippet: The cRNAs were combined and hybridized onto Arraystar Human mRNA Epitranscriptomic Microarray (8 × 60 K, Arraystar).

    Techniques: Microarray, Control, Methylation, Expressing