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reverse transcription kit  (Qiagen)


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    Structured Review

    Qiagen reverse transcription kit
    Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1089 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/miscript+rt+kit/QuantiNova+Reverse+Transcription+Kit/pm36114541-51-10-17
    Average 96 stars, based on 1089 article reviews
    reverse transcription kit - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival
    Article Snippet: .. Total RNA from MSCs was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, catalog# 74134,), and the reverse transcription was performed with QuantiNova Reverse Transcription Kit (Qiagen, catalog# 205411) according to the manufacturer’s instructions. .. Quantitative PCR was conducted to assess target mRNA levels using TaqMan Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA, catalog# 4444963) and Gene Expression TaqMan Assays (Applied Biosystems) targeting VEGFA (Exon 3–4) (Hs00900055_m1) and GAPDH (Hs02758991_g1) as per the manufacturer’s protocols.

    Article Title: Trans -Chalcone Reduces Inflammation and Pain Triggered by Superoxide Anion: Neuronal and Non-Neuronal Mechanisms
    Article Snippet: .. The QuantiTect Reverse Transcription kit (QIAGEN, #205314) was used to obtain cDNA, and the QuantiNova SYBR Green (QIAGEN, #208056) kit was used for quantitative PCR analysis. ..

    Article Title: Extracellular Vesicles Derived from VEGF mRNA-Engineered Mesenchymal Stem Cells Promote Endothelial Cell Survival.
    Article Snippet: .. Total RNA from MSCs was extracted using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany, catalog# 74134,), and the reverse transcription was performed with QuantiNova Reverse Transcription Kit (Qiagen, catalog# 205411) according to the manufacturer’s instructions. .. Quantitative PCR was conducted to assess target mRNA levels using TaqMan Fast Advanced Master Mix (Applied Biosystems, Waltham, MA, USA, catalog# 4444963) and Gene Expression TaqMan Assays (Applied Biosystems) targeting VEGFA (Exon 3–4) (Hs00900055_m1) and GAPDH (Hs02758991_g1) as per the manufacturer’s protocols.

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    Article Title: Epigenetic and microRNA-mediated regulation of pulmonary fibrosis and immune dysregulation in fatal COVID-19.
    Article Snippet: Total RNA was extracted from the lung samples using TRIzol (Invitrogen) following tissue disruption with the TissueLyser LT (Qiagen). .. Approximately 2 μg of RNA was treated with Turbo DNAse I (Invitrogen), followed by cDNA synthesis using the QuantiNova Reverse Transcription Kit (Qiagen). .. The cDNAs were subjected to RT‒qPCR using 1x SYBR Green Universal Master Mix (Applied Biosystems) on a StepOnePlus system (Applied Biosystems).

    Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling
    Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the QuantiNova Reverse Transcription Kit (205411, Qiagen). .. Quantitative real-time PCR (RT-qPCR) was performed on a Rotor-Gene Q system (Qiagen) followed by analyses using Rotor-Gene Q software (version 2.3.5).

    Article Title: Co-culturing matrigel-encapsulated post-cryopreserved human ovarian cortical tissue with mesenchymal stem cells to improve In situ follicular activation and early development.
    Article Snippet: Recent advances in culturing cryopreserved ovarian cortical tissue (OCT) offer a promising strategy for fertility preservation, especially for patients undergoing gonadotoxic treatments.. This study evaluated whether Matrigel encapsulation combined with mesenchymal stem cell (MSC) co-culture enhances folliculogenesis in postcryopreserved human OCT during an 8-day in vitro culture.. Thawed human OCT fragments were encapsulated in Matrigel and cultured in the upper chamber of a transwell plate, with MSCs in the lower chamber.

    SYBR Green Assay:

    Article Title: Trans -Chalcone Reduces Inflammation and Pain Triggered by Superoxide Anion: Neuronal and Non-Neuronal Mechanisms
    Article Snippet: .. The QuantiTect Reverse Transcription kit (QIAGEN, #205314) was used to obtain cDNA, and the QuantiNova SYBR Green (QIAGEN, #208056) kit was used for quantitative PCR analysis. ..

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Trans -Chalcone Reduces Inflammation and Pain Triggered by Superoxide Anion: Neuronal and Non-Neuronal Mechanisms
    Article Snippet: .. The QuantiTect Reverse Transcription kit (QIAGEN, #205314) was used to obtain cDNA, and the QuantiNova SYBR Green (QIAGEN, #208056) kit was used for quantitative PCR analysis. ..

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    Isolation:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Synthesized:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling
    Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the QuantiNova Reverse Transcription Kit (205411, Qiagen). .. Quantitative real-time PCR (RT-qPCR) was performed on a Rotor-Gene Q system (Qiagen) followed by analyses using Rotor-Gene Q software (version 2.3.5).

    Quantitative RT-PCR:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Expressing:

    Article Title: CaMKIV negatively regulates osteoblast differentiation by modulating c-Fos and NFATc1 signaling: an in vitro and in vivo mechanistic study
    Article Snippet: .. Total RNA was isolated using TRIzol (Invitrogen, Waltham, MA, USA), and cDNA was synthesized with the QuantiNova Reverse Transcription Kit (Qiagen GmbH, Hilden, Germany). qRT-PCR was performed using SYBR Green master mix (Qiagen), and relative expression was calculated by the ∆∆Ct method. ..

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    Purification:

    Article Title: Dietary heat-killed Limosilactobacillus ingluviei C37 postbiotic improves growth performance and modulates stress-related gene expression in heat-stressed broiler chickens
    Article Snippet: The concentration and purity of the extracted RNA were assessed using a NanoVueTM Plus spectrophotometer (GE Healthcare, Singapore) by measuring the absorbance ratio at 260/280 nm. .. The purified RNA was then converted into complementary DNA (cDNA) using the QuantiNova® reverse transcription kit (Qiagen, Hilden, Germany) for quantitative real-time polymerase chain reaction (qPCR) analysis, as described by . qPCR was performed in triplicate on a LightCycler® 480 II system (Roche, Mannheim, Germany) to quantify the expression levels of target genes. ..

    cDNA Synthesis:

    Article Title: Epigenetic and microRNA-mediated regulation of pulmonary fibrosis and immune dysregulation in fatal COVID-19.
    Article Snippet: Total RNA was extracted from the lung samples using TRIzol (Invitrogen) following tissue disruption with the TissueLyser LT (Qiagen). .. Approximately 2 μg of RNA was treated with Turbo DNAse I (Invitrogen), followed by cDNA synthesis using the QuantiNova Reverse Transcription Kit (Qiagen). .. The cDNAs were subjected to RT‒qPCR using 1x SYBR Green Universal Master Mix (Applied Biosystems) on a StepOnePlus system (Applied Biosystems).

    Cell Culture:

    Article Title: Molecular role of developmentally regulated GTP-binding protein 1 in coordinating osteoclast and osteoblast differentiation during bone remodeling
    Article Snippet: Signals were detected, visualized using ECL detection reagents (Millipore Corporation), and analyzed with an Azure 300 luminescent imaging system (Azure Biosystems, Dublin, CA, USA). .. Total RNA was extracted from cultured cells using Qiazol reagent (Qiagen GmbH, Hilden, Germany), and complementary DNA (cDNA) was synthesized using the QuantiNova Reverse Transcription Kit (205411, Qiagen). .. Quantitative real-time PCR (RT-qPCR) was performed on a Rotor-Gene Q system (Qiagen) followed by analyses using Rotor-Gene Q software (version 2.3.5).



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