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h 1200 wash buffer  (Vector Laboratories)


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    Vector Laboratories h 1200 wash buffer
    H 1200 Wash Buffer, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mounting+buffer+dapi/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/pm41894395-234-35-32
    Average 98 stars, based on 22145 article reviews
    h 1200 wash buffer - by Bioz Stars, 2026-11
    98/100 stars

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    Incubation:

    Article Title: Integrated Reproductive and Cytogenetic Assessment of Infertility in a Captive Jaguar ( Panthera onca )
    Article Snippet: .. Specifically, the slides were aged at 65°C for 1 h, immersed in 0.4% HCl for 20 min, treated with 5% Ba(OH) 2 solution for 3 min at 45°C and incubated in 2× SSC for 1 h at 65°C, then rinsed in distilled water and counterstained with Vectashield DAPI anti‐fade medium (Vector Laboratories, Burlingame, CA, USA). .. G‐banding was performed sensu Seabright ( ).

    Article Title: Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
    Article Snippet: MDA-MB321 cells grown on glass slides in 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 (Sigma Aldrich, Steinheim, Germany) for 4 min. .. The slides were then incubated with rabbit polyclonal anti-hLFG antibody (1:100 dilution) and mouse polyclonal anti-h-ItdIns3,4,5P3 antibody (Invitrogen, Carlsbad, CA, USA) at 37◦C for 1 h. Slides were then washed three times with ice-cold PBS and incubated with Alexa Fluor 488- and Alexa Fluor 680-conjugated goat anti-rabbit and anti-mouse secondary antibodies (1:600 dilution each; Invitrogen, USA) at 37◦C for 30 min. After three additional washes with PBS, the samples were dried and mounted with Vectashield mounting medium (Vector Laboratories, Burlingame, CA) containing the DNA staining dye DAPI (4′,6′-diamidino-2-phenylindole). .. Images were captured using a ZEISS Axiovert 200 M fluorescence microscope equipped with the appropriate barrier filters.

    Automated:

    Article Title: Parvalbumin cell state dynamics regulate gamma oscillations and are disrupted in Alzheimer's disease.
    Article Snippet: Sections were then developed using diaminobenzidine (DAB; Vector Laboratories) for 6 minutes to enable chromogenic detection. .. Sections were mounted on slides, cover slipped with Vectashield antifade mounting medium with DAPI (Vector Laboratories, H-1200) or Cytoseal, and imaged with a Keyence BZ-X710 automated microscope. .. Quantification of amyloid deposits: Quantitative analysis of immunolabeled sections was conducted using ImageJ (NIH).

    Microscopy:

    Article Title: Parvalbumin cell state dynamics regulate gamma oscillations and are disrupted in Alzheimer's disease.
    Article Snippet: Sections were then developed using diaminobenzidine (DAB; Vector Laboratories) for 6 minutes to enable chromogenic detection. .. Sections were mounted on slides, cover slipped with Vectashield antifade mounting medium with DAPI (Vector Laboratories, H-1200) or Cytoseal, and imaged with a Keyence BZ-X710 automated microscope. .. Quantification of amyloid deposits: Quantitative analysis of immunolabeled sections was conducted using ImageJ (NIH).

    Staining:

    Article Title: Interaction of lifeguard (LFG/FAIM2) with phosphoinositides is associated with altered intracellular calcium homeostasis in breast cancer cells.
    Article Snippet: MDA-MB321 cells grown on glass slides in 4% paraformaldehyde for 20 min and permeabilized with 0.1% Triton X-100 (Sigma Aldrich, Steinheim, Germany) for 4 min. .. The slides were then incubated with rabbit polyclonal anti-hLFG antibody (1:100 dilution) and mouse polyclonal anti-h-ItdIns3,4,5P3 antibody (Invitrogen, Carlsbad, CA, USA) at 37◦C for 1 h. Slides were then washed three times with ice-cold PBS and incubated with Alexa Fluor 488- and Alexa Fluor 680-conjugated goat anti-rabbit and anti-mouse secondary antibodies (1:600 dilution each; Invitrogen, USA) at 37◦C for 30 min. After three additional washes with PBS, the samples were dried and mounted with Vectashield mounting medium (Vector Laboratories, Burlingame, CA) containing the DNA staining dye DAPI (4′,6′-diamidino-2-phenylindole). .. Images were captured using a ZEISS Axiovert 200 M fluorescence microscope equipped with the appropriate barrier filters.

    Glass:

    Article Title: Peroxisome-dependent and -independent functions of PEX19 in mouse embryonic stem cells
    Article Snippet: Cells were further blocked with 1% BSA and 0.05% Tween 20 in PBS for 30 minutes, incubated with primary antibodies diluted with the blocking solution for overnight at 4°C, then the secondary antibodies Alexa Fluor 488, 594 and 633 (for flow cytometry sorting) (Invitrogen, A-11008, A-11032, A-21052) at 1:1000 in PBS for 1 hour at room temperature. .. Cells were counterstained for Nuclei by directly mounting coverslips on glass slides with VECTASHIELD Antifade Mounting Media (VECTOR LABORATORY) which contains DAPI. .. The images of stained cells are acquired by Zeiss LSM 780 UV confocal microscope.



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