modified primer3 algorithm (Imgen Inc)

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Imgen Inc modified primer3 algorithm
Modified Primer3 Algorithm, supplied by Imgen Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/modified+primer3+algorithm/primer3+software/pmc02171373-182-7-10
Average 90 stars, based on 1 article reviews

modified primer3 algorithm - by Bioz Stars, 2026-10

90/100 stars

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Software:

Article Title: (Pro)Renin Receptor Decoy Peptide PRO20 Protects against Oxidative Renal Damage Induced by Advanced Oxidation Protein Products
Article Snippet: Transforming RNA into cDNA was performed using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). .. The primers were designed using Primer3 software ( http://flypush.imgen.bcm.tmc.edu/primer/primer3_www.cgi accessed on: 20 February 2023) ( ). .. Light Cycler 480 Real-Time PCR System was used to perform qPCR using Roche’s SYBR Green Master Mix (Roche). qPCR reaction systems and conditions are shown in .

Article Title: (Pro)renin receptor mediates albumin-induced cellular responses: role of site-1 protease-derived soluble (pro)renin receptor in renal epithelial cells
Article Snippet: .. Oligonucleotides were designed using Primer3 software (available at http://flypush.imgen.bcm.tmc.edu/primer/primer3_www.cgi ) and are shown in . table ft1 table-wrap mode="anchored" t5 Table 2. caption a7 Target Gene Primer Sequence Human-GAPDH, F TCATTGACCTCAACTACATG Human-GAPDH, R CAAAGTTGTCATGGATGACC Human-PRR, F CAGACGTGGCTGCATTGTCC Human-PRR, R CTGGGGGTAGAGCCAGTTTGTT Human-ACE, F GGAGGAATATGACCGGACATCC Human-ACE, R TGGTTGGCTATTTGCATGTTCTT Human-Furin, F GCAATAATGGTCCCCATCC Human-Furin, R GCCTCCTCCTTCCAATATCC Human-ADAM19, F AAGTACCATGACAACGCCCAAT Human-ADAM19, R CATTCTCGGAGTGGTCCATGT Human-PCSK1, F CTGGATGGCATTGTGACGGAT Human-PCSK1, R GCCCCAGCTTGCACTGTAAA Human-PCSK2, F GGGAAAGGTGTTACCATTGGAA Human-PCSK2, R CCAGTCATCTGTGTACCGAGG Human-PCSK4, F CACGCAAATTCGGCTTCGTC Human-PCSK4, R ACGACAGAGCGTTTCACCC Human-PCSK5, F CCAACCACTGGGCAGTCAAA Human-PCSK5, R CTGACCTTTTAATCGTCCTGCT Human-PCSK6, F CGCAGGCCCTTTACTTCAAC Human-PCSK6, R CGGCAGCGACTGTTCTTGT Human-PCSK7, F GCAGCGTCCACTTCAACGA Human-PCSK7, R GCCCAGTCACATTGCGTTC Human-PCSK9, F AGACCCACCTCTCGCAGTC Human-PCSK9, R GGAGTCCTCCTCGATGTAGTC Human-S1P, F ACCTCGAAACAATCCATCCAGT Human-S1P, R ACTTGAGGGAACGAAAGACTTTT Human-TGF-β1, F TCCACCTGCAAGACTATCGAC Human-TGF-β1, R GAGGTATCGCCAGGAATTGTT Human-IL-6, F ACTCACCTCTTCAGAACGAATTG Human-IL-6, R CCATCTTTGGAAGGTTCAGGTTG Human-IL-8, F TTTTGCCAAGGAGTGCTAAAGA Human-IL-8, R AACCCTCTGCACCCAGTTTTC Open in a separate window F, forward; R, reverse; PRR (ATP6AP2), prorenin receptor; ACE, angiotensin-converting enzyme; ADAM19, a disintegrin and metalloproteinase domain 19; PCSK1/2/4/5/6/7/9, Homo sapiens proprotein convertase subtilisin/kexin type 1/2/4/5/6/7/9; S1P, also known as SKI-1, membrane-bound transcription factor peptidase, site-1; TGF-β1: transforming growth factor-β1. ..

Article Title: Enhanced Secondary- and Hormone Metabolism in Leaves of Arbuscular Mycorrhizal Medicago truncatula
Article Snippet: .. Sequences of primers designed using Primer 3 software ( flypush.imgen.bcm.tmc.edu/primer/primer3_www.cgi ) are given in Supplemental Table S12 . .. Total RNA was used as a template to synthesize cDNA using the iScript cDNA kit according to the manufacturer’s instructions (Bio-Rad Laboratories).

Article Title: (Pro)Renin Receptor Decoy Peptide PRO20 Protects against Oxidative Renal Damage Induced by Advanced Oxidation Protein Products.
Article Snippet: Transforming RNA into cDNA was performed using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). .. The primers were designed using Primer3 software (http://flypush.imgen.bcm.tmc.edu/primer/primer3_www.cgi accessed on: 20 February 2023) (Table 1). .. Light Cycler 480 Real-Time PCR System was used to perform qPCR using Roche’s SYBR Green Master Mix (Roche). qPCR reaction systems and conditions are shown in Table 2.

Sequencing:

Membrane:

Real-time Polymerase Chain Reaction:

Article Title: A Novel 1.0 Mb Duplication of Chromosome 8p22-21.3 in a Patient With Autism Spectrum Disorder
Article Snippet: .. The primer pairs of each gene for quantitative PCR were designed using the Primer3 ( http://flypush.imgen.bcm.tmc.edu/primer/primer3_www.cgi ; Supplemental Table S1). ..

Article Title: Identification of optimal reference genes for quantitative PCR studies on human mesenchymal stem cells.
Article Snippet: .. Primer pairs used for qPCR were designed using primer three input (http://flypush.imgen.bcm.tmc.edu/primer/primer3_www. cgi). .. The qPCR primers were synthesized by Sigma Genesys (Sigma-Aldrich) with melting temperature (Tm) at 60±1 ̊C.

Synthesized:

Article Title: Umbilical Cord Tissue-Derived Mesenchymal Stem Cells Induce T Lymphocyte Apoptosis and Cell Cycle Arrest by Expression of Indoleamine 2, 3-Dioxygenase
Article Snippet: .. Primers were designed using Primer3 Input ( http://flypush.imgen.bcm.tmc.edu/primer/primer3_www.cgi ) and synthesized with a T m at 60°C. ..

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