miscript ii rt kit (Qiagen)

Qiagen is a verified supplier

Qiagen manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Qiagen miscript ii rt kit
Miscript Ii Rt Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 6771 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+kit/RNase+A/pmc07716711-83-5-9
Average 99 stars, based on 6771 article reviews

miscript ii rt kit - by Bioz Stars, 2026-09

99/100 stars

Images

Lysis:

Article Title: A new genome assembly of the pea cultivar 'Caméor' provides resources for functional genomics and genetics.
Article Snippet: .. After overnight proteinase K digestion in the presence of Lysis Buffer and one hour treatment with RNAse A (Qiagen), plugs were washed three times in 1x Wash Buffer and three times in 1x TE Buffer (ThermoFisher Scientific). .. Then, plugs were melted two minutes at 70°C and solubilized with 2 μL of 0.5 U/μL AGARase enzyme (ThermoFisher Scientific) for 45 minutes at 43°C.

FACS:

Article Title: Proteomic profiling of ANO10-deficient SH-SY5Y cells reveals candidate pathways relevant to SCAR10.
Article Snippet: .. AR TIC LE IN PR ES S ARTICLE IN PRESS two washes with FACS buffer [0.5% bovine serum albumin in PBS], cells were pelleted (300 × g, 5 minutes, 4°C), resuspended in 500 μL of PI-staining solution [20 μg/mL propidium iodide (Thermo Fisher Scientific), 200 μg/mL RNase A (Qiagen N.V.), 2 mM MgCl2 in FACS buffer], and incubated for 20 minutes at room temperature in the dark. ..

Incubation:

Article Title: Mre11-Rad50 enhances spacer acquisition in a haloarchaeal Type I-B CRISPR-Cas system
Article Snippet: To a fresh or freshly thawed pellet, we add 10 mL of lysis solution (100 mM EDTA pH 8.0, 0.2% SDS) and 200 μL Proteinase K (ThermoFisher, Cat #EO0492) and vortexed vigorously. .. Lysates were incubated at 56°C for 10 min, vortexing occasionally, then treated with 40 μL RNase A (Qiagen, Cat #19101) and incubated at room temperature for 10 min. An equal volume of phenol:choloform:isoamyl alcohol (Millipore Sigma, Cat #P2069) was added to the lysate and vortexed for 30 sec. After centrifugation at 12000 rpm for 10 min at room temperature, the top aqueous layer was transfered to a fresh 50 mL Falcon tube. .. To each aqueous solution, we add 80 μL GlycoBlue coprecipitant (ThermoFisher, Cat #AM9515), 2.5 mL NH 4 OAc (7.5 M), and 18.75 mL cold 100% ethanol, and vortex.

Article Title: Quantitative PCR-Based Analysis of Bacterial Profiles in Periapical Lesions and Maxillary Sinus in Odontogenic Sinusitis.
Article Snippet: .. Freshly prepared lysozyme (100 μL, stock 100 mg/mL, Thermo Fisher Scientific), RNase A (10 μL, from DNA Extraction Genomic Mini, Blirt) and freshly prepared lysostaphin (8 μL, stock 10 mg/mL, Sigma-Aldrich) were added and the samples were incubated for 40 min at 37 ◦C. .. DNA was extracted using a DNA Extraction Genomic Mini kit (Blirt, Gdańsk, Poland).

Suspension:

Article Title: Nuclear genome profiling of two species of Epidendrum (Orchidaceae): genome size, repeatome, and ploidy
Article Snippet: .. The suspension was filtered through a 30–50 μm pore CellTricsTM filter (SYSMEX; https://www.sysmex.com ) and treated with 50 μg/ml RNAse A (Qiagen) and 50 μg/ml propidium iodide (Sigma–Aldrich). ..

Centrifugation:

Article Title: Comparative genomic analysis of Acinetobacter baumannii isolates from seafood markets and healthcare environments: a case study from Kerala, India
Article Snippet: Genomic DNA (gDNA) was extracted using the QIAGEN Genomic-tip 20/G kit following the manufacturer’s protocol with minor modifications. .. Briefly, 1.5 mL samples of saturated bacterial cultures were pelleted, lysed using proteinase K and RNase A, cleared by centrifugation, and applied to Genomic-tip columns pre-equilibrated with QBT buffer (QIAGEN). .. Columns were washed with QC buffer (QIAGEN), and gDNA was eluted using QF buffer (QIAGEN), precipitated with isopropanol, washed with 70% ethanol, and resuspended in Tris-EDTA buffer.

Formalin-fixed Paraffin-Embedded:

Article Title: Machine Learning Analysis to Define Cell Lineage in Leiomyosarcoma
Article Snippet: .. DNA was extracted from 2mm tissue cores using AllPrep DNA/RNA FFPE Kit, including RNase A treatment, according to the manufacturer’s protocol (Qiagen). .. DNA methylation profiling was performed using the Illumina Infinium MethylationEPIC BeadChip array as previously described .

Staining:

Article Title: P27 single-nucleotide polymorphism T2871099G as a predictor of the benefit of endocrine therapy alone or in combination with CDK inhibitors in breast cancer
Article Snippet: Fixed cells were treated with 2 M HCl for 20 min, and BrdU was immunolabeled with FITC-conjugated anti-BrdU (Cat. 556028, BD PharmigenTM). .. For DNA-content analysis, cells were fixed in 70% ethanol, washed in PBS and stained with 50 μg/ml propidium iodide (Sigma) in the presence of 10 μg/ml RNase A (Qiagen). .. Flow cytometry data were acquired in a FACSCanto cytometer (BD Biosciences) and analyzed with FlowJo software (Tree Star Inc.).

Review