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dy417 deposited data microarray dataset peritoneal macrophages  (R&D Systems)


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    R&D Systems dy417 deposited data microarray dataset peritoneal macrophages
    Dy417 Deposited Data Microarray Dataset Peritoneal Macrophages, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 372 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+microarray+datasets/Mouse+IL-10+DuoSet+ELISA/pm32049012-206-216-213
    Average 96 stars, based on 372 article reviews
    dy417 deposited data microarray dataset peritoneal macrophages - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection.
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Recombinant:

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection.
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems DY485B-05 ELISA kits(IL-10) R&D Systems DY417-05 Dulbecco’s Modified Eagle Medium (DMEM) Gibco 11965092 Endothelial Cell Medium (ECM) ScienCell 1001 Fetal bovine serum (FBS) Gibco 10099141 Exosome-depleted FBS Gibco A2720803 Primers Sangon Biotech N/A Experimental models C57BL/6 mice Gempharmatech Co., Ltd RRID:IMSR_JAX:000664 Human Umbilical Vein Endothelial Cells Cellverse Co., Ltd RRID:CVCL_2959 HaCaT Keratinocytes Cellverse Co., Ltd RRID:CVCL_0038 Adipose-Derived Stem Cells SUNNCELL N/A Software and algorithms Malvern ZS Xplorer Malvern Panalytical Ltd. https://www.malvernpanalytical.com ImageJ National Institutes of Health RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 e1 iScience 29, 114560, February 20, 2026 Consideration of Sex Influence: This study utilized only male mice to minimize variability in wound healing responses that can be influenced by estrous cycle in females. ..

    Sterility:

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection.
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. In parallel, CD11b + Ly6G + Ly6C + cells were differentiated from WT or Thbs1 −/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b + Ly6G + Ly6C + cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO 2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO 2 for 4 h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice.
    Article Snippet: Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 and Leptospira biflexa serovar Patoc strain Patoc 1 were grown in Hornsby-Alt-Nally (HAN) media [67]. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), IL-10 (R&D Systems #DY417) and IL-6 (R&D Systems #DY406) following the manufacturers’ protocol. ..

    Article Title: Myeloid-derived interleukin-10 induced by thrombospondin-1 mediates host defense and regulates inflammation during acute bacterial lung infection.
    Article Snippet: To assess the role of TSP-1 in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0, 5, or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then, cells were washed with sterile RPMI media and stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 h. In parallel, CD11b+Ly6G+Ly6C+ cells were differentiated from WT or Thbs1−/− mice and stimulated with bacterial LPS (100 ng/mL) for 4 h. For each set of experiments, the supernatant was collected, and IL-10 production was measured by ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). .. To assess the role of PPARγ in IL-10 production, bone marrow CD11b+Ly6G+Ly6C+ cells were incubated with recombinant TSP-1 (0 or 15 μg/mL) at 37°C with 5% CO2 for 24 h. Then cells were then washed with sterile RPMI media and treated with vehicle (DMSO), the PPARγ agonist GW1929 (100 μM), or the PPARγ antagonist GW9662 (10 μM) for 1 h. All groups were stimulated with bacterial LPS (100 ng/mL) at 37°C with 5% CO2 for 4 Month XXXX Volume 0 Issue 0 10.1128/iai.00614-2513 D ow nl oa de d fr om h ttp s: //j ou rn al s. as m .o rg /jo ur na l/i ai o n 26 F eb ru ar y 20 26 b y 24 00 :1 a0 0: 4b ab :4 25 3: 45 cd :7 c9 8: fa 0a :f 53 9. h. The supernatant was collected for IL-10 measurement via ELISA (DuoSet ELISA Mouse IL-10, R&D Systems, DY417). ..

    Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice
    Article Snippet: For transfection of leptospiral DNA, 500 ng of DNA isolated from L. interrogans Fiocruz L1-130 cultures was incubated with Xfect transfection reagent (Takara Bio #631317) for 10 min and BMDMs were treated with the mixture for 6 h. After respective stimulations, supernatants were collected for quantification of cytokines and cells were preserved in RNAlater Stabilization Solution (Invitrogen #AM7020) for RNA isolation using RNeasy Mini kit (Qiagen #74106) as per the manufacturers’ instruction. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), IL-10 (R&D Systems #DY417) and IL-6 (R&D Systems #DY406) following the manufacturers’ protocol. ..

    Article Title: Structure matters: commensal Phocaeicola vulgatus lipopolysaccharide induces attenuated microglial activation and preserves neuronal integrity
    Article Snippet: Cytokine levels (pg/mL) in BV2 and HMC3 supernatants were measured following E. coli or P. vulgatus LPS stimulation using the ultrasensitive ELLA-Simple Plex platform (Protein Simple, Bio-Techne, San José, CA, United States), with a commercially available microfluidic cartridge for mouse (ST01C-MP-004435) and for human (ST01A-PS-003229) IL-1β, IL-6, and TNF-α detection. .. IL-10 was quantified using DuoSet ELISA Kit (R&D systems, Abingdon, United Kingdom) for mouse (#DY417) and for human (#DY217B) for BV2 and HMC3 supernatants, respectively. ..

    Article Title: Mincle-dependent Th17 adjuvanticity requires TNFR1 signaling in myeloid cells
    Article Snippet: .. For this purpose, the following ELISA kits were used according to the manufacturer’s instructions: IFNγ (R&D Systems, DY485), IL-17 (R&D Systems, DY421), IL-10 (R&D Systems, DY417), and IL-6 (R&D Systems, DY406). .. After blocking with anti-mouse CD16/32 antibody (eBioscience) the dead cells were stained with the Fixable Viability Dye eFluor 506 (Invitrogen 65-0866-14).

    Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems DY485B-05 ELISA kits(IL-10) R&D Systems DY417-05 Dulbecco’s Modified Eagle Medium (DMEM) Gibco 11965092 Endothelial Cell Medium (ECM) ScienCell 1001 Fetal bovine serum (FBS) Gibco 10099141 Exosome-depleted FBS Gibco A2720803 Primers Sangon Biotech N/A Experimental models C57BL/6 mice Gempharmatech Co., Ltd RRID:IMSR_JAX:000664 Human Umbilical Vein Endothelial Cells Cellverse Co., Ltd RRID:CVCL_2959 HaCaT Keratinocytes Cellverse Co., Ltd RRID:CVCL_0038 Adipose-Derived Stem Cells SUNNCELL N/A Software and algorithms Malvern ZS Xplorer Malvern Panalytical Ltd. https://www.malvernpanalytical.com ImageJ National Institutes of Health RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 e1 iScience 29, 114560, February 20, 2026 Consideration of Sex Influence: This study utilized only male mice to minimize variability in wound healing responses that can be influenced by estrous cycle in females. ..

    Cell Culture:

    Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice.
    Article Snippet: Leptospira interrogans serovar Copenhageni strain Fiocruz L1-130 and Leptospira biflexa serovar Patoc strain Patoc 1 were grown in Hornsby-Alt-Nally (HAN) media [67]. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), IL-10 (R&D Systems #DY417) and IL-6 (R&D Systems #DY406) following the manufacturers’ protocol. ..

    Article Title: cGAS-STING dependent type I IFN reduces Leptospira interrogans renal colonization in mice
    Article Snippet: For transfection of leptospiral DNA, 500 ng of DNA isolated from L. interrogans Fiocruz L1-130 cultures was incubated with Xfect transfection reagent (Takara Bio #631317) for 10 min and BMDMs were treated with the mixture for 6 h. After respective stimulations, supernatants were collected for quantification of cytokines and cells were preserved in RNAlater Stabilization Solution (Invitrogen #AM7020) for RNA isolation using RNeasy Mini kit (Qiagen #74106) as per the manufacturers’ instruction. .. Cytokines and chemokines were quantified in the cell culture supernatants using mouse ELISA kit for TNF (Invitrogen #88-7324-88), IFNβ (R&D Systems #DY8234), IL-10 (R&D Systems #DY417) and IL-6 (R&D Systems #DY406) following the manufacturers’ protocol. ..

    Electron Microscopy:

    Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems DY485B-05 ELISA kits(IL-10) R&D Systems DY417-05 Dulbecco’s Modified Eagle Medium (DMEM) Gibco 11965092 Endothelial Cell Medium (ECM) ScienCell 1001 Fetal bovine serum (FBS) Gibco 10099141 Exosome-depleted FBS Gibco A2720803 Primers Sangon Biotech N/A Experimental models C57BL/6 mice Gempharmatech Co., Ltd RRID:IMSR_JAX:000664 Human Umbilical Vein Endothelial Cells Cellverse Co., Ltd RRID:CVCL_2959 HaCaT Keratinocytes Cellverse Co., Ltd RRID:CVCL_0038 Adipose-Derived Stem Cells SUNNCELL N/A Software and algorithms Malvern ZS Xplorer Malvern Panalytical Ltd. https://www.malvernpanalytical.com ImageJ National Institutes of Health RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 e1 iScience 29, 114560, February 20, 2026 Consideration of Sex Influence: This study utilized only male mice to minimize variability in wound healing responses that can be influenced by estrous cycle in females. ..

    CCK-8 Assay:

    Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems DY485B-05 ELISA kits(IL-10) R&D Systems DY417-05 Dulbecco’s Modified Eagle Medium (DMEM) Gibco 11965092 Endothelial Cell Medium (ECM) ScienCell 1001 Fetal bovine serum (FBS) Gibco 10099141 Exosome-depleted FBS Gibco A2720803 Primers Sangon Biotech N/A Experimental models C57BL/6 mice Gempharmatech Co., Ltd RRID:IMSR_JAX:000664 Human Umbilical Vein Endothelial Cells Cellverse Co., Ltd RRID:CVCL_2959 HaCaT Keratinocytes Cellverse Co., Ltd RRID:CVCL_0038 Adipose-Derived Stem Cells SUNNCELL N/A Software and algorithms Malvern ZS Xplorer Malvern Panalytical Ltd. https://www.malvernpanalytical.com ImageJ National Institutes of Health RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 e1 iScience 29, 114560, February 20, 2026 Consideration of Sex Influence: This study utilized only male mice to minimize variability in wound healing responses that can be influenced by estrous cycle in females. ..

    Modification:

    Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems DY485B-05 ELISA kits(IL-10) R&D Systems DY417-05 Dulbecco’s Modified Eagle Medium (DMEM) Gibco 11965092 Endothelial Cell Medium (ECM) ScienCell 1001 Fetal bovine serum (FBS) Gibco 10099141 Exosome-depleted FBS Gibco A2720803 Primers Sangon Biotech N/A Experimental models C57BL/6 mice Gempharmatech Co., Ltd RRID:IMSR_JAX:000664 Human Umbilical Vein Endothelial Cells Cellverse Co., Ltd RRID:CVCL_2959 HaCaT Keratinocytes Cellverse Co., Ltd RRID:CVCL_0038 Adipose-Derived Stem Cells SUNNCELL N/A Software and algorithms Malvern ZS Xplorer Malvern Panalytical Ltd. https://www.malvernpanalytical.com ImageJ National Institutes of Health RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 e1 iScience 29, 114560, February 20, 2026 Consideration of Sex Influence: This study utilized only male mice to minimize variability in wound healing responses that can be influenced by estrous cycle in females. ..

    Software:

    Article Title: ADSC-derived exosomes deliver cerium oxide nanoparticles to promote diabetic wound healing via anti-inflammatory and pro-angiogenic effects.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD63 antibody Abcam Cat# ab134045; RRID: AB_2800495 Anti-CD81 antibody Abcam Cat# ab79559; RRID: AB_1603682 Anti-CD31 antibody Invitrogen Cat# MA5-37858; RRID: AB_2897778 Chemicals, peptides, and recombinant proteins Cerium(III) nitrate hexahydrate Sigma-Aldrich 238538-5G Ammonium hydroxide Sigma-Aldrich 221228-500 ML Ethanol Sigma-Aldrich 459836-1L Uranyl acetate (2%) Electron Microscopy Sciences 22400–2 Rhodamine B (Rho) Thermo Fisher Scientific R6626 DCFH-DA probe Sigma-Aldrich R6626 DAPI (4 ′ ,6-diamidino-2-phenylindole) Thermo Fisher Scientific D1306 Streptozotocin Sigma-Aldrich S0130-50 MG Paraformaldehyde (4%) Sigma-Aldrich 158127-500G CCK-8 assay kit Beyotime C0037 ELISA kits(IFN-γ) R&D Systems DY485B-05 ELISA kits(IL-10) R&D Systems DY417-05 Dulbecco’s Modified Eagle Medium (DMEM) Gibco 11965092 Endothelial Cell Medium (ECM) ScienCell 1001 Fetal bovine serum (FBS) Gibco 10099141 Exosome-depleted FBS Gibco A2720803 Primers Sangon Biotech N/A Experimental models C57BL/6 mice Gempharmatech Co., Ltd RRID:IMSR_JAX:000664 Human Umbilical Vein Endothelial Cells Cellverse Co., Ltd RRID:CVCL_2959 HaCaT Keratinocytes Cellverse Co., Ltd RRID:CVCL_0038 Adipose-Derived Stem Cells SUNNCELL N/A Software and algorithms Malvern ZS Xplorer Malvern Panalytical Ltd. https://www.malvernpanalytical.com ImageJ National Institutes of Health RRID:SCR_003070 GraphPad Prism GraphPad Software RRID:SCR_002798 e1 iScience 29, 114560, February 20, 2026 Consideration of Sex Influence: This study utilized only male mice to minimize variability in wound healing responses that can be influenced by estrous cycle in females. ..



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    Differential expression analysis of microarray data from experimental and human colorectal cancer tissue. ( A , B ) Principal component analysis (PCA) plots of public microarray datasets from mouse GSE86299 ( A ) and human GSE8671 ( B ) tissue, comparing normal colon (blue) and colorectal cancer (CRC) (red). Mouse n = 8 per group; human n = 32 per group. These are representative datasets of a total of three analyzed . ( C , D ) Volcano plots displaying differential gene expression comparing murine ( C ) and human ( D ) CRC tissue with adjacent normal tissue. Genes down- and upregulated in cancer tissue are highlighted in blue and red, respectively. ( E ) Venn diagram illustrating the most common up- and downregulated genes across three experimental mouse models of colitis-associated colorectal cancer (CAC) derived from Gene Expression Omnibus (GEO) datasets. ( F ) Venn diagram illustrating the most common up- and downregulated genes across three human CRC datasets from GEO. The analysis was performed using the limma package version 3.16.2 with cut-offs of false discovery rate ( FDR ) < 0.05 (Benjamini–Hochberg method) and log 2 F C (fold change) < − 1 and > 1 .

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    Article Title: Downregulation of Enteroendocrine Genes Predicts Survival in Colon Cancer: A Bioinformatics-Based Analysis

    doi: 10.3390/ijms262211127

    Figure Lengend Snippet: Differential expression analysis of microarray data from experimental and human colorectal cancer tissue. ( A , B ) Principal component analysis (PCA) plots of public microarray datasets from mouse GSE86299 ( A ) and human GSE8671 ( B ) tissue, comparing normal colon (blue) and colorectal cancer (CRC) (red). Mouse n = 8 per group; human n = 32 per group. These are representative datasets of a total of three analyzed . ( C , D ) Volcano plots displaying differential gene expression comparing murine ( C ) and human ( D ) CRC tissue with adjacent normal tissue. Genes down- and upregulated in cancer tissue are highlighted in blue and red, respectively. ( E ) Venn diagram illustrating the most common up- and downregulated genes across three experimental mouse models of colitis-associated colorectal cancer (CAC) derived from Gene Expression Omnibus (GEO) datasets. ( F ) Venn diagram illustrating the most common up- and downregulated genes across three human CRC datasets from GEO. The analysis was performed using the limma package version 3.16.2 with cut-offs of false discovery rate ( FDR ) < 0.05 (Benjamini–Hochberg method) and log 2 F C (fold change) < − 1 and > 1 .

    Article Snippet: We explored the presence or absence of secretory IEC markers by analyzing three different mouse CRC microarray datasets from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) database ( GSE86299 , GSE31105 , and GSE64658 ).

    Techniques: Quantitative Proteomics, Microarray, Gene Expression, Derivative Assay

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    Journal: Cell reports

    Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

    doi: 10.1016/j.celrep.2024.114149

    Figure Lengend Snippet: (A) Fold change in the expression of Thbs1 mRNA in soleus following 30 days of microgravity exposure (space flight) compared to control. Microarray dataset GEO: GSE80223 was analyzed from the Gene Expression Omnibus repository. Error bars denote ±SEM from n = 3 biologically independent animals per group; ** p < 0.01 by two-tailed unpaired Student’s t test. (B) RT-qPCR for Thbs1 , Thbs2 , Thbs3 , Thbs4 , and Thbs5 mRNA in the right tibialis anterior (TA) of 12-week-old wild-type (WT) mice subjected to 3 or 6 days (d) of unilateral hindlimb denervation, compared contralateral sham-operated leg. Error denote ±SEM from n = 3–5 biologically independent animals per group. * p < 0.05 by two-tailed unpaired Student’s t test. (C) Western blot for Thbs1 and Gapdh control in right TA of 12-week-old WT mice subjected to 3 or 10 days (d) of unilateral hindlimb denervation, compared to contralateral, sham-operated TA. n = 2 biologically independent animals per time point. (D) Representative immunohistochemistry for endogenous Thbs1 (green) and BiP (red) to visualize the endoplasmic reticulum (ER) on cryo-embedded TA of WT mice subjected to 10 days (d) of denervation compared to sham-operated controls at 12 weeks of age. Nuclei are shown in blue with DAPI. Scale bars represent 50 μm. (E) RT-qPCR for Thbs1 mRNA from TA of 8-week-old mice fed ad libitum or fasted for 48 h. Data are presented as fold expression over fed WT; error bars denote ±SEM from n = 4 biologically independent animals analyzed per group. * p < 0.05 by two-tailed unpaired Student’s t test. (F and G) Western blot for Thbs1 and Gapdh in TA of 8-week-old mice fed ad libitum or fasted for 48 h (F), and in young (12 weeks of age) and old (24 months of age) WT quadriceps (G). (H) Representative immunohistochemistry for endogenous Thbs1 (green), BiP (red), and the nucleus (DAPI, blue) on cryo-embedded 12-week-old (“young”) and 24-month-old (“old”) WT quadriceps. Scale bars represent 50 μm.

    Article Snippet: A literature search yielded a publicly available Affymetrix Mouse Genome 430A 2.0 microarray dataset (NCBI GEO Repository, GSE80223) in which Gambara G. et al. compared the gene expression adaptation in soleus from adult C57Bl/n6 mice that were flown in space aboard the BION-M1 biosatelite for 30 days in orbit ( n = 3), and from sex- and age-matched control mice that were housed in standard vivarium cages ( n = 3).

    Techniques: Expressing, Control, Microarray, Two Tailed Test, Quantitative RT-PCR, Western Blot, Immunohistochemistry

    Journal: Cell reports

    Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

    doi: 10.1016/j.celrep.2024.114149

    Figure Lengend Snippet:

    Article Snippet: A literature search yielded a publicly available Affymetrix Mouse Genome 430A 2.0 microarray dataset (NCBI GEO Repository, GSE80223) in which Gambara G. et al. compared the gene expression adaptation in soleus from adult C57Bl/n6 mice that were flown in space aboard the BION-M1 biosatelite for 30 days in orbit ( n = 3), and from sex- and age-matched control mice that were housed in standard vivarium cages ( n = 3).

    Techniques: Virus, Recombinant, Electron Microscopy, Plasmid Preparation, SYBR Green Assay, Membrane, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Protein Extraction, Clone Assay, Expressing, Software, Microscopy, Real-time Polymerase Chain Reaction